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Author: search.filters.author.Saetiew, K.Subject: search.filters.subject.Mutation

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    Induced mutation of curcuma hybrid cv. sweetmemory through tissue culture by ethyl methanesulphonate (EMS)
    (2025-01-01)
    Yoosumran, V.
    ;
    Saetiew, K.
    ;
    Ruamrungsri, S.
    ;
    Akarapisarn, A.
    ;
    Teerarak, M.
    The ethyl methanesulphonate (EMS) is induced mutation in Curcuma hybrid cv. sweetmemory. The shoot size 1 cm were cultured on Murashige and Skoog medium (MS) supplemented with 2 mg/l BA for plantlet induction. After 8 weeks of culture, it was observed that the shoots soaked in 0% EMS at 60 and 120 minutes survived 100%. In additions it was found the highest percentage of shoot emergence, number of shoots, and plant height. While plants treated with EMS solution had decreased survival and growth rates. Thereafter, existing plantlets were potted, and growth was observed. It turned out that the plants none receiving EMS solution (control) had the survival rate and growth rate were better than those of the plants receiving EMS solution. Results showed that control plants receiving 0% EMS at 60 minutes gave highest content of chlorophyll a as 4.06 µg/cm<sup>2</sup>, chlorophyll b of 2.08 µg/cm<sup>2</sup> and carotenoid of 3.09 µg/cm<sup>2</sup> but there was none statistical difference with the plants that received EMS solution. The control plants had larger stomata sizes than those that received the EMS solution. However, there was no difference in the number of chloroplasts among treatments. The living plantlets from all treatments were tested Random amplified polymorphic DNA (RAPD) marker and genomic DNA was extracted from fresh leaves. A dendrogram was constructed polymorphic bands using the NTSYSpc program (version 2.10p) showing 2 clusters which separated with similarity coefficients ranging from 0.74-1.00.
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    Improvement of Dendranthemum grandiflora cv. canter with colchicine in vitro
    (2020-01-01)
    Asoko, N.
    ;
    Ruamrungsri, S.
    ;
    Yoosumran, V.
    ;
    Saetiew, K.
    The mutation of chrysanthemum with ray florets was induced by colchicine. The explants were cultured on Murashige and Skoog (MS) medium supplemented with 2 mg/l NAA and 4 mg/l Kinetin for inducing calli for 12 weeks. The shoots were regenerated from callus when cultured on MS medium for 8 weeks. The plant growth, size of stomata and number of chloroplasts per stomata were not statistically different in each treatment. However, the ray florets were received high levels of colchicine is less to survivor rate. The plantlet of chrysanthemum was transfered to pot plant containing soil and chopped coconut. Five of ten plant showed the flowers color changed from purple into a pinkish-orange at the 0.10% colchicine for 12 hours. The explant obtained from soaked of 0.15 % colchicine for 12 hours with the highest number of flowers per plant, 72 flowers per plant.