KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Search Results

Now showing 1 - 7 of 7

Some of the metrics are blocked by your
consent settings
Analysis of secondary metabolites and genes related to the pathogenicity of the rice blast fungus Pyricularia oryzae
(2022-09-01)
Pradapphai, P.
;
Kumlung, T.
;
Parinthawong, N.
Pyricularia oryzae causes rice blast disease and is one of the most significant plant pathogens worldwide. The rice blast fungus can produce a variety of phytotoxic secondary metabolites and has been found to be essential for host invasion. Two isolates, virulent isolate RBR55003 and non-virulent isolate CCO56003 were selected which based on their abilities to cause blast symptoms on rice leaves. Pyriculol and picolinic acid were detected in culture filtrate of both isolates during the exponential growth phase. Expression of genes involved in fungal pathogenicity and plant defense were analyzed. The expression of MoPks19 gene in isolate RBR55003 was higher than avirulent isolate CCO56003, indicating that MoPks19 may play a role in the pathogenesis of RBR55003. The expression of the OsWRKY30 gene that plays an important role in the regulation of a defense-related gene in rice was higher in CCO56003 inoculated than in RBR55003 inoculated rice sample, indicating the increased resistance of rice plants occurring in rice after non-virulent isolate infection, while the expression of the OsCPS4 gene involved in phytoalexin synthesis in rice was not different after isolate infections. Gene expression analysis revealed different responses according to plant-pathogen relationships.
Some of the metrics are blocked by your
consent settings
Pathogenicity of Pyricularia oryzae on elite rice cultivars and geographical distribution of avirulence genes causing blast disease in Thailand
(2020-07-01)
Parinthawong, N.
;
Tansian, P.
Pyricularia oryzae is a plant pathogenic fungus causing rice blast disease. In Thailand, there is very little information about pathogenicity and avirulence gene of rice blast pathogen. Each of the 50 blast isolates obtained from blast disease samples collected from 19 provinces was investigated for avirulence gene on 31 near isogenic lines (NILs) and their pathotype on elite rice cultivars was determined using 24 Thai elite rice cultivars. Among the 50 isolates, Avr-Pita-2 was discovered mostly in isolates from the North (83%), Avr-Pikp and Avr-Pi7(t) were discovered in isolates from the North East (94%), while 8 avirulence genes including Avr-Pikp, Avr-Pikh, Avr-Pi1, Avr-Pi7(t), Avr-Piz, Avr-Pi12, Avr-Pi19 and Avr-Pi20 were discovered in isolates from the South (88%) and Avr-Pi9 was discovered in isolates from the Central region of Thailand (71%). Isolates BRM60001 that carried the most numbers of Avr gene was unable to infect any elite rice cultivars, while isolates that carried as less as 2 Avr genes could infect few elite rice cultivars. Isolate CRI59004.1 carried Avr-Pik (Avr-Pikh, Avr-Pikm, Avr-Pi7(t)), Avr-Pi9, Avr-Pish, Avr-Pib, Avr-Piz-5, Avr-Pita, Avr-Pita-2 and Avr-Pi19, could infect the greatest numbers of elite rice cultivars. The information regarding the distribution of Avr gene in P. oryzae will be useful for forecasting rice blast and disease resistance breeding programs.
Some of the metrics are blocked by your
consent settings
Investigation of rice blast resistant genes in Thai elite rice varieties (Oryza sativa L.) for improvement of broad-spectrum blast disease resistance variety
(2020-01-01)
Poonsin, R.
;
Parinthawong, N.
The broad-spectrum blast disease resistance rice Huai variety (GS19567) has been reported to contain more than one blast resistance genes which one of them was mapped on chromosome 11. The blast resistance genes was investigated using gene specific primers in Huai and other 40 Thai elite rice varieties. The results obtained from polymerase chain reaction revealed that all tested varieties contained at least one blast resistant gene. The most frequent finding resistant gene was Pib, while Pib was missing in Huai variety. Therefore, in order to enhance broad spectrum blast disease resistance in a sustaining blast resistance variety, the resistance gene Pib is recommened to be used as candidate gene in breeding program.
Some of the metrics are blocked by your
consent settings
Blast resistance evaluation and genetic inheritance of gene controlling leaf blast resistance in Dawk Pa-yawm Rai variety (GS23774)
(2020-01-01)
Janthasri, S.
;
Parinthawong, N.
