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Identification of major carotenoids from green alga Tetraspora sp. CU2551: partial purification and characterization of lutein, canthaxanthin, neochrome, and β-carotene
(2022-08-01)
Maswanna, Thanaporn
;
Maneeruttanarungroj, Cherdsak
The green algae Tetraspora sp. CU2551 was previously identified as a strain with high potential for biohydrogen production; however, its algal biomass characteristics changed from green to reddish orange within 43 days of biohydrogen production. The crude pigments were extracted, partially purified, and characterized by chemical determination. The present study focused on elucidating the carotenoid composition of the selected green alga Tetraspora sp. CU2551. The pigment extract was partially purified and fractionated using thin layer chromatography, and yielded two major and two minor carotenoid bands. The fractions were confirmed by high-performance liquid chromatography with a diode array detector (HPLC–DAD) before being identified and confirmed using Liquid Chromatograph-Quadrupole Time of Flight-Mass Spectrometry (LC-QTOF-MS). The spectral data of these fractions revealed four sub-fractions of interest that were lutein, canthaxanthin, neochrome, and β-carotene, which had percentages in the crude extracts of 30.57%, 25.47%, 7.89%, and 0.71%, respectively. Lutein and canthaxanthin were found to be the major carotenoid pigments present. Our findings in this present study are the first reporting of Tetraspora sp. CU2551 as a potential alternate source for carotenoid pigment production.
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Improved biohydrogen production by immobilized cells of the green alga Tetraspora sp. CU2551 incubated under aerobic condition
(2020-10-01)
Maswanna, Thanaporn
;
Lindblad, Peter
;
Maneeruttanarungroj, Cherdsak
The green alga Tetraspora sp. CU2551 was previously investigated and showed enhanced H<inf>2</inf> production under anaerobic (Ar purged) conditions by cells immobilized in a calcium alginate matrix (Maswanna et al., Biomass Bioenergy 111:88–95, 2018). Here, we report successful H<inf>2</inf> production in entrapped cells under aerobic conditions. The most favorable immobilization condition observed was 4% (w/v) final alginate concentration after gelation, 2.80- to 3.35-mm beads, and a biomass content of 0.125 mg DW mL−1 alginate. H<inf>2</inf> production increased when the immobilized cells were incubated in S-deprived media which could be repeated up to six times when using refreshed media. After six cycles, the H<inf>2</inf> production reached 12.8 ± 0.9 mL H<inf>2</inf> 25 mL−1 of medium, corresponding to a rate of 182 ± 20 nmol mg−1 DW h−1, which was significantly higher than previously observed for other microalgae. Thus, our results demonstrate a potential for photobiological H<inf>2</inf> production using immobilized Tetraspora sp. CU2551 cells, grown under ambient aerobic conditions.
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Enhanced hydrogen production by optimization of immobilized cells of the green alga Tetraspora sp. CU2551 grown under anaerobic condition
(2018-04-01)
Maswanna, Thanaporn
;
Phunpruch, Saranya
;
Lindblad, Peter
;
Maneeruttanarungroj, Cherdsak
The green alga Tetraspora sp. CU2551 has previously been identified and characterized as a photosynthetic microorganism with high potential for H<inf>2</inf> production. In the present study, cells of Tetraspora CU2551 were entrapped and immobilized in an alginate matrix with the aim to analyze the effect of cell stacking and a reduced exposure of O<inf>2</inf> to the cells. The results showed that the most favorable immobilization conditions were 4% (w/v) of final alginate concentration and a cell concentration of 0.125 mg cell dry wt/mL alginate with a bead diameter of 2.80–3.35 mm. The H<inf>2</inf> production yields increased when the immobilized cells were incubated in S-deprived medium and this could be repeated at least for 3 production times. Maximal total H<inf>2</inf> production reached 7.68 ± 0.88 mL H<inf>2</inf>/25 mL medium, corresponding to a rate of 1182.45 ± 24.40 nmol H<inf>2</inf>/h/mg DW. This production is about 6 times higher compared to by cells in suspension, 2–10 times higher when compared to by other green algae, and 10–50 times higher when comparing with cyanobacteria. Based on our observations, immobilized cells of Tetraspora CU2551 is considered a very promising biological system for significant photobiological H<inf>2</inf> production by a photosynthetic microorganism.

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