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    Item type:Publication,
    Impact of botanical essential oils on pupation and survival of Musca domestica L.
    (2026-05-01)
    Moungthipmalai, T.
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    Sriboonjit, N.
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    Khuendee, P.
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    Phunglakchai, J.
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    Thongsaiklaing, M.
    Among the tested botanical extracts, Illicium verum (star anise) essential oil at 10% demonstrated the highest efficacy in disrupting normal pupal development. Only 65.00% of individuals successfully emerged as fully developed adults after a 10-day exposure period. A notable proportion exhibited transitional deformities, particularly malformed pupal-adult intermediates, indicating interference with metamorphic progression. In comparison, cypermethrin, a commonly applied synthetic insecticide at the same concentration, resulted in a lower mortality rate of 39.67% under identical experimental conditions. The superior performance of star anise oil highlighted its potential as a plant-derived alternative for insect control. These findings suggested that the incorporation of such natural products into integrated pest management strategies may reduce dependence on conventional synthetic chemicals.
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    Item type:Publication,
    Larvicidal efficacy and morphological abnormalities induced by plant essential oils against housefly (Musca domestica L.)
    (2026-03-01)
    Passara, H.
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    Sittichok, S.
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    Moungthipmalai, T.
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    Laosinwattana, C.
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    Thongsaiklaing, M.
    The results showed that the lowest complete pupation rate was 43.67%, indicating a significant inhibition of normal metamorphosis. Additionally, 56.33% of third-instar larvae were classified as malformed larvae (ML), dying before pupation and exhibiting severe morphological and physiological abnormalities. These findings suggest that the star anise nanoemulsion interferes with key developmental pathways, preventing larvae from reaching the pupal stage. Due to its high effectiveness and plant-based origin, this nanoformulation offers a promising, environmentally friendly alternative to conventional chemical insecticides. It has potential for further development into a natural larvicidal agent for use in integrated pest management (IPM) strategies targeting housefly populations. Future research should explore the underlying mechanisms of action and assess the efficacy and safety of this nanoemulsion under real-world conditions to support its application in sustainable vector control.
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    Item type:Publication,
    Head lice prevalence in suburban Bangkok, Thailand, after the COVID-19 pandemic and herbal shampoo treatment
    (2026-01-01)
    Sinthusiri, J.
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    Sittichok, S.
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    Passara, H.
    ;
    Soonwera, M.
    The prevalence of pediculosis among 3,461 girls from 10 government kindergartens and primary schools in Ladkrabang district was 48.1% in the 1<sup>st</sup> semester (May-September 2022) and 44.6% in the 2<sup>nd</sup> semester (November 2022-March 2023), higher among primary school girls, at 51.8-55.4%, than in kindergarteners, at 30.2-33.1%. Over 80% of the infested girls came from a low-income family (<3,000 USD per year). The Zingiberaceae essential oil and shampoo treatments were highly effective with 100% cure rate for every infested girl after the 2<sup>nd</sup> treatment (on Day 10, after the 1<sup>st</sup> treatment on Day 1). Effective strategies for reducing head lice prevalence are improved living standards and head lice eradication by treatments with effective natural pediculicides and improved head lice educational program.
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    Item type:Publication,
    Plant essential oils, trans-anethole and eugenol, for housefly knockdown and mortality
    (2025-03-01)
    Sittichok, S.
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    Passara, H.
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    Sinthusiri, J.
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    Soonwera, M.
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    Thongsaiklaing, T.
    The knockdown and mortality of some compounds from natural oils (trans-anethole, eugenol and a combination of trans-anethole and eugenol) against adult houseflies (Musca domestica) compared with cypermethrin was investigated. The knockdown and mortalities were highest at 100% of 1% trans-anethole with KT50= 31 min and LT50 = 31 min. The mortality index of trans-anethole was 82.1 times that of cypermethrin. In contrast, 1% eugenol had knockdown and mortality was 36% and 0.5% trans-anethole + 0.5% eugenol had knockdown and mortality of 68%, with mortality indexes of 1.6 and 3.2 times versus cypermethrin. The trans-anethole high toxicity and low residue made it a superior control for houseflies.
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    Item type:Publication,
    Efficacy of plant essential oils for repelling against American cockroach adults (Periplaneta americana L.)
    (2025-01-01)
    Passara, H.
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    Soonwera, M.
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    Arhamad-Armeen, N.
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    Sittichok, S.
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    Jintanasirinurak, S.
    The combination of four plant essential oils (EOs) from star anise, citronella grass, lemon grass, and cinnamon was evaluated for repelling American cockroaches by the dual-choice method. A combination of star anise + citronella grass had a repellency rate of 95%. In contrast, combinations of star anise + lemon grass and star anise + cinnamon were effective between 73% and 69%, but the synthetic chemical repellent (12% DEET) was less effective at 59%. Therefore, the essential oils formulae had a high potential for effective use and, further, are harmless for healthiness and the environment. Thus, they can replace more toxic synthetic insecticides.
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    Item type:Publication,
    Larvicidal and pupicidal activity of combination of two plant essential oils against Aedes aegypti
    (2025-01-01)
    Sinthusiri, J.
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    Puwanard, C.
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    Passara, H.
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    Soonwera, M.
