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Author: search.filters.author.Ruamrungsri, S.Has files: No

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    Induced mutation of curcuma hybrid cv. sweetmemory through tissue culture by ethyl methanesulphonate (EMS)
    (2025-01-01)
    Yoosumran, V.
    ;
    Saetiew, K.
    ;
    Ruamrungsri, S.
    ;
    Akarapisarn, A.
    ;
    Teerarak, M.
    The ethyl methanesulphonate (EMS) is induced mutation in Curcuma hybrid cv. sweetmemory. The shoot size 1 cm were cultured on Murashige and Skoog medium (MS) supplemented with 2 mg/l BA for plantlet induction. After 8 weeks of culture, it was observed that the shoots soaked in 0% EMS at 60 and 120 minutes survived 100%. In additions it was found the highest percentage of shoot emergence, number of shoots, and plant height. While plants treated with EMS solution had decreased survival and growth rates. Thereafter, existing plantlets were potted, and growth was observed. It turned out that the plants none receiving EMS solution (control) had the survival rate and growth rate were better than those of the plants receiving EMS solution. Results showed that control plants receiving 0% EMS at 60 minutes gave highest content of chlorophyll a as 4.06 µg/cm<sup>2</sup>, chlorophyll b of 2.08 µg/cm<sup>2</sup> and carotenoid of 3.09 µg/cm<sup>2</sup> but there was none statistical difference with the plants that received EMS solution. The control plants had larger stomata sizes than those that received the EMS solution. However, there was no difference in the number of chloroplasts among treatments. The living plantlets from all treatments were tested Random amplified polymorphic DNA (RAPD) marker and genomic DNA was extracted from fresh leaves. A dendrogram was constructed polymorphic bands using the NTSYSpc program (version 2.10p) showing 2 clusters which separated with similarity coefficients ranging from 0.74-1.00.
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    Micropropagation of young inflorescence Curcuma hybrid In vitro
    (2022-05-01)
    Yoosumran, V.
    ;
    Saetiew, K.
    ;
    Ruamrungsri, S.
    ;
    Akarapisarn, A.
    ;
    Teerarak, M.
    Five cultivars of curcuma hybrid consisted of 3 Paracurcuma and 2 Eucurcuma young inflorescences were cultured on MS medium supplemented with 0, 0.5 and 1 mg/l NAA and BA for 8 weeks. It was found that young inflorescences Paracurcuma hybrid Curcuma ‘Yuki’ cultured on MS medium supplement with the 1 mg/l NAA and 0.5 mg/l BA were able to induce the most shoots at 2.67 shoots, with the highest percentage of shoots (80.00%) and the average plant height is 5 cm. For Curcuma ‘Burgundy’ and Curcuma ‘Phonphisit’ cultured on MS medium supplement with 1 mg/l NAA and 1 mg/l BA, they were able to induce the most shoots at 1.66 and 1.78 shoots, the highest percentage of shoots is 73.33 and 76.67% respectively and the average plant height is 5 cm. The other cultivars Eucurcuma consist of Curcuma ‘Sweetmemory’ and Curcuma ‘Banrai Red’. The young inflorescences were divided into 2 parts, the upper and the lower one. The result shows that the lower shoot induction is better than upper one. Curcuma ‘Sweetmemory’ grown on MS medium supplemented with 0.5 mg/l NAA and 0.5 mg/l BA were able to induce the most shoots at 2.38 shoots, and the highest percentage of shoots is 83.33%. Curcuma ‘Banrai Red’ grown on MS medium supplemented with 0.5 mg/l NAA and 1 mg/l BA were able to induce the most shoots at 1.98 shoots, the highest percentage of shoots is 63.33% and both cultivars were the same height average at 5 cm.
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    Improvement of Dendranthemum grandiflora cv. canter with colchicine in vitro
    (2020-01-01)
    Asoko, N.
    ;
    Ruamrungsri, S.
    ;
    Yoosumran, V.
    ;
    Saetiew, K.
    The mutation of chrysanthemum with ray florets was induced by colchicine. The explants were cultured on Murashige and Skoog (MS) medium supplemented with 2 mg/l NAA and 4 mg/l Kinetin for inducing calli for 12 weeks. The shoots were regenerated from callus when cultured on MS medium for 8 weeks. The plant growth, size of stomata and number of chloroplasts per stomata were not statistically different in each treatment. However, the ray florets were received high levels of colchicine is less to survivor rate. The plantlet of chrysanthemum was transfered to pot plant containing soil and chopped coconut. Five of ten plant showed the flowers color changed from purple into a pinkish-orange at the 0.10% colchicine for 12 hours. The explant obtained from soaked of 0.15 % colchicine for 12 hours with the highest number of flowers per plant, 72 flowers per plant.
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    Induced mutation of dendranthemum grandiflora through tissue culture by Ethyl Methanesulphonate (EMS)
    (2018-01-01)
    Yoosumran, V.
    ;
    Ruamrungsri, S.
    ;
    Duangkongsan, W.
    ;
    Kanjana, S.
    The induced mutation by ethyl methasulphonate (EMS) was used for new cultivar of chrysanthemum. The calli were regenerated from immature petals of chrysanthemum (Dendranthemum grandiflora) 'vivic'. The size of 0.5x.05 cm ray florets were cultured on Murashige and Skoog medium (MS) supplemented with 2 mg/l NAA and 4 mg/l Kinetin for inducing calli. The calli were soaked in 0, 0.5, 1.0, 1.5 and 2% EMS for 06 and 026 minutes to induce mutation. Afterwards, they were cultured in MS medium supplemented with 2 mg/l NAA and 4 mg/l Kinetin to induce shoots for 4 weeks. The LD50 were 1.22% EMS for 60 min and 0.72% EMS for 120 min. The shoots were regenerated from callus in control 76.67% but they could not regenerate in the EMS concentration which is higher than 1.5 mg/l. After that they were transferred to the new bottles and cultured with the same medium for every 4 weeks. All shoots regenerated the roots in the MS medium without the plant growth regulator. Rooting plants were transferd to the soil pot plants. The EMS has the efficiency to induce the in vitro mutation. There are 3 different mutations characteristic which were found in this experiment. The first were obtained in 0.5% EMS for 60 min which resulted in white stacked ray florets with 2 layers, green disk florets. The second mutation characteristic were found in 1% EMS for 60 min which the flower turned into yellow petal stacked with 2 layers, green disk florets and also the white and yellow ray florets. The last mutation characteristic of 0.5% EMS for 020 min made the ray florets slender white stacked with 2 layers and green disk florets.