The antagonistic potential of Neosartorya and Talaromyces proved to control leaf spot of kale caused by Alternaria brassicicola. Leaf spot symptoms were collected from Nakornpathom, Suphanburi, Ratchaburi and Nonthaburi provinces, isolated and identified the pthogen by morphological and molecular characterization. Four isolates were confirmed as A. brassicicola. The pathogenicity test was done by detached leaf bioassay and found that AbK-NP01 was the highest virulence pathogenic isolate. Dual culture technique was used to assay with eight species of antagonistic fungi (N. hiratsukae, N. pseudofischeri, N. aureola, N. spinosa, N. fennelliae, Neosartorya sp., T. trachyspermus and T. muroii) against A. brassicicola. The best effective of antagonist to control the tested pathogen was N. spinosa CHA09-A01.
