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Author: search.filters.author.Ruangsomboon, SuneeratSubject: search.filters.subject.Fatty acid

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    Enhancing biomass, hydrocarbon and biodiesel properties of green microalga Botryococcus braunii KMITL through gamma and UV radiation exposure
    (2024-12-01)
    Jongput, Buppha
    ;
    Chiwpreecha, Pattanasak
    ;
    Ruangsomboon, Suneerat
    ;
    Tongsri, Pajongjit
    This study investigated the effects of gamma (<sup>137</sup>Cs, 0–250 Gy) and UV (UV-C, 0–12 h) radiation on growth and biodiesel properties of Botryococcus braunii KMITL. For gamma radiation, maximum biomass (1.37 ± 0.02 g L<sup>−1</sup>) was achieved with 50 Gy, while a dose of 200 Gy resulted in the highest hydrocarbon content (51.84 ± 0.20%) and yield (0.66 ± 0.01 g L<sup>−1</sup>). For UV radiation, a 9 h exposure produced the highest biomass (2.45 ± 0.05 g L<sup>−1</sup>), hydrocarbon content (55.01 ± 1.22%), and yield (1.35 ± 0.04 g L<sup>−1</sup>). Algae exposed to gamma radiation within the range of 0–150 Gy exhibited C16:0 as the dominant fatty acid methyl ester (FAME), similar to those exposed to UV radiation, while algae exposed to 200–250 Gy displayed C18:1n9t as the dominant FAME. High levels of gamma and UV radiation were observed to lengthen fatty acid chains and increase unsaturated fatty acids. The cetane values of biodiesel from algae exposed to gamma and UV radiation ranged from 64.55 ± 0.14–66.47 ± 0.20 and 59.43 ± 0.04–65.27 ± 0.22, respectively, all meeting standard criteria. Both gamma and UV radiation also improved the saponification value and cold flow properties of the biodiesel. These findings suggest that controlled levels of gamma and UV radiation effectively enhance hydrocarbon yields with significant implications for biofuel production.
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    Hydrocarbon production and biodiesel properties of a green microalga Botryococcus braunii KMITL 2 cultivated outdoor in open pond and closed photobioreactor
    (2018-03-01)
    Ruangsomboon, Suneerat
    Botryococcus braunii is a promising feedstock for biodiesel production. In general, selection of the most suitable cultivation systems is based on profitable yield and oil quality. The feasibility of using this alga as feedstock for commercial biodiesel production was tested by comparing the cultivation in open pond and closed photobioreactor under the 5 g⋅kg<sup>-1</sup> optimum salinity condition previously found, and the results were that the hydrocarbon content (40.7±1.6%) of the alga cultivated in open pond was higher than that cultivated in the photobioreactor and its biomass (2.9±0.1 g⋅L<sup>-1</sup>) was much higher; its biodiesel properties, however, were nearly identical with cetane values of 48.15-56.99. Drastically different though were the hydrocarbon yields-cultivation in the open pond produced 2.3 times higher yield than the closed photobioreactor. Hence, outdoor cultivation under optimum salinity in open pond was considered suitable for biodiesel production by this algal strain.
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    Effects of different media and nitrogen sources and levels on growth and lipid of green microalga Botryococcus braunii KMITL and its biodiesel properties based on fatty acid composition
    (2015-06-08)
    Ruangsomboon, Suneerat
    This work aimed to find an optimum culture medium for green microalga Botryococcus braunii KMITL and investigate its biodiesel properties based on fatty acid composition. Four different media were tested. Chlorella medium was the best medium for lipid yield. Among four nitrogen sources tested, KNO<inf>3</inf> produced the highest lipid yield. When varied the nitrogen concentrations, this strain gave the highest lipid yield at the highest nitrogen level. When cultivated in the best medium and nitrogen source and level for 30 days, and then cultivated further for 14 days in the medium with no nitrogen, the highest lipid content and yield were 49.94 ± 0.82% and 2.71 ± 0.02 g L<sup>-1</sup>, respectively. C16:0 fatty acid was the major fatty acid found. Fatty acid profiles of B. braunii KMITL cultivated in Chlorella medium with 1.25 g L<sup>-1</sup> KNO<inf>3</inf> gave the best biodiesel properties with the lowest iodine value, maximum cetane number, and lowest degree of unsaturation.