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Author: search.filters.author.Phasuk, KanchanaHas files: No

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    Rapid E. coli detection based on fluorescence technique
    (2017-12-19)
    Suwan, Supakorn
    ;
    Phasuk, Kanchana
    ;
    Chitsakul, Kitiphol
    ;
    Piyawattanametha, Wibool
    This research focuses on developing a prototype tool that is portable and develop method to detect Escherichia coli (E. coli) in water by measuring the light of fluorescence from an enzymatic reaction. The procedure can be done easily without need for laboratory professionals within 2 hours. Total coliforms can measure a minimum of at least 10 MPN/mL. This method is an alternative method to determine water quality. It can reduce the shipping water samples step to laboratories, reducing the time to wait for results. And reduces the cost of detecting E. coli, while the efficiency of this method is no different with the Most Probable Number method.
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    Evaluation of rapid detection system for Escherichia coli in water samples
    (2017-02-21)
    Pipitsombat, Chanikarn
    ;
    Phasuk, Kanchana
    ;
    Suwan, Supakorn
    ;
    Piyawattanametha, Wibool
    We demonstrated a fast detection platform for Escherichia coli (E. coli) in water. The system is based on a fluorescence detection technique by determining enzyme β-D-glucuronidase (GUD) activity and relating that activity to E. coli concentration. We found the portable E. coli detection platform is able to detect both the fluorescence 4-MU signal from the reaction of GUD assay from 0.01-1 μg/ml and to enumerate the number of E. coli in the range of 10<sup>5</sup>-10<sup>7</sup> CFU/ml. The required incubation time is in between 20-240 min. In addition, we can estimate the maximum of incubation time for detecting minimum of E. coli 1 CFU/ml from the equation to be 576 mins. Our preliminary study demonstrates that the system can be used to enumerate the number of E. coli in water on-site and requires less detection time than traditional E. coli methods.