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Author: search.filters.author.Phunpruch, SaranyaSubject: search.filters.subject.Tetraspora sp. CU2551

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    Enhanced hydrogen production by optimization of immobilized cells of the green alga Tetraspora sp. CU2551 grown under anaerobic condition
    (2018-04-01)
    Maswanna, Thanaporn
    ;
    Phunpruch, Saranya
    ;
    Lindblad, Peter
    ;
    Maneeruttanarungroj, Cherdsak
    The green alga Tetraspora sp. CU2551 has previously been identified and characterized as a photosynthetic microorganism with high potential for H<inf>2</inf> production. In the present study, cells of Tetraspora CU2551 were entrapped and immobilized in an alginate matrix with the aim to analyze the effect of cell stacking and a reduced exposure of O<inf>2</inf> to the cells. The results showed that the most favorable immobilization conditions were 4% (w/v) of final alginate concentration and a cell concentration of 0.125 mg cell dry wt/mL alginate with a bead diameter of 2.80–3.35 mm. The H<inf>2</inf> production yields increased when the immobilized cells were incubated in S-deprived medium and this could be repeated at least for 3 production times. Maximal total H<inf>2</inf> production reached 7.68 ± 0.88 mL H<inf>2</inf>/25 mL medium, corresponding to a rate of 1182.45 ± 24.40 nmol H<inf>2</inf>/h/mg DW. This production is about 6 times higher compared to by cells in suspension, 2–10 times higher when compared to by other green algae, and 10–50 times higher when comparing with cyanobacteria. Based on our observations, immobilized cells of Tetraspora CU2551 is considered a very promising biological system for significant photobiological H<inf>2</inf> production by a photosynthetic microorganism.
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    Effect of pH on Biohydrogen Production in Green Alga Tetraspora sp. CU2551
    (2017-01-01)
    Maneeruttanarungroj, Cherdsak
    ;
    Phunpruch, Saranya
    The green alga Tetraspora sp. CU2551 has previously been identified as a hydrogen producing organism. With the promising potential in a high H<inf>2</inf> production yield, we further studied the enhancement of the H<inf>2</inf> production capacity by a substrate-level control to the key enzyme, Hydrogenase. This enzyme catalyzes the formation of biohydrogen gas from protons and electrons. Here, we investigated the effect of pH on the production rate which directly affected the flow of proton flux to the hydrogenase enzyme. The result revealed that pH of culture TAP medium was simply controlled by the use of HCl/NaOH solution over the pH range of 6.5 - 9.0 without any effect on the growth of algal cells. The H<inf>2</inf> production was observed in all ranges of our focusing pH with comparable to the control condition (pH 7.0 of TAP medium). In our best condition, an increase of the H<inf>2</inf> yield about 24% was found in cells cultivated in TAP medium adjusted the pH to 6.5 compared to the control condition. Our finding confirms the successful biohydrogen production enhancement by a substrate-level control for the key hydrogenase enzyme.