KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/kmitl/1

Browse

Settings

Author: search.filters.author.Nammali, AchararakSubject: search.filters.subject.16S rRNA gene

Search Results

Now showing 1 - 4 of 4
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Nocardia coffeae sp. nov., an endophytic actinobacterium isolatefrom the root of Coffea arabica (L.)
    (2022-01-01)
    Nammali, Achararak
    ;
    Duangupama, Thitikorn
    ;
    Suriyachadkun, Chanwit
    ;
    Tanasupawat, Somboon
    ;
    Thawai, Chitti
    The polyphasic taxonomic study of a novel endophytic actinobacterium strain (CA2R105<sup>T</sup>) was carried out. The strain formed fragmented substrate mycelium and showed chemotaxonomic properties typical of members of the genus Nocardia, i.e. the presence of mycolic acid and MK-8 (H<inf>4ω-cycl</inf> ) in its cells. Strain CA2R105<sup>T</sup> exhibited the highest 16S rRNA gene sequence similarity to Nocardia jiangxiensis NBRC 101359<sup>T</sup> (99.2%). The genome-based taxonomic analysis revealed low average nucleotide identity-blast and digital DNA–DNA hybridization values (<93.7, and <65.2%, respectively) to its closest relative. Moreover, many different phenotypic characteristics were observed between strain CA2R105<sup>T</sup> and all related Nocardia-type strains. This taxonomic evidence suggested that strain CA2R105<sup>T</sup> should be judged as representing a novel species of the genus Nocardia and the name, Nocardia coffeae sp. nov. is proposed. The type strain is CA2R105<sup>T</sup> (=TBRC 11247<sup>T</sup>=NBRC 114292<sup>T</sup>).
      2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Streptomyces acidipaludis sp. nov., an actinobacterium isolated from peat swamp forest soil
    (2022-01-01)
    Nammali, Achararak
    ;
    Duangupama, Thitikorn
    ;
    Pittayakhajonwut, Pattama
    ;
    Intaraudom, Chakapong
    ;
    Suriyachadkun, Chanwit
    An actinobacterium strain PLK6-54<sup>T</sup> was isolated from Lankwai peat swamp forest soil collected from Yala province, Thailand. Strain PLK6-54<sup>T</sup> exhibited morphological and chemotaxonomic properties described for the genus Streptomyces. It formed a spiral spore chain directly on aerial mycelium. Growth was observed between 20 and 40 °C and at pH 5–8. The maximum NaCl for growth was 2% (w/v). ll-Diaminopimelic acid, arabinose and ribose were detected in the whole-cell hydrolysate. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside were detected as the phospholipids. The major menaquinones were MK-10(H<inf>2</inf>) and MK-9(H<inf>6</inf>). The major cellular fatty acids were iso-C<inf>16:0</inf>, anteiso-C<inf>15:0</inf> and iso-C<inf>14:0</inf>. 16S rRNA gene sequence data supported the assignment of strain PLK6-54<sup>T</sup> to the genus Streptomyces and showed that Streptomyces rubidus NBRC 102073<sup>T</sup> (99.0%) was the closest relative. Moreover, the average nucleotide identity-blast (85.5%) and digital DNA–DNA hybridization (30.7%) values reported between strain PLK6-54<sup>T</sup> and its closest neighbour were below the threshold values for delineation of a novel species. Strain PLK6-54<sup>T</sup> could be distinguished from related validly described Streptomyces species by several phenotypic properties. The combination of genotypic and phenotypic data indicated that strain PLK6-54<sup>T</sup> is representative of a novel species of the genus Streptomyces. The name Streptomyces acidipaludis sp. nov. is proposed for strain PLK6-54<sup>T</sup>. The type strain is PLK6-54<sup>T</sup> (=TBRC 11250<sup>T</sup>=NBRC 114297<sup>T</sup>).
      1
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Streptomyces endocoffeicus sp. nov., an endophytic actinomycete isolated from Coffea arabica (L.)
    (2021-11-01)
    Nammali, Achararak
    ;
    Intaraudom, Chakapong
    ;
    Pittayakhajonwut, Pattama
