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Author: search.filters.author.Ruangsomboon, SuneeratSubject: search.filters.subject.Carbohydrate

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    Hydrocarbon production and biodiesel properties of a green microalga Botryococcus braunii KMITL 2 cultivated outdoor in open pond and closed photobioreactor
    (2018-03-01)
    Ruangsomboon, Suneerat
    Botryococcus braunii is a promising feedstock for biodiesel production. In general, selection of the most suitable cultivation systems is based on profitable yield and oil quality. The feasibility of using this alga as feedstock for commercial biodiesel production was tested by comparing the cultivation in open pond and closed photobioreactor under the 5 g⋅kg<sup>-1</sup> optimum salinity condition previously found, and the results were that the hydrocarbon content (40.7±1.6%) of the alga cultivated in open pond was higher than that cultivated in the photobioreactor and its biomass (2.9±0.1 g⋅L<sup>-1</sup>) was much higher; its biodiesel properties, however, were nearly identical with cetane values of 48.15-56.99. Drastically different though were the hydrocarbon yields-cultivation in the open pond produced 2.3 times higher yield than the closed photobioreactor. Hence, outdoor cultivation under optimum salinity in open pond was considered suitable for biodiesel production by this algal strain.
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    Enhanced hydrocarbon production and improved biodiesel qualities of Botryococcus braunii KMITL 5 by vitamins thiamine, biotin and cobalamin supplementation
    (2018-01-01)
    Ruangsomboon, Suneerat
    ;
    Sornchai, Piyanuch
    ;
    Prachom, Noratat
    Botryococcus braunii, a green microalga which is a potentially good biodiesel feedstock, was isolated from a freshwater fish pond in Bangkok, and the effects of B-vitamins—thiamine, biotin and cobalamin as well as their mixes—on its biomass, hydrocarbon production, carbohydrate content and biodiesel properties were investigated, using a control group that was not supplemented with any vitamins. The addition of thiamine increased the alga's biomass and hydrocarbon content, whereas addition of biotin, cobalamin and mixes of all three vitamins did not increase its hydrocarbon content. Among the vitamins tested, thiamine 150 μg L<sup>− 1</sup> was the best for producing high biomass (2.08 ± 0.07 g L<sup>− 1</sup>), hydrocarbon content (37.29 ± 0.41%), hydrocarbon yield (0.77 ± 0.03 g L<sup>− 1</sup>) and hydrocarbon productivity (90.32 ± 3.05 mg L<sup>− 1</sup> d<sup>− 1</sup>). Maximum carbohydrate content (386 ± 39 mg g<sup>− 1</sup>) was achieved by addition of 1 μg L<sup>− 1</sup> cobalamin. Compared to the control group, the alga cultured in media supplemented with 100 μg L<sup>− 1</sup> thiamine showed better biodiesel properties with lower iodine value (76.42 g I<inf>2</inf> 100 g<sup>− 1</sup>), cold filter plugging point (9.90 °C) and higher cetane number (54.30). Therefore, thiamine was shown to be a promising culture medium supplement for this strain for using it as a feedstock for biodiesel production. An investigation of vitamin B-related gene in B. braunii KMITL 5 found related genes for thiamine (thi), biotin (bioB and bioF) and cobalamin (metE and metH) vitamins, which are marks of vitamin autotrophy in algae.
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    Enhanced growth and hydrocarbon production of Botryococcus braunii KMITL 2 by optimum carbon dioxide concentration and concentration-dependent effects on its biochemical composition and biodiesel properties
    (2017-01-01)
    Ruangsomboon, Suneerat
    ;
    Prachom, Noratat
    ;
    Sornchai, Piyanast
    The purposes of this study were to find the optimum level of supplementing CO<inf>2</inf> for increasing the biomass and hydrocarbon production of B. braunii KMITL 2 (A race) and to determine the effects of CO<inf>2</inf> level on CO<inf>2</inf> fixation and biodiesel properties. The experimental results showed that the alga supplemented with 10% CO<inf>2</inf> produced the highest biomass (1.48 ± 0.02 g L<sup>−1</sup>) and CO<inf>2</inf> fixation rate (100.43 ± 1.42 mg L<sup>−1</sup> d<sup>−1</sup>) that were 2.7 and 5.3 times higher than the control (0.04% CO<inf>2</inf>). The gravimetric hydrocarbon content (36.82 ± 3.39%) and hydrocarbon titer (0.35 ± 0.03 g L<sup>−1</sup>) of the alga supplemented with 5% CO<inf>2</inf> were 1.6 and 2.7 times higher than the control. Cultivation of this strain under 5% CO<inf>2</inf> gave the highest hydrocarbon yield and good biodiesel properties with the lowest iodine value and a higher CN value than the ASTM D6751 and EN 14214 fuel standards.