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    Toxicity of Litsea petiolata Hook.f. essential oil against Aedes aegypti (Linn.), Aedes albopictus (Skuse), Anopheles minimus (Theobald) and Culex quinquefasciatus (Say)
    (2022-09-01)
    Puwanard, C.
    ;
    Moungthipmalai, T.
    ;
    Soonwera, M.
    This study evaluated the oviposition deterrent, ovicidal, larvicidal, pupicidal, and adulticidal activities of essential oil (EO) from Litsea petiolata leaves against Aedes aegypti, Aedes albopictus, Anopheles minimus and Culex quinquefasciatus with double choice, dipping, and contact assays. Litsea petiolata EO was tested at 1, 5, and 10% concentrations in ethanol and their efficiencies were compared with those of 1% w/w temephos and 10 % w/v cypermethrin. Oviposition deterrent was evaluated on gravid females. Larvicidal and pupicidal activities were tested on the fourth larvae and 2-day-old pupae. The adulticidal activity was tested against two-day-old adult females. Ten percent of L. petiolata EO exhibited the highest oviposition deterrent activity against gravid females and 100% repellency against Ae. albopictus and An. minimus, 97.0% against Ae. aegypti and 94.6% against Cx. quinquefasciatus. The oviposition activity index (OAI) against females of those four mosquito species ranged from -0.9 to -1.0. Ten percent of L. petiolata EO also exhibited the highest ovicidal activity against the eggs of the four mosquito species, with an inhibition rate ranging from 87.2 to 100%. Moreover, it also showed the highest larvicidal and puicidal activities against the larvae and pupae of the four mosquito species, with a 100% mortality rate at 10 and 60 min, respectively. The adulticidal activity was recorded at 1 and 24h. Ten percent of L. petiolata EO exhibited the highest toxicity to female adults of the four mosquito species, with 100% knockdown (1h) and mortality (24h) rates. On the other hand, 1%w/w temephos did not deter gravid females, and it was only slightly toxic to the eggs and larvae and non-toxic to the pupae. In the same manner, 10%w/v cypermethrin was less effective against the female adults of the four mosquito species than 10% L.petiolata EO. L.petiolata EO is a highly effective and eco-friendly alternative to synthetic insecticides.
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    Item type:Publication,
    Mosquito-repellent activity of Star anise (Illicium verum Hook.f.), Bustard cardamom (Amomum villosum Lour.) and Best cardamom (Amomum krervanh Pierre.) essential oils against Aedes albopictus (Skuse)
    (2020-01-01)
    Aungtikun, J.
    ;
    Soonwera, M.
    The mosquito repellent activities of essential oils derived from Illicium verum Hook.f., Amomum villosum Lour. and Amomum krervanh Pierre. against Aedes albopictus females were compared with that of a chemical repellent (DEET; N, N-diethyl-3-methylbenzamide). The results showed that the three essential oils at the concentrations of 10% and 5% provided the same 15-minute protection from Ae. albopictus. The essential oils at 1% also provided some 3-minute protection. Comparing the essential oils, it was found that the essential oils at 5% concentration provided the most effective and safe protection against Ae. albopictus, with a protection time of 15minutes, the higher concentration, 10%, provided the same protection rate. DEET at concentrations of 1%, 5% and 10% provided protection times of 15, 30 and 45 minutes, respectively. It can be concluded that the essential oils extracted from I. verum, A. villosum, A.krervanh have a full potential as effective protection against Ae. albopictus females. Although the essential oils were less effective than the synthetic chemical repellent, they are safer to use. It is hereby recommeded that these essential oils were good alternatives and should be further developed into commercial repellent products.
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    Item type:Publication,
    Efficacies of essential oils from illiciaceae and zingiberaceae plants as oviposition deterrent, ovicidal, and adulticidal agents against females aedes albopictus (Skuse) and anopheles minimus (Theobald)
    (2018-09-01)
    Cotchakaew, N.
    ;
    Soonwera, M.
    At present, serious mosquito vectors throughout the world have developed resistance to chemical insecticides.Therefore, safe natural products for controlling mosquitoes are absolutely necessary. This study investigated the efficacy of herbal essential oil (EO) from Illiciaceae and Zingiberaceae plants, Alpinia galanga (A. galanga), Amomum krervanh (A. krervanh), Curcuma zedoaria (C. zedoaria), Illicium verum (I. verum), Zingiber cassumunar (Z. cassumunar) and Zingiber mekongense (Z. mekongense) against the females of Aedes albopictus (Ae. albopictus) and Anopheles minimus (An. minimus). All EOs at the high concentration (10%) were highly effective in oviposition deterrent, ovicidal, and adulticidal activities. Ten percent of C. zedoaria EO showed 100% effective repellency with an oviposition activity index (OAI) of -1.0 against the females of the two mosquito species, and 10% I.verum EO showed a high inhibition rate of 100% against the eggs of the two mosquitoes. Ten percent of A. galanga EO showed a high adulticidal activity against the females of Ae. albopictus and An. minimus with KT50 of 0.7 and 1 min at 1h, respectively, and 100% mortality at 24h as well as an LC50 of 7.5 and 2.9%, respectively. When compared with temephos and cypermethrin, the EOs from C. zedoaria, I.verum and A. galanga were more effective in oviposition deterrent and ovicidal activities than temephos and were equivalent in adulticidal activity to cypermethrin. To conclude, our data show that EOs from C. zedoaria, I.verum and A. galanga can be used as an oviposition deterrent, ovicidal and adulticidal agents against Aedes albopictus and Anopheles minimus.