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Hydrocarbon production and biodiesel properties of a green microalga Botryococcus braunii KMITL 2 cultivated outdoor in open pond and closed photobioreactor
(2018-03-01)
Ruangsomboon, Suneerat
Botryococcus braunii is a promising feedstock for biodiesel production. In general, selection of the most suitable cultivation systems is based on profitable yield and oil quality. The feasibility of using this alga as feedstock for commercial biodiesel production was tested by comparing the cultivation in open pond and closed photobioreactor under the 5 g⋅kg-1 optimum salinity condition previously found, and the results were that the hydrocarbon content (40.7±1.6%) of the alga cultivated in open pond was higher than that cultivated in the photobioreactor and its biomass (2.9±0.1 g⋅L-1) was much higher; its biodiesel properties, however, were nearly identical with cetane values of 48.15-56.99. Drastically different though were the hydrocarbon yields-cultivation in the open pond produced 2.3 times higher yield than the closed photobioreactor. Hence, outdoor cultivation under optimum salinity in open pond was considered suitable for biodiesel production by this algal strain.
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Enhanced growth and hydrocarbon production of Botryococcus braunii KMITL 2 by optimum carbon dioxide concentration and concentration-dependent effects on its biochemical composition and biodiesel properties
(2017-01-01)
Ruangsomboon, Suneerat
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Prachom, Noratat
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Sornchai, Piyanast
The purposes of this study were to find the optimum level of supplementing CO<inf>2</inf> for increasing the biomass and hydrocarbon production of B. braunii KMITL 2 (A race) and to determine the effects of CO<inf>2</inf> level on CO<inf>2</inf> fixation and biodiesel properties. The experimental results showed that the alga supplemented with 10% CO<inf>2</inf> produced the highest biomass (1.48 ± 0.02 g L−1) and CO<inf>2</inf> fixation rate (100.43 ± 1.42 mg L−1 d−1) that were 2.7 and 5.3 times higher than the control (0.04% CO<inf>2</inf>). The gravimetric hydrocarbon content (36.82 ± 3.39%) and hydrocarbon titer (0.35 ± 0.03 g L−1) of the alga supplemented with 5% CO<inf>2</inf> were 1.6 and 2.7 times higher than the control. Cultivation of this strain under 5% CO<inf>2</inf> gave the highest hydrocarbon yield and good biodiesel properties with the lowest iodine value and a higher CN value than the ASTM D6751 and EN 14214 fuel standards.
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Effects of low pH and Pb2+ stress on living cyanobacterium, Phormidium angustissimum West & G.S.West: A test of its feasibility as a living biosorbent
(2013-06-01)
Ruangsomboon, Suneerat
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Wongrat, Ladda
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Choochote, Sakchai
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Ganmanee, Monthon
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Saparnklang, Atiya
Pb2+ adsorption by the living cyanobacterium, Phormidium angustissimum followed the Langmuir adsorption model, with the maximum adsorption capacity (q <inf>max</inf>) of 295.4 ± 13.8 mg g-1. This result suggests that P. angustissimum is a promising living biosorbent to remove Pb2+ from wastewaters. Living biosorbents are better able to remove Pb2+ from wastewater than dead biosorbents, however there are practical limitations for their use are encountered in extreme conditions such as low pH and high Pb2+ concentration. The feasibility of using cyanobacterium, P. angustissimum, as a living biosorbent for the extraction of Pb2+ from wastewater was studied by investigating its photosynthestic performance and tolerance under Pb2+ (0-5 mg L-1) contamination and low pH (pH 3-7). Decreased photosynthetic performance caused by Pb2+ contamination and low pH stress was detected in this study by means of a reduction of the maximum photochemical efficiency of PSII (F<inf>v</inf>/F<inf>m</inf>). Detoxification mechanisms of P. angustissimum on Pb2+ appeared to increase its intracellular polysaccharides (IPS), exocellular polysaccharides (EPS), and protein. Living P. angustissimum could increase the pH of the solution which resulted in Pb2+ precipitation. The unique ability of P. angustissimum to remove Pb2+ and to grow under toxic conditions, demonstrated herein, indicates that it is a promising living biosorbent for mildly acidic water contaminated with Pb2+ in bioremoval systems in the which pH is not lower than 5 and Pb2+ is not higher than 5 mg L-1. © 2013 Springer Science+Business Media Dordrecht.

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