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Author: search.filters.author.Panngeun, ThanapolHas files: No

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    Antioxidant Activity, γ-Aminobutyric Acid, and Genome Analysis of Lactiplantibacillus plantarum FL13-2 from Fermented Rice Flour
    (2025-09-01)
    Songsumanus, Apakorn
    ;
    Pinyosnit, Natawadee
    ;
    Kingkaew, Engkarat
    ;
    Panngeun, Thanapol
    ;
    Charunanthasunthon, Worasan
    Ten rod-shaped isolates of lactic acid bacteria (LAB) from the traditional fermented rice flour, khao-khab were identified as belonging to the genus Lactobacillus based on phenotypic characteristics. Group I isolates (FL12-1, FL18-1, FL19-1S, FL23-1, FL24-1, FL25-1, and FL26-1) were closely related to Limosilactobacillus fermentum, exhibiting 16S rRNA gene sequence similarity of 99.4%–100%. Group II included FL13-2 and FL22-2 (Group IIA) and FL17B (Group IIB), closely related to Lactiplantibacillus plantarum (99.9%–100%) and Lactiplantibacillus pentosus (100%), respectively. All isolates demonstrated strong antioxidant potential, with 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity—measured by the standard DPPH assay—ranging from 78.49±1.01% to 91.18±3.95%, surpassing that of ascorbic acid. Strain FL13-2, identified as L. plantarum through genomic analysis (Average Nucleotide Identity (ANI) 98.5%, digital DNA-DNA hybridization (dDDH) 92.5%), produced 2.43 g/L of γ-aminobutyric acid (GABA). It harbored key biosynthesis and transport genes (gadB, gadC, gatABCD, pdxK). Phylogenetic analysis confirmed high sequence similarity of these genes with other LAB strains, supporting their functional roles. The gad system was implicated in acid resistance and GABA production under low pH conditions. Genome annotation revealed bacteriocin genes (Enterocin X β, Plantaricin E, and F) and indicated low pathogenicity and absence of virulence factors. Antimicrobial resistance genes (vanH, vanT, vanY) were detected; however, these are intrinsic to LAB and associated with essential cell wall biosynthesis rather than acquired resistance. Additionally, the presence of the qacJ efflux pump and hlyIII gene—both commonly found in probiotic strains—further supports the safety profile of FL13-2. These findingshighlight L. plantarum FL13-2 as a promising multifunctional probiotic candidate with potent antioxidative, GABA-producing, and antimicrobial properties.
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    Cosmeceutical applications of the yeast beta-glucan and its beneficial skin effects
    (2024-09-16)
    Kingkaew, Engkarat
    ;
    Panngeun, Thanapol
    ;
    Jumi, Woratep
    ;
    Tanasupawat, Somboon
    The skin, our body's largest organ, comprises the epidermis, dermis, and subcutaneous tissue, playing a crucial role in protection and regulation. Beta-glucans (BG), derived from yeast by-products, exhibit cosmeceutical properties with applications in skincare. This chapter details the skin's structure and beta-glucans' molecular characteristics, emphasizing their solubility influenced by branching and polymerization. The beneficial features of BG for the skin, including antioxidant, immunomodulatory, and wound healing properties, are explored. In particular, BG's antioxidant capacity mitigates oxidative stress, while its immunomodulation aids in inflammation regulation and potential skin cancer prevention. The chapter also highlights BG's role in wound healing, tissue repair, and its anti-aging effects, demonstrating its versatility in skincare applications. Commercial products incorporating BG underscore its increasing presence in skincare formulations, aligning with consumer preferences for natural bioactive ingredients. Despite significant progress, further research is needed to explore BG's impact on complex skin systems and its potential in managing various skin conditions. The utilization of industrial by-products, such as spent yeast, as a sustainable source for BG extraction adds an eco-friendly dimension to its application in skincare formulations. Standardized extraction methods and deeper insights into BG interactions with different skin layers will contribute to unlocking its full potential in skincare.