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Author: search.filters.author.Pongtongkam, P.Subject: search.filters.subject.Shoot induction

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    Plant regeneration of Bauhinia purpurea by tissue culture technique
    (2024-05-01)
    Saparam, W.
    ;
    Poeaim, A.
    ;
    Pongtongkam, P.
    ;
    Chareonsap, P. P.
    ;
    Poeaim, S.
    Results showed that the seed of Bauhinia purpurea germination was archived 100% from half-strength MS medium supplemented with 3.0 mg/L Gibberellic acids (GA3) and gave 71.137 mm for the highest seedling. The optimal condition of nodal explants sterilization was E5, which gave 92% of survival explants. For the shoot induction method, the results showed that 24.270 mm was the highest proliferation of shoots achieved from MS medium supplemented with 1.0 mg/L 6-benzylaminopurine (BAP). The highest number of roots was 2.867 roots, and the highest root length was 33.453 mm, obtained at half-strength MS medium complemented with 0.25 mg/L Indole-3-acetic acid (IAA). The 73.33% of plantlets were survived after being transferred to plastic pot.
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    Micropropagation of Ironwood (Xylia xylocarpa (Roxb.) Taub.) by tissue culture
    (2024-03-01)
    Panudom, S.
    ;
    Pongtongkam, P.
    ;
    Chareonsap, P. P.
    ;
    Poeaim, A.
    ;
    Poeaim, S.
    It was found that the sterilization procedure was highly effective, resulting in a 100% survival rate of Xylia xylocarpa (Roxb.). After sterilization, the seeds were cultured on a half-strength Murashige and Skoog (MS) medium without adding plant growth regulators (PGRs). A maximum germination percentage of 100% was achieved after 2 weeks of culture. 1.5 cm-sized node segments from both seeds and mother trees were used for shoot induction. These node segments were treated with the same sterilization conditions as the seeds but with the addition of 0.1% HgCl2. The node segments achieved a 90% survival rate despite the additional chemicals. The node segments from seeds were then cultured on a full-strength MS medium supplemented with 0.50 mg/l of BAP. The results showed a maximum number of shoots, reaching 4.10±1.20 per explant and a mean shoot length of 3.87±0.30 cm. The part of node segments derived from mother trees showed 3.50±1.27 shoots per explant, accompanied by a mean shoot length of 3.18±0.89 cm when using BAP concentration of 0.25 mg/l after 12 weeks of cultivation. The rooting medium contained half-strength MS medium supplemented with 0.75 mg/l of IBA. After 8 weeks of being cultured in the rooting medium, the shoots exhibited a maximum rooting percentage of about 80%. The technique provides a valuable method for rapidly propagating and conserving this plant species in a controlled and efficient manner.
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    In vitro propagation from nodal segments of Arachis glabrata cultivar Florigraze
    (2020-09-01)
    Pholjad, A.
    ;
    Pongtongkam, P.
    ;
    Arananant, J.
    ;
    Poeaim, A.
    Arachis glabrata Benth. of rhizome perennial peanut is used as a forage plants with higher nutrient value. The nodal segments were used to propagate in vitro by 0.2% of HgCl<inf>2</inf>plus antibiotic and cefotaxime and preservative for plant tissue culture media active (PPM) at 15 min. It was the most effective procedure for disinfecting plant parts (survival of 73.33%). Shoot induction used to the nodal segments of A. glabrata cultivar Florigraze were cultured on MS medium combination with plant growth regulators in cytokinin group at different concentrations 0.5, 0.75, 1, 2, 3 and 5 mg L<sup>-1</sup>of 6-benzylaminopurine (BAP), N6- furfuryladenine (Kn), meta-Topolin (mT) and Thidiazuron (TDZ). The maximum growth rate was 66.67% on 5 mg L<sup>-1</sup>of mT was the highest shoot length of 18.43 mm after 30 days. Root induction was achieved on MS medium supplemented with 2 mg L<sup>-1</sup>of α-Naphthaleneacetic acid (NAA) and 0.2% Activated Charcoal (AC) after 8-12 days cultured when compared with MS medium without plant growth regulators at the basal condition. Thereafter, the plantlets were transferred into the soil for acclimatization. It is the first report using the nodal segment in vitro propagation.