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Author: search.filters.author.Pornsopin, N.

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    CHARACTERIZING THE DENSITY AND TYPE OF LEAF TRICHOMES IN TOMATO FOR RESISTANCE TO YELLOW LEAF CURL DISEASE
    (2025-10-01)
    Earsakul, S.
    ;
    Pornsopin, N.
    ;
    Techawongstien, S.
    ;
    Lapjit, C.
    ;
    Jeeatid, N.
    Global tomato (Solanum lycopersicum L.) production faces a major threat of tomato yellow leaf curl disease (TYLCD), transmitted by the whitefly (Bemisia tabaci). However, the resistance to TYLCD can gain enhancement by managing the vector. In tomatoes, the trichomes act as physical barriers against whiteflies. Developing tomato cultivars with specific trichome types in higher density works as a promising alternative to insecticides. The presented study investigated the relationship between trichome types, their density, and resistance to TYLCD by inoculating the cultivated tomatoes with the whitefly transmission method. The trichomes’ analysis also succeeded under a compound light microscope, aiding the selection of cultivars with high trichome density and TYLCD resistance. A negative correlation appeared between the glandular trichome type VI density and TYLC virus (TYLCV) resistance. Highly resistant tomato accessions (KKU-T23157, KKU-T23160, KKU-T23163, and KKU-T23164) and moderately resistant accessions (KKU-T23152, KKU-T23154, and KKU-T23172) displayed all five types of trichomes, with type VI density of 32–55/mm² and 32.67–42/mm², respectively. Susceptible tomato cultivars had only three trichome types, with type VI density of 9–19/mm². The presence of high glandular trichome type VI density, Ty-2, and Ty-3 resistance genes contributed to the TYLCD resistance. These selected tomato cultivars proved valuable for future tomato breeding programs.
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    Evaluation of chili (Capsicum annuum L.) progressive lines for resistance against Colletotrichum capsici and Colletotrichum gloeosporioides
    (2024-09-01)
    Subpang, N.
    ;
    Pornsopin, N.
    ;
    Earsakul, S.
    ;
    Techawongstien, S.
    ;
    Falab, S.
    Anthracnose (Colletotrichum spp.) is a devastating disease that affects the production of chili all over the world. Colletotrichum spp. with strong pathogenicity include C. capsici and C. gloeosporioides, which can infect both immature and mature pepper fruit and cause significant economic losses for the chili industry. The objective of this study was to evaluate the progressive lines of chili for resistance to anthracnose disease. The experiments were carried out by a completely random design (CRD) with three replications and five fruits per replication. In this experiment, 30 progressive lines of chili with different resistant genetic sources (PBC932 and PBC80) and one susceptible cultivar were inoculated with C. capsici and C. gloeosporioides into the two chili fruit stages (green and ripe mature) by microinjection technique. The disease severity was evaluated by lesion diameter seven days after inoculation. Based on their resistance level to two Colletotrichum species at the two fruit stages, the result showed that all progressive lines were classified into eight groups. Five progressive lines, i.e., AT1-1, AT2-3, AT3-1, AT5-2, and AT6-1, were highly resistant (HR) to two Colletotrichum species at both mature fruit stages. Therefore, five progressive lines should be used as high potential lines for improving new varieties with high resistance to anthracnose disease.
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    Evaluation of processing tomato resistance to tomato yellow leaf curl virus (TYLCV) and detecting Ty-2 and Ty-3 resistance genes with SCAR-markers
    (2024-09-01)
    Earsakul, S.
    ;
    Pornsopin, N.
    ;
    Techawongstien, S.
    ;
    Lapjit, C.
    ;
    Jeeatid, N.
    Tomato yellow leaf curl virus (TYLCV) is the major yield losing viral disease in both the vegetative and reproductive stages of tomato (Solanum lycopersicum L.) production worldwide. The objective of this study was to evaluate the tomato for resistance to TYLCV and good agronomic traits. In this experiment, yield and yield components of 25 accessions were evaluated in field conditions and compared with one resistant and one susceptible cultivar during the dry season (October 2021-February 2022). The randomized complete block design with three replications and 10 plants of each was designed. The plant was inoculated with TYLCV by whitefly transmission, and the resistance genes (Ty-2 and Ty-3) were examined using two molecular markers. The result showed that three tomato accessions, i.e., KKU-T23157, KKU-T23160, and KKUT23164 were discovered to share the same resistance gene (Ty-2 and Ty-3) as the resistance check cultivar and showed disease responses ranging from resistance (R) to high resistance (HR). Moreover, all three accessions (KKU-T23157, KKU-T23160, and KKU-T23164) showed high yields and yield components, i.e., yield (3.18, 3.74, and 3.38 kg plant<sup>-1</sup>), fruit weight (137.14, 126.71, and 136.91 g fruit<sup>-1</sup>), firmness (5.00, 5.00, and 0.49 N), and the flesh thickness (8.53, 8.62, and 5.96 mm), respectively. This information will offer a supportive direction for tomato breeders to develop TYLCV-resistant cultivars.
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