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Author: search.filters.author.Poeaim, A.Subject: search.filters.subject.Antioxidant

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    Phytochemical screening, antioxidant activity and total phenolic content of methanolic extract of Phak Wan Ton (Crotalaria medicaginea Lam.)
    (2023-01-01)
    Thuion, T.
    ;
    Poeaim, S.
    ;
    Poeaim, A.
    The chlorophyll content of the methanolic extract of Crotalaria medicaginea without chlorophyll removal ranged from 0.50±0.03 to 1.24±0.06 mg/g extract, and with chlorophyll removal ranged from 0.09±0.01 to 0.18±0.00 mg/g extract. The highest total phenolic content using the Folin-Ciocalteu method was presented in the leaves extract without chlorophyll removal as 48.33±0.51 mg GEA/g extract. Moreover, the leaves extract without chlorophyll removal showed the lowest antioxidant activity with 50% inhibitory concentration (IC<inf>50</inf>) in DPPH radical scavenging assay at 344.76 µg/ml. Besides, the root extract exhibited rich potential for gram-positive bacteria, including Bacillus cereus, Bacillus subtilis, Kocuria rhizophila, Staphylococcus aureus and Staphylococcus epidermidis. The overall results showed that the extracts without chlorophyll removal had more efficiency than those with chlorophyll removal. Hence, the phytochemical screening was investigated only in the methanolic extract without chlorophyll removal. All extracts found alkaloids and coumarins, while tannins were found only in roots and did not display saponins. The current study suggested that the extracts of C. medicaginea Lam. gave the potential source of natural bioactive compounds.
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    Efficiency of antioxidant and absorbent on browning and the optimal factors of plant regeneration from young seed of gluta usitata (217 Mae Ka) by Tissue Culture
    (2018-11-01)
    Rakrawee, R.
    ;
    Kittibanpacha, K.
    ;
    Chareonsap, P. P.
    ;
    Poeaim, A.
    Gluta usitata (Anacardiaceae) is a lacquer tree and create lacquer varnish can be utilized in many aspects especially art and culture. Recently, its harvest faced troublesome and yields of varnish tree are decreased. Consequently, the conservation of plant has reduced, and this plant has become extinct. The experiment was chosen to use plant tissue culture technique to preserve and increase this plant. The experiment was compared the effect of Woody Plant Medium (WPM) combined with different concentration of antioxidants were 2, 4, 6, 8 g/L Poly Vinyl Pyrrolidone (PVP), 20, 30, 40, 60 mg/L ascorbic acid or citric acid and absorbents of 1, 2, 3 g/L activated charcoal to reduce browning of phenolic compound in the explants. The results showed that 2 g/L activated charcoal gave the lowest of browning on seed and medium (about 0.9±0.2 points). The addition of 40 mg/L ascorbic acid was generated the highest of shoots (approximately 1.88±0.4 shoots/seed) after culture for 4 weeks. in vitro propagation of seed germination on WPM supplemented with 0.5, 1, 1.5, 2 and 3 mg/L 6-Benzyl amino purine (BAP), Thidiazuron (TDZ) and meta-Topolin (mT) 3% sucrose and 0.2% phytagel was efficiently resulted. Other result revealed that 1.5 mg/L BAP, 2 mg/L TDZ and 2 mg/L mT gave the highest seed germination at 1.6±0.2, 1.8±0.2 and 1.6±0.2 shoots/seed, respectively. The best of plant growth regulators for seed germination was 2 mg/L of TDZ after 6 weeks.