    The larvicidal and pupicidal effects of six mixtures of cinnamon (Cinnamomum verum J. Presl.) and nutmeg (Myristica fragrans Houtt.) essential oils (EOs) and major compositions (geranial, α-pinene and trans- cinnamaldehyde) were tested against early fourth instar and pupal stages of Aedes aegypti. The combinations (2% cinnamon EO + 1% geranial, 2% cinnamon EO + 1% trans-cinnamaldehyde and 2% cinnamon EO + 1% α-pinene) showed high toxicity against early 4<sup>th</sup> instar larvae, with 100% mortalities at 24 hours and LT50 between 1.4 and 2.4 hours. Additionally, 2% nutmeg EO + 1% α-pinene showed >99% mortality at 24 hours with LT50 = 4.6 hours. 2% nutmeg EO + 1% geranial and 2% nutmeg EO + 1% α-pinene were highly toxic against pupae, with 100% mortality at 48 hours and LT50 values of 5.8 and 7.8 hours. On the other hand, temephos showed LT50 for larval and pupal stages at 9.8 and 94.1 hours. It showed that 2% nutmeg EO + 1% α-pinene was able to control immature stages and was more effective than temephos. However nutmeg extracts need to be checked for human and valuable species toxicity.
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    Item type:Publication,
    Adulticidal activity of star anise, turmeric, cloves and combinations against houseflies
    (2025-01-01)
    Sinthusiri, J.
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    Soonwera, M.
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    Puwanard, C.
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    Passara, H.
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    Thongsaiklaing, T.
    The small droplet size could increase the absorbed ingredients and high stability. The star anise nanoemulsion was determined optimum efficacy, with a 1% concentration resulting in the highest knockdown and mortality rate of 100%. Its KT<inf>50</inf> and mortality effective values were 28 min and 36 times, respectively. Furthermore, the adult stage of houseflies is found to be susceptible to star anise nanoemulsion. In contrast, cypermethrin was less toxic to it, with a high KT<inf>50</inf> and resistance to adult houseflies. Consequently, the star anise nanoemulsion could be developed into an efficient and safe environment for controlling housefly populations.
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    Item type:Publication,
    Adulticidal effect of combinations between Cymbopogon citratus and Eucalyptus globulus essential oils against Pediculus humanus capitis De Geer
    (2023-07-01)
    Soonwera, M.
    ;
    Sittichok, S.
    The results showed that all head lice adults that were immersed in a combination of 15% C. citratus EO + 15% E. globulus EO, 20% C. citratus EO + 20% E. globulus EO, and 30% C. citratus EO + 30% E. globulus EO for 3 min were destroyed with the highest mortality rate of 100% at 30 min and an LT50 ranging from 1.2 to 1.5 min. However, 0.5% (w/w) permethrin shampoo provided a slightly higher adulticidal activity than all EO formulations in terms of LT50 (0.9 min). According to the LD50 values reported in the literature, C. citratus EO + E. globulus EO can be developed into a safer shampoo for head lice treatment.
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    Item type:Publication,
    Insecticidal activity of Citrus aurantium and Eucalyptus globulus essential oils and their major constituents against nymphs and adults of Pediculus humanus capitis De Geer
    (2023-07-01)
    Soonwera, M.
    ;
    Sittichok, S.
    The results showed that every tested EO and its constituents exhibited a higher pediculocidal activity against nymphs and adults of P. humanus capitis than 25% (w/v) benzyl benzoate did. In particular, 5% 1,8-cineole exhibited the highest insecticidal effects against P. humanus capitis nymphs and adults with 100% mortality rate at 1 min with an LT<inf>50</inf> of 0.2 min and an LC<inf>50</inf> of 0.7%. The other EO formulations provided a mortality rate ranging from 0-96.0% and an LT<inf>50</inf> ranging 0.3-315.9 min against nymphs. Against the adults, the mortality rate ranged from 12.0-96.0% with an LT<inf>50</inf> ranging from 1.2-314.5 min. Our data showed that 1,8-cineole has a great potential to control head lice populations and should be further developed into a safe and effective pediculicide.
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    Item type:Publication,
    Adulticidal activity against houseflies (Musca domestica L.; Muscidae: Diptera) of combinations of Cymbopogoncitratus and Eucalyptus globulus essential oils and their major constituents
    (2023-05-01)
    Moungthipmalai, T.
    ;
    Soonwera, M.
    The efficacies of Cymbopogon citratus, Eucalyptus globulus, eucalyptol, geranial, and their combined formulations against houseflies (Musca domestica) were evaluated and compared to cypermethrin, a common synthetic insecticide. Using a standard susceptibility assay that the World Health Organization (WHO) advises, the knockdown and mortality rates were calculated. The tested concentration range of these essential oils and their constituents was between 1% to 5%. The highest efficacy (100% knockdown rate and mortality rate) were provided by a combined formulation of 2% E. globulus EO + 1% geranial. This formulation had a KT<inf>50</inf> of 4.27 min. The two next best formulations were 2% C. citratus EO + 1% eucalyptol that provided a KT<inf>50</inf> of 5.85 min and an individual 5% C. citratus EO that provided a KT<inf>50</inf> of 6.69 min. Most importantly, several tested formulations provided a higher knockdown rate than 1% cypermethrin. Therefore, these formulations have a full potential as an alternative natural insecticide to replace cypermethrin.