KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Settings

Author: search.filters.author.Pornpukdeewattana, SoisudaSubject: search.filters.subject.biomass

Search Results

Now showing 1 - 2 of 2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Valorizing Red Seaweed Spent Biomass into Reducing Sugars for β-Carotene Production by Rhodotorula paludigena
    (2026-05-01)
    Kongsinkaew, Chatchol
    ;
    Tangsattayatithan, Chutipol
    ;
    Chittapun, Supenya
    ;
    Phiphatbunyabhorn, Parivat
    ;
    Laemthong, Tunyaboon
    Seaweed bioactive extraction generates de-extracted residual solids that remain carbohydrate-rich but are often underutilized. This study developed an integrated valorization route for Gracilaria fisheri spent biomass to produce fermentable sugars for β-carotene production by Rhodotorula paludigena CM33. Reducing sugar production was optimized using response surface methodology (Box–Behnken design) by varying reaction time, sulfuric acid concentration, and biomass loading at 90 °C. The predicted optimum (47.39 min, 2.50% (w/v) H<inf>2</inf>SO<inf>4</inf>, and 7.13% (w/v) biomass) yielded 22.41 g/L reducing sugars and was validated experimentally at 22.22 ± 0.19 g/L, indicating that the model reliably predicted reducing sugar production. The optimized condition was scaled up in a 22 L bioreactor with sequential acid hydrolysis followed by enzyme-assisted hydrolysis, increasing reducing sugars from ~30 to ~40 g/L. FTIR and SEM analyses indicated progressive modification of the carbohydrate matrix across processing stages. Batch cultivation of R. paludigena on the hydrolysate showed that ammonium sulfate supplementation significantly increased biomass, whereas β-carotene titers were not significantly different. Repeated-batch operation on non-supplemented hydrolysate sustained production over four cycles with β-carotene titers of 13.75–17.27 mg/L, demonstrating the operational feasibility of the hydrolysate-based system. Overall, this work demonstrates a practical seaweed biorefinery approach to upgrade G. fisheri spent biomass into sugars and carotenoid-rich yeast biomass.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Enhancing Biomass and Lipid Production in Messastrum gracile Using Inorganic Carbon Substrates and Alternative Solvents for Lipid Extraction
    (2025-03-01)
    Pan-utai, Wanida
    ;
    Pornpukdeewattana, Soisuda
    ;
    Inrung, Wilasinee
    ;
    Thurakit, Theera
    ;
    Srinophakun, Penjit
    Microalgae show promise as a biomass and bioproduct for applications in various industries. The cultivation of microalgae plays a crucial role in optimizing biomass yield and bioproduct accumulation. The provision of inorganic carbon substrates substantially enhances microalgal growth and lipid biosynthesis, resulting in marked increases in the production of biofuels and other bioproducts. This study examined biomass and lipid accumulation in Messastrum gracile IFRPD 1061 under inorganic stress conditions, previously unreported. M. gracile IFRPD 1061 was subjected to varying conditions of inorganic carbon substrates, 1–3 g·L<sup>−1</sup> sodium carbonate and bicarbonate concentration, to enhance biomass and lipid accumulation. Optimal productivity levels were observed with sodium bicarbonate addition of 3 g·L<sup>−1</sup> and 1 g·L<sup>−1</sup> for biomass and lipids, resulting in productivities of 392.64 and 53.57 mg·L<sup>−1</sup>·d<sup>−1</sup>, respectively. Results underlined the effectiveness of sodium carbonate and bicarbonate as inorganic carbon sources for stimulating microalgal growth and enhancing the production of high-value products. The extraction of lipids from freeze-dried biomass of M. gracile IFRPD 1061 demonstrated optimal yield using methanol/hexane solvents compared with the control experiments. Lipid extraction yields using methanol/hexane were 42.18% and 46.81% from oven-dried and freeze-dried biomass, respectively. Lipids extracted from oven-dried M. gracile IFRPD 1061 using methanol/hexane/chloroform solvents indicated the potential of methanol/hexane as a solvent for lipid extraction from dry microalgal biomass using an ultrasonic-assisted technique. This study contributes valuable insights into maximizing biofuel and bioproduct production from microalgae, highlighting A. gracilis as a promising candidate for industrial applications.