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Author: search.filters.author.Phasomkusolsil, SiripornSubject: search.filters.subject.Anopheles minimus

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    Insect repellent activity of medicinal plant oils against aedes aegypti (LINN.), anopheles minimus (Theobald) and culex quinquefasciatus say based on protection time and biting rate
    (2010-01-01)
    Phasomkusolsil, Siriporn
    ;
    Soonwera, Mayura
    This study investigated insect bite protection and length of the protection with 30 repellents which were divided into 3 categories: plant oil, essential oil and essential oil with ethyl alcohol, tested against three mosquito species, Aedes aegypti, Anopheles minimus and Culex quinquefasciatus, under laboratory conditions. The plant oil group was comprised of Phlai (Zingiber cassumunar) and Sweet basil (Ocimum basilicum). Both substances were effective as repellents and feeding deterrents against An. minimus (205 minutes protection time and a biting rate of 0.9%), Cx. quinquefasciatus (165 minutes protection time and 0.9% biting rate) and Ae. aegypti (90 minutes protection time and 0.8% biting rate). Essential oil from citronella grass (Cymbopogon nardus) exhibited protection against biting from all 3 mosquito species: for An. minimus, Cx. quinquefasciatus and Ae. aegypti, the results were 130 minutes and 0.9%, 140 minutes and 0.8%, and 115 minutes and 0.8%, respectively. The period of protection time against Ae. aegypti for all repellent candidates tested was lower than the Thai Industrial Standards Institute (TISI) determined time of greater than 2 hours.
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    Potential larvicidal and pupacidal activities of herbal essential oils against Culex quinquefasciatus say and Anopheles minimus (Theobald)
    (2010-01-01)
    Phasomkusolsil, Siriporn
    ;
    Soonwera, Mayura
    The larvicidal and pupacidal effects of eight herbal essential oils were tested against third instar (L<inf>3</inf>), fourth instar (L<inf>4</inf>), and pupal stages of Culex quinquefasciatus and Anopheles minimus. Probit analysis was used to analyze the data. The larval mortality was recorded at 1, 5, 10, 30, and 60 minutes, and 24 hours. Pupal mortality was also recorded at 24 hours intervals for 96 hours when completed mortality was achieved. Citronella grass oil #2 (Cymbopogon nardus) proved to have the greatest toxicity against 3<sup>rd</sup> instar Cx. quinquefasciatus and An. minimus with LT<inf>50</inf> at 1.2 and <0.2 minute, respectively. It exhibited a high level of effectiveness against 4<sup>th</sup> instar Cx. quinquefasciatus (LT<inf>50</inf> at 5.1 minutes) and An. minimus (LT<inf>50</inf> at 0.9 minute). Regarding pupacidal activity, Cx. Quinquefasciatus and An. minimus pupae were susceptible to Lemon grass oil (LT<inf>50</inf> at 1.2 hours) and Citronella grass oil #1 (LT<inf>50</inf> at 0.6 hour), respectively. These oils had larvicidal and pupacidal activities with 100% mortality against L<inf>3</inf>, L<inf>4</inf> and pupal stage Cx. quinquefasciatus at 10 minutes, 30 minutes and 24 hours. They also caused 100% mortality of An. minimus at 5 minutes (L<inf>3</inf>, L<inf>4</inf>), and 24 hours (pupal stage).