    ;
    Suriyachadkun, Chanwit
    ;
    Tadtong, Sarin
    An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110<sup>T</sup>, was isolated from the surface-sterilised root of Coffea arabica L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110<sup>T</sup> was a member of the genus Streptomyces and showed the closest similarities to Streptomyces buecherae AC541<sup>T</sup> (99.2%), followed by Streptomyces rapamycinicus NRRL B-5491<sup>T</sup> (99.1%), Streptomyces luteoverticillatus NBRC 3840<sup>T</sup> (99.1%), Streptomyces coerulescens NBRC 12758<sup>T</sup> (99.1%), and Streptomyces iranensis HM 35<sup>T</sup> (99.0%). Strain CA3R110<sup>T</sup> contained LL-diaminopimelic acid in cell peptidoglycan, MK-9(H<inf>6</inf>), and MK-9(H<inf>8</inf>) as major menaquinone, iso-C<inf>16:0,</inf>iso-C<inf>15:0,</inf> C<inf>16:0</inf> as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside were detected in the cell. The chemotaxonomic characteristics possessed the typical properties of the genus Streptomyces. A low digital DNA–DNA hybridization (< 55.7%) and average nucleotide identity-blast (ANIb) (< 92.2%) values revealed that strain CA3R110<sup>T</sup> could be distinguished from any known Streptomyces species. With the differences in phenotypic and genotypic data, strain CA3R110<sup>T</sup> represents a novel species of genus Streptomyces, for which the name Streptomyces endocoffeicus sp. nov. is proposed. The type strain is CA3R110<sup>T</sup> (= TBRC 11245<sup>T</sup> = NBRC 114296<sup>T</sup>).
      2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Streptomyces coffeae sp. Nov., an endophytic actinomycete isolated from the root of coffea arabica (l.)
    (2021-01-01)
    Nammali, Achararak
    ;
    Intaraudom, Chakapong
    ;
    Pittayakhajonwut, Pattama
    ;
    Suriyachadkun, Chanwit
    ;
    Tadtong, Sarin
    An endophytic actinobacterium, designated strain CA1R205<sup>T</sup>, was isolated from the surface-sterilized root of Coffea arabica L. collected from Ratchaburi province, Thailand. The taxonomic position of this strain was evaluated using a polyphasic approach. The strain produced light yellowish brown to dark brownish black substrate mycelium and greyish white aerial mycelium. The spiral spore chains were produced directly on aerial mycelium. CA1R205<sup>T</sup> was found to have ll-diaminopimelic acid in the cell peptidoglycan, galactose, glucose, mannose and ribose as whole-cell reducing sugars, MK-10(H<inf>4</inf>), MK-9(H<inf>6</inf>), MK-10(H<inf>2</inf>), MK-9(H<inf>4</inf>), MK-10(H<inf>6</inf>) and MK-10(H<inf>8</inf>) as menaquinones and iso-C<inf>15:0</inf>, anteiso-C<inf>15:0</inf>, iso-C<inf>16:0</inf> and C<inf>16:0</inf> as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were detected in the cells. These characteristics were consistent the typical chemotaxonomic properties of members the genus Streptomyces. The taxonomic affiliation at the genus level of this strain could be confirmed using its 16S rRNA gene sequence data. CA1R205<sup>T</sup> showed the highest 16S rRNA gene sequence similarity value to Streptomyces rapamycinicus NRRL B-5491<sup>T</sup> (98.9%), followed by Streptomyces iranensis HM 35<sup>T</sup> (98.8%). Digital DNA–DNA hybridization and average nucleotide identity-by blast (ANIb) values between CA1R205<sup>T</sup> and S. rapamycinicus NRRL B-5491<sup>T</sup> were 27.2 and 81.5%, respectively. The DNA G+C content of genomic DNA was 70.7 mol%. Due to the differences in physiological, biochemical and genotypic data, CA1R205<sup>T</sup> could be discriminated from its closest neighbour. Thus, CA1R205<sup>T</sup> should be recognized as representing a novel species of the genus Streptomyces, for which the name Streptomyces coffeae sp. nov. is proposed. The type strain is CA1R205<sup>T</sup> (=TBRC 11244<sup>T</sup>=NBRC 114295<sup>T</sup>).