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Author: search.filters.author.Rungjindamai, NattawutSubject: search.filters.subject.Actinomycetes

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    Item type:Publication,
    Nocardia xestospongiae sp. nov., isolated from a marine sponge in the Andaman sea
    (2017-05-01)
    Thawai, Chitti
    ;
    Rungjindamai, Nattawut
    ;
    Klanbut, Khanungkan
    ;
    Tanasupawat, Somboon
    A marine sponge-derived actinomycete, strain ST01-07<sup>T</sup>, was isolated from Xestospongia sp. collected from the Andaman Sea. The strain was characterised taxonomically using a polyphasic approach. The strain contained meso-diaminopimelic acid in the peptidoglycan, whole-cell sugars were arabinose, galactose, glucose, mannose and ribose. Mycolic acids that co-migrated with those from Nocardia araoensis NBRC 100135<sup>T</sup> were observed in whole-cell extracts. MK-8(H<inf>4ω-cycl</inf>) was the predominant menaquinone. Major cellular fatty acids were C<inf>17: 1</inf>ω8c, C<inf>16: 0</inf> and C<inf>17: 0</inf>. The diagnostic phospholipids in the cell consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain ST01-07<sup>T</sup> belonged to the genus Nocardia and was most closely related to N. araoensis IFM 0575<sup>T</sup> (98.71% 16S rRNA gene sequence similarity), Nocardia niwae W9241<sup>T</sup> (98.56%), Nocardia beijingensis AS4.1521<sup>T</sup> (98.41%) and Nocardia arthritidis IFM 10035<sup>T</sup> (98.36%). In addition, low DNA–DNA relatedness values (13.6±0.1% to 40.1±0.6%) confirmed that strain ST01-07<sup>T</sup> represents a novel species of the genus Nocardia, for which the name Nocardia xestospongiae sp. nov., is proposed. The type strain is ST01-07<sup>T</sup> (=BCC 45622<sup>T</sup>=NBRC 109069<sup>T</sup>).