The landrace rice Dawk Pa-yawm Rai variety was highly resistant to 18 blast isolates, moderately resistant to 5 and susceptible to 2 blast isolates. Therefore, Dawk Pa-yawm Rai variety was broad-spectrum resistance to many blast isolates and can be used as genetic resource in breeding programs for blast disease resistance. The genetic inheritance of blast resistance in F<inf>2</inf> population was identified. Seven hundred and thirty F<inf>2</inf> plants from a cross between KDML105 (recipient) and Dawk Pa-yawm Rai (donor) were generated. The disease assessment revealed 524 F<inf>2</inf> plants were resistant and 206 F<inf>2</inf> plants were susceptible. The segregation of resistance and susceptible phenotypes showed a goodness of fit to the ratio 3:1 (R:S). The result found is important factor and useful in mapping of blast resistant gene in Dawk Pa-yawm Rai variety.
Some of the metrics are blocked by your
consent settings
Classification and diversity assessment of Pyricularia oryzae based on leaf and neck blast pathogenesis on Khao Dawk Mali 105
(2020-01-01)
Pradapphai, P.
;
Parinthawong, N.
Forty isolates of P. oryzae were obtained from disease outbreak areas in various regions of Thailand. The fungi were divided into two groups; group 1 included 2 isolates which cause only neck blast with disease score of 7 – 9 and group 2 included 23 isolates which cause leaf and neck blast symptoms with disease scores of 5 – 6 and 7 – 9, respectively. The two groups of fungi were tested on 31 NILs. The avirulence genes including Avr-Pik (Avr-Pik, Avr-Pikm, Avr-Pikp), Avr-Pi1, Avr-Pi3, Avr-Pi5(t), Avr-Pi7(t), Avr-Pi9, Avr-Pi20, Avr-Pii and Avr-Pita were found in both groups whereas Avr-Pi11, Avr-Pi12, Avr-Pi19, Avr-Pia, Avr-Pib, Avr-Pikh, Avr-Piks, Avr-Pish, Avr-Pita, Avr-Piz, Avr-Piz-5 and Avr-Piz-t were detected only in the group 2. The results explained the relationship between fungi and the avirulence genes functioned at different stages of rice.
Some of the metrics are blocked by your
consent settings
Investigation of blast resistance genes in a broad-spectrum resistant indigenous rice, Yang Mawng variety
(2020-01-01)
Suksiri, W.
;
Parinthawong, N.
The indigenous rice Yang Mawng variety was highly resistant to 24 isolates and moderate resistant to 3 isolates of P. oryzae. The results confirmed the broad-spectrum resistance to blast disease of Yang Mawng variety. The pathogenicity test of 27 isolates of P. oryzae was conducted on 31 Near Isogenic Lines (NILs). The result showed that virulence index (VI) was valued from 0 to 0.50 and the most pathogenic isolate on NILs was UBN2010 13515. The investigation for 8 rice blast disease resistance genes of Yang Mawng variety was done using blast resistance gene specific primers and PCR technique. The result showed that 5 blast resistance genes including Pi9, Pigm(t), Pi54, Pib and Pita, were detectable. The information of this research is a preliminary data for study of resistance genes present in Yang Mawng variety.
Some of the metrics are blocked by your
consent settings
Mating type and genetic diversity analysis of Pyricularia oryzae collected from Thai rice varieties
(2018-12-01)
Tansian, P.
;
Parinthawong, N.
Rice blast disease caused by Pyricularia oryzae, is one of the most damaged of rice production worldwide. The fungi are a great diversity on both genotype and pathotype. The genetic relationship of P. oryzae collected in Thailand during year 2016 and 2017 was analyzed. Rice plants appeared blast symptom were collected from epidemic areas and was isolated by single spore. All isolates were identified for mating type using MAT1-1 and MAT1-2 primer sets. In this result, the mating type MAT1-2 was mostly found in Thailand and only about 7.7 % of MAT1-1 was found in the population. In this observation, both MAT1-1 and MAT1-2 were found from isolates collected from the same location in Phetchabun province. Therefore, the genetic analysis of 14 Magnaporthe grisea using microsatellite (MGM) markers was cluster-analyzed using UPGMA method of the SHAN program. The result showed cluster analysis was separated the population into 11 groups. Group 1 to 7 showed the percentage of the population with 3.8, 5.8, 36.5, 13.5, 3.8, 25.1 and 3.8, respectively. For group 8-11, the rice blast pathogen from North and South of Thailand were separated into single isolate group. Group 6 was the highest diversity of province numbers which obtained from diverse rice varieties that showed similar genotype but difference in pathogenicity. The difference isolates of fungi could infect the same rice variety. Genetic diversity and determination of mating type of rice blast populations in Thailand are needed to study and useful for predicting an epidemic of rice blast disease and selecting the appropriate isolates for blast breeding programs.

KMITL

Browse

Filters

Settings

Sort By

Results per page

Search Results