KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Settings

Author: search.filters.author.Niemhom, NantawanSubject: search.filters.subject.16S rRNA gene

Search Results

Now showing 1 - 2 of 2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Jiangella endophytica sp. nov., an endophytic actinomycete isolated from the rhizome of Kaempferia elegans
    (2019-02-01)
    Niemhom, Nantawan
    ;
    Chutrakul, Chanikul
    ;
    Suriyachadkun, Chanwit
    ;
    Tadtong, Sarin
    ;
    Thawai, Chitti
    An endophytic actinobacterium, designated strain KE2-3 <sup>T</sup> , was isolated from surface-sterilised rhizome of Kaempferia elegans. The polyphasic approach was used for evaluating the taxonomic position of this strain. The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of LL-diaminopimelic acid in the cell peptidoglycan, MK-9(H <inf>4</inf> ) as the major menaquinone, iso-C <inf>16: 0</inf> , anteiso-C <inf>15: 0</inf> , iso-C <inf>14: 0</inf> and iso-C <inf>15: 0</inf> as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes. Based on 16S rRNA gene sequence analysis, strain KE2-3 <sup>T</sup> was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357 <sup>T</sup> (99.3 %) followed by Jiangella alba DSM 45237 <sup>T</sup> (99.2 %), Jiangella alkaliphiliaDSM 45079 <sup>T</sup> (99.0 %), Jiangella gansuensis DSM 44835 <sup>T</sup> (98.8 %) and Jiangella mangrovi 3SM4-07 <sup>T</sup> (98.6 %). However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96%species circumscription threshold. The DNA G+C content of genomic DNA was 72.3 mol%. With the differences of physiological, biochemical and genotypic data, strain KE2-3 <sup>T</sup> could be discriminated from its closest neighbour. Thus, strain KE2-3 <sup>T</sup> should be recognised as a novel species of genus Jiangella, for which the name Jiangella endophytica sp. nov. is proposed. The type strain is KE2-3 <sup>T</sup> (=BCC 66359 <sup>T</sup> =NBRC 110004 <sup>T</sup> ).
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Herbidospora soli sp. Nov., isolated from soil
    (2018-01-01)
    Niemhom, Nantawan
    ;
    Thawai, Chitti
    A novel actinomycete strain PS42-9<sup>T</sup>, which formed short chains of spores borne on the tip of long sporophores arising from the substrate mycelium, was isolated from soil in Phu-Sang National Park, Phayao province, Thailand. The isolate contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars of strain PS42-9<sup>T</sup> were glucose, madurose, mannose, rhamnose and ribose. The characteristic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive glycophospholipids. The major menaquinone was MK-10(H<inf>4</inf>). The main cellular fatty acids were C<inf>17:</inf>1ω8c and C<inf>17: 0</inf>. The G+C content of the genomic DNA was 71.5 mol%. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain PS42-9<sup>T</sup> should be classified in the genus Herbidospora and was closely related to Herbidospora sakaeratensis DMKUA 205<sup>T</sup> (99.10 %) and Herbidospora yilanensis NBRC 106371<sup>T</sup> (98.61 %). The result of DNA–DNA hybridization and some physiological and biochemical properties indicated that strain PS42-9<sup>T</sup> could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Herbidospora soli sp. nov. is proposed. The type strain is PS42-9<sup>T</sup> (=BCC 46909<sup>T</sup>=NBRC 108780<sup>T</sup>).