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Item type:Publication, Formulation Development of Serum Beads: Investigating the Influence of Sodium Alginate/Iota Carrageenan Ratio on Bead Characteristics(2024-05-01) ;Woraphokanunt, Yada ;Teeranachaideekul, Veerawat ;Rittiboon, AreeJatupornpipat, MarisaBackground and Objectives: To develop cosmetic products by using Ionic gelation techniques as the main technique in the production of serum beads. The purpose of this study is to develop the serum beads recipe by studying the ratio of various types of gelling agents for complete serum beads products. Methodology: Test the ratio of gelling agents in serums that create beads with ionic gelation techniques by Sodium Alginate and Iota-carrageenan which affect the appearance and beads texture after forming. Then compare the concentration of the gelling agent used in the suspension of serum beads that affects the ability to suspend serum beads. In this study, Carbopol® ultrez 20 was selected as the type of gelling agent. Main Results: The most suitable ratio for forming serum beads between Sodium Alginate and Iota-carrageenan is 0.20:0.30. Because the appearance of serum beads is spherical which can be pressed, cracked and spread on the surface easily-moderately, which is the most suitable characteristic for serum beads compared with the other ratios. When the serum beads were suspended inside the Carbopol® ultrez 20 gel at different concentrations and sensory tests, it was found that Carbopol® ultrez 20 at a concentration of 0.70 percent was the most favored. After storing in accelerated conditions to test the stability of the product, it was found that all concentrations of the gelling agent Carbopol® ultrez 20 could make the serum beads suspended and disperse inside the gel, but the viscosity was significantly reduced in each formulation. Conclusions: Conclusion: The ratio of Sodium Alginate and Iota-carrageenan affects the formation and hardness of beads. The most suitable ratio in this study is 0.20:0.30 and the gelling agent Carbopol® ultrez 20 at a concentration of 0. 70 percent is the most suitable choice for using as a suspension gel in the serum bead cosmetic formula. Because it is the most stable and has the least change when stored in accelerated condition for 8 weeks. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Optimization of the phosphate-solubilizing fungus, Aspergillus japonicus SA22P3406, in solid-state cultivation by response surface methodology(2009-12-01) ;Nopparat, Chalit ;Jatupornpipat, MarisaRittiboon, AreePhosphorus (P) is one of the essential macronutrients for plant growth and reproduction. Plants acquire P from the soil solution as phosphate anions. However, phosphate anions are extremely reactive and may be immobilized through precipitation with cations such as Ca<sup>2+</sup>, Mg<sup>2+</sup>, Fe <sup>3+</sup> and Al<sup>3+</sup>, depending on the particular properties of the soil and as a result, the phosphate is highly insoluble and unavailable to plants. Application of phosphate-solubilizing fungi (PSF) has been added as fertilizer to increase P uptake and plant growth. The aim of this study was to use response surface methodology to determine the optimal factors of the phosphate-solubilizing fungus Aspergillus japonicus SA22P3406 for use in solid state cultivation, specifically the pH, temperature and ratio of water to dry rice bran (RWB). A. japonicus SA22P3406 was screened from 24 soil samples. Primary screening was carried out by a 10-fold serial dilution of the soil samples at 10<sup>-4</sup> to 10<sup>-6</sup> and then spread on Pikovskaya agar supplemented with 1.0% w/v of tricalcium phosphate at 37°C for 3-5 d. One hundred and fifty five fungal isolates produced high ratios of clear zone to colony on Pikovskaya agar supplemented with 0.003% w/v of rose bengal at 37°C for 7 d. The ratios were calculated by dividing the area of the clear zone by the area of the colony. Secondary screening was performed by measuring the available phosphate in liquid medium from the 62 fungal strains by CRD and DMRT at 5% probability. Two fungal strains, A. niger SA07P3332 and A. japonicus SA22P3406, were selected as most suitable for solubilizing tricalcium phosphate and producing the highest available phosphate levels of 3.01 and 2.99 mg P <inf>2</inf>O<inf>5</inf> ml<sup>-1</sup>, respectively. A. japonicus SA22P3406 was cultivated in 5.0 g rice bran to study the optimal conditions using response surface methodology with a central composite design. The optimum conditions of pH, temperature and RWB were 7.37, 36.0°C and 82.10% v/w of dry rice bran, respectively. These conditions were used in the solid-state cultivation and a sporulation curve was determined. The fungal sporulation had a lower than average decadic logarithm of practical quantitative sporulation of 8.46 compared with the estimated quantitative sporulation of 8.53 at the 5th day of incubation.
