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Author: search.filters.author.Pipatpanukul, Chinnawut

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    A multi-channel optical fibre-based smartphone spectrophotometer for measuring the spectra of LED colours
    (2021-07-01)
    Puttharugsa, Chokchai
    ;
    Srikhirin, Toemsak
    ;
    Pipatpanukul, Chinnawut
    ;
    Houngkamhang, Nongluck
    This paper demonstrates the use of a smartphone as a low-cost multi-channel optical fibre spectrophotometer suitable for physics laboratory classes. A custom-designed cradle and structure support were fabricated using 3D printing. The plastic optical fibres were arranged and inserted into the hole of the cradle to guide the light to the rear camera of the smartphone. Here, we describe the multi-channel optical fibre-based smartphone spectrophotometer for measuring the spectra of different LED colours.
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    Application of printable antibody ink for solid-phase immobilization of ABO antibody using photoactive hydrogel for surface plasmon resonance imaging
    (2020-10-01)
    Shrestha, Binit
    ;
    Pipatpanukul, Chinnawut
    ;
    Houngkamhang, Nongluck
    ;
    Brandstetter, Thomas
    ;
    Rühe, Jürgen
    Biochips for multiplex blood typing through surface plasmon resonance imaging (SPRi) readout have been previously reported based on arrays of red blood cell (RBC) specific ABO antibodies (Abs). Although conventional surfaces, such as CM-dextran, are robust and highly specific, they are time and labor intensive to generate and functionalize. This work investigates the fabrication parameters and performance of printable ink consisting of a photoactive hydrogel precursor and antibody mixture for the fabrication of SPRi array biochip. The solid-phase immobilization of commercially available murine IgM ABO Abs on a SPR sensor chip with a multiplex detection of ABO antigens present on the surface membrane of human RBCs is reported. A quick and simple solid phase immobilization of protein on a SPR sensor chip was attained using the photocrosslinking polymer and microarray printing platform. A high density immobilized probes is obtained which allows high sensitivity, specificity, and surface regeneration. A signal to noise ratio of 22:1 and 25:1 at 5% and 10% immobilized-Ab respectively, is obtained with 5% RBC concentration. The sensor surface can be successfully regenerated for repeated use using DI water. Without loss of function, the Ab-printed chips could be stored up to 3 weeks at 4 °C.
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    Demonstration of light absorption and light scattering using smartphones
    (2020-01-01)
    Malisorn, Khemchira
    ;
    Wicharn, Surawut
    ;
    Plaipichit, Suwan
    ;
    Pipatpanukul, Chinnawut
    ;
    Houngkamhang, Nongluck
    This paper demonstrates the use of smartphones in an experiment of light absorption and light scattering. The LED display and camera of the smartphone are used as the light source and as the detector, respectively. The color wheel is used to choose the color of the light source to be shone through the sample for analysis. The detector directly measures the intensity of the light that passes through the sample to study light absorption according to the Beer-Lambert law. On the other hand, to investigate the light scattering, the detector orthogonally measures the intensity of the scattered light from the sample. The results of the light absorption correspond to the Beer-Lambert law. The scattered light from the sample is be measured by a smartphone. The experiment is easy to set up, without the need for any further expensive apparatus. We expect that this experiment will be useful for physics teachers to demonstrate light absorption and light scattering in the classroom or in a physics laboratory.
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    Gold nanorod-optical fiber for sensing biomolecular interaction
    (2020-01-01)
    Chueaiarrom, Channarong
    ;
    Loedsapchinda, Naruenard
    ;
    Puttharugsa, Chokchai
    ;
    Pipatpanukul, Chinnawut
    ;
    Srikhirin, Toemsak
    The optical fiber deposits with gold nanorods was successfully accomplished as a biosensor device which sensing to the refractive index change on the surrounding surface. The principle is based on the localized surface plasmon resonance (LSPR) occurs on the metal surface of gold nanorods (AuNRs). The gold nanorods were coated on the glass core fiber surface through the covalent bond. The uncladded area fiber optic was incubated in 5% of (3-Mercaptopropyl) trimethoxysilane (MPTMS) in ethanol for overnight to alter the thiol group on the surface. The gold nanorods with aspect ratio ⁓2.035 was deposited on thiol surface by incubation the optical fiber in gold nanorod solution for 6 hr. The sensor provides the sensitivity of 4.94×104 a.u./RIU. Moreover, antibody for blood grouping was immobilized on the AuNRs-optical fiber to detect the red blood cells. The AuNRs-optical fiber can detect A antigen on the immobilized anti-A surface with Δ%T of 0.98 and can detect B-antigen on the immobilized anti-B surface with Δ%T of 1.78. This work possesses possibility of a simple AuNRs-optical fiber to detect the biomolecular interaction. The authors would like to thank Thailand Center of Excellence in Physics for financial support.