Search Results

Now showing 1 - 2 of 2

Some of the metrics are blocked by your
consent settings
Simple and fast fabrication of microfluidic paper-based analytical device by contact stamping for multiple-point standard addition assay: Application to direct analysis of urinary creatinine
(2020-04-01)
Mathaweesansurn, Arjnarong
;
Thongrod, Suthathip
;
Khongkaew, Putthiporn
;
Phechkrajang, Chutima Matayatsuk
;
Wilairat, Prapin
In this work, a microfluidic paper-based analytical device (μPAD) for simultaneous multiple-point standard addition assay was fabricated by rubber stamping the hydrophobic barrier pattern onto a laboratory filter paper. The μPAD has a central zone from which an applied sample flows into eight surrounding narrow channels to which had been added the standard solutions. Each channel is connected to a circular area loaded with the reagent. The opposite end of this reagent zone is connected by second narrow channel to the final circular detection zone. The μPAD was applied to the measurement of creatinine in human urine. After addition of a urine sample, the orange-colored product arising from the Jaffé reaction is formed at the eight detection zones. A digital image of the μPAD is then recorded and the ratio of the red/green (R/G) intensity obtained using the ImageJ™ program is used in the quantitation of creatinine. The normal standard addition calibration line is constructed using the intensity ratio against the added creatinine concentrations (50–1000 mg L−1). Good linearity was achieved (r2 ˃ 0.99). There were no significant differences between the creatinine content using an HPLC method (paired t-test at 95% confidence, t<inf>stat</inf> = 1.78, t<inf>critical</inf> = 2.26, n = 10). The use of simultaneous multiple-point standard addition calibration allows rapid determination of creatinine in urine with elimination of matrix interference.
Some of the metrics are blocked by your
consent settings
Low-cost synthesis of gold nanoparticles from reused traditional gold leaf and its application for sensitive and selective colorimetric sensing of creatinine in urine
(2020-01-01)
Mathaweesansurn, Arjnarong
;
Choengchan, Nathawut
;
Khongkaew, Putthiporn
;
Phechkrajang, Chutima M.
Background: Gold nanoparticles (Au NPs) are normally prepared using standard gold (III) trichloride which is much expensive and irritant. This work is aimed at demonstrating simple and low-cost synthesis of Au NPs from the reused traditional gold leaf which is cost-free and less toxic. Methods: The reused gold leaf was donated by the local temple. It was digested and used as the precursor for the preparation of the Au NPs by Turkevich method. Poly (vinyl alcohol) (PVA) was em-ployed as a stabilizer. The as-prepared Au NPs were applied for the colorimetric determination of creatinine in urine without any sample pretreatment. Results: Long-term stability of the gold colloids was achieved for at least 3 months. Morphology and purity of the as-prepared Au NPs were the same as the ones prepared from standard gold (III) salt and standard gold foil. Colorimetric response of the Au NPs was linear to the standard creatinine up to 200 mg L-1. The limit of detection (0.16 mg L-1 or 1.41 µM) was enough sensitive for urinary cre-atinine detection in patients with kidney disease. Good recoveries (97-108%) and fast analysis time (3 min) were achieved. The developed method was successfully validated against the HPLC method. Conclusion: Facile and cost-effective synthesis of the Au NPs from the reused traditional gold leaf, was accomplished. The as-prepared Au NPs were successfully applied for the determination of urinary creatinine with high sensitivity and selectivity.

KMITL

Browse

Filters

Settings

Sort By

Results per page

Search Results