KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Settings

Author: search.filters.author.Pradapphai, P.Subject: search.filters.subject.Pyricularia oryzae

Search Results

Now showing 1 - 2 of 2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Analysis of secondary metabolites and genes related to the pathogenicity of the rice blast fungus Pyricularia oryzae
    (2022-09-01)
    Pradapphai, P.
    ;
    Kumlung, T.
    ;
    Parinthawong, N.
    Pyricularia oryzae causes rice blast disease and is one of the most significant plant pathogens worldwide. The rice blast fungus can produce a variety of phytotoxic secondary metabolites and has been found to be essential for host invasion. Two isolates, virulent isolate RBR55003 and non-virulent isolate CCO56003 were selected which based on their abilities to cause blast symptoms on rice leaves. Pyriculol and picolinic acid were detected in culture filtrate of both isolates during the exponential growth phase. Expression of genes involved in fungal pathogenicity and plant defense were analyzed. The expression of MoPks19 gene in isolate RBR55003 was higher than avirulent isolate CCO56003, indicating that MoPks19 may play a role in the pathogenesis of RBR55003. The expression of the OsWRKY30 gene that plays an important role in the regulation of a defense-related gene in rice was higher in CCO56003 inoculated than in RBR55003 inoculated rice sample, indicating the increased resistance of rice plants occurring in rice after non-virulent isolate infection, while the expression of the OsCPS4 gene involved in phytoalexin synthesis in rice was not different after isolate infections. Gene expression analysis revealed different responses according to plant-pathogen relationships.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Classification and diversity assessment of Pyricularia oryzae based on leaf and neck blast pathogenesis on Khao Dawk Mali 105
    (2020-01-01)
    Pradapphai, P.
    ;
    Parinthawong, N.
    Forty isolates of P. oryzae were obtained from disease outbreak areas in various regions of Thailand. The fungi were divided into two groups; group 1 included 2 isolates which cause only neck blast with disease score of 7 – 9 and group 2 included 23 isolates which cause leaf and neck blast symptoms with disease scores of 5 – 6 and 7 – 9, respectively. The two groups of fungi were tested on 31 NILs. The avirulence genes including Avr-Pik (Avr-Pik, Avr-Pikm, Avr-Pikp), Avr-Pi1, Avr-Pi3, Avr-Pi5(t), Avr-Pi7(t), Avr-Pi9, Avr-Pi20, Avr-Pii and Avr-Pita were found in both groups whereas Avr-Pi11, Avr-Pi12, Avr-Pi19, Avr-Pia, Avr-Pib, Avr-Pikh, Avr-Piks, Avr-Pish, Avr-Pita, Avr-Piz, Avr-Piz-5 and Avr-Piz-t were detected only in the group 2. The results explained the relationship between fungi and the avirulence genes functioned at different stages of rice.