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AI Chatbot for Post-Operative Oral Surgery Information and Support
(2025-08-07)
Warin, Kritsasith
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Taetragoo, Unchalisa
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Trachoo, Vorapat
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Khanijoh, Chanon
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Saepong, Pechdanai
This study presented the development of an AI chatbot specifically designed to address oral surgery-related inquiries. The chatbot, trained on a dataset of 240 questions, utilized machine learning algorithms to predict the corresponding operation for each question. The best model achieved accuracy of 0.906 in responding to questions. In conclusion, this AI chatbot has the potential to improve patient care by providing clear information to enhance post-operative outcomes.
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Automated Classification of User Exercise Poses in Virtual Reality Using Machine Learning-Based Human Pose Estimation
(2025-01-01)
Kongchansawang, Panuwat
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Naowavathong, Thunchanok
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Srisuttee, Ratakorn
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Moodleah, Samart
Human Pose Estimation (HPE) using machine learning presents significant potential for objectively analyzing user movements within Virtual Reality (VR) environments, particularly for applications involving guided physical exercises or interactive tasks. Analyzing 2D video footage from VR sessions for HPE offers a computationally efficient approach for tracking user movements. However, accuracy can be compromised by various factors, including occlusions from clothing or the VR headset itself. This study develops and evaluates a robust HPE system specifically designed to accurately classify predefined poses performed by users within such VR environments. The proposed system utilizes MediaPipe for corporal landmark extraction from user images (for training) and employs a Stacking Classifier ensemble, with XGBClassifier as the meta-learner, to classify six key exercise poses. Key results demonstrate an overall pose classification accuracy of 94% on a dedicated test set, with certain poses like ‘Butterfly Hug’ and ‘Arm’ achieving 100% accuracy. These findings highlight the system’s potential to provide reliable, quantitative assessments of user adherence to prescribed movements in immersive interactive environments, offering valuable data for applications requiring objective analysis of physical performance in VR.
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Determining the Optimal Parameters for Preventing Backward Falls Using a Human Movement Dataset: An Investigative Study
(2024-01-01)
Yoneyama, Keito R.
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Sermswan, Anawat
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Jansri, Ukkrit
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Srisuttee, Ratakorn
Falls are a major health concern, particularly for the elderly, as they often lead to severe injuries like hip fractures. Fortunately, there are a lot of publicly available datasets that can offer valuable insights regarding the mechanics of falls and activities of daily living. This study aims to determine the optimal prevention threshold for backward falls using available datasets, thereby avoiding any fall-related injuries. The methodology employed in this study involves identifying downward trends of acceleration and angular velocity on the graph. The Pythagorean Theorem is used to calculate the resultant acceleration, while differences are computed to determine the changes in angular velocity. The results from 150 samples shows that the acceleration for the prevention threshold range is between 3.016m/s2 - 4.308 m/s2and the average angular velocity is 0.522 rad/s - 0.746 rad/s which not only prevents a person from falling backward but will also be able to distinguish between activities of daily living. In conclusion, using existing dataset can offer essential knowledge into fall detection and serves as a foundation for determining the optimal fall prevention threshold.
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Assessment of in vitro anti-skin aging activities of Phyllanthus indofischeri Bennet extracts for dermatological and aesthetic applications
(2023-12-01)
Boonpisuttinant, Korawinwich
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Taka, Thanachai
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Ruksiriwanich, Warintorn
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Chutoprapat, Romchat
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Udompong, Sarinporn
Giant Indian Gooseberry (GIG) or Phyllanthus indofischeri Bennet are commercially cultivated and commonly used herbs in Traditional medicine, especially in Thailand. The aim of this study was to assess the potential of the GIG extracts in anti-aging activities to be a dermatological application. The juice, meat residues, and seeds of GIG collected from Sra Kaeo Province, Thailand, were extracted by the Boiling method (B) and the Maceration process (M) by using 95% ethanol as a solvent. The GIG extracts gave the total phenolic, total flavonoid contents and quercetin contents, as well as exhibited anti-oxidative activities. The GIG-R-B extract inhibited tyrosinase activity and had the highest anti-melanogenesis activity on B<inf>16</inf>F<inf>10</inf> cells at 31.63 ± 0.70%. The GIG-S-B, GIG-S-M, and GIG-R-M extracts demonstrated the highest collagen biosynthesis, which was comparable to vitamin C (p < 0.05), whereas the GIG-R-B extracts gave the highest stimulation of anti-aging genes (SIRT1 and FOXO1). All extracts at the concentration of 0.1 mg/mL showed no cytotoxicity on human skin fibroblasts. Therefore, the GIG-S-B extract was discovered to be a promising natural anti-aging agent for dermatological health and aesthetic applications that can be further developed in cosmetic, functional food and food supplement industries.
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In vitro anti-ageing activities of ethanolic extracts from Pink rambutan (Nephelium lappaceum Linn.) for skin applications
(2023-04-01)
Boonpisuttinant, Korawinwich
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Srisuttee, Ratakorn
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Yen Khong, Heng
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Chutoprapat, Romchat
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Ruksiriwanich, Warintorn
Skin ageing is characterized by features such as wrinkles, loss of elasticity, laxity, rough-textured appearance, melasma and freckles. Several researches have focused for preventing, and treating skin ageing by many natural ingredients. This study aimed to assess the anti-ageing activities for anti-skin ageing of the ethanolic extracts of Pink rambutan (PR) (Nephelium lappaceum Linn.) from leaves (L), branches (B), seeds (S), and peels from ripe (R) and young (Y) fruits. The extraction yields of all Pink Rambutan (PR) extracted by the Maceration (M) and the Soxhlet extraction (Sox) using 95% ethanol as a solvent, ranged from 10.62% to 30.63%. Flavonoids were found as the main phytochemicals in almost all the PR extracts. The PR-Y-M and PR-Y-Sox extracts gave the highest total phenolic contents by the Folin-Ciocalteu assay of 67.60 ± 4.38 mgGAE/g, and total flavonoid contents by the modified aluminum chloride colorimetric assay of 678.72 ± 23.59 mgQE/g, respectively. The PR-L-M extracts showed the highest three anti-oxidative activities; the free radical scavenging (SC<inf>50</inf> of 0.320 ± 0.070 mg/mL), the lipid peroxidation inhibition (LC<inf>50</inf> of 0.274 ± 0.029 mg/mL), and the metal chelation activity (MC<inf>50</inf> of 0.203 ± 0.021 mg/mL). All the PR extracts at 0.01 and 0.1 mg/mL showed no cytotoxicity on B<inf>16</inf>F<inf>10</inf> cells, and human skin fibroblasts, respectively. Likewise, the PR-R-Sox extract exhibited the highest anti-melanogenesis on B<inf>16</inf>F<inf>10</inf> cells (52.7 ± 0.9%) and, the mushroom tyrosinase inhibition activity (IC<inf>50</inf> of 0.04 ± 0.02 mg/mL), which was significantly comparable to kojic acid (p < 0.05). The PR-Y-Sox extract showed the collagen biosynthesis by the Sirius Red method, and the stimulation of anti-ageing genes (Sirt1 and Foxo1) on human skin fibroblasts by the RT-PCR method, which were similar to standards ʟ-ascorbic acid and resveratrol, respectively. This study suggests that the PR-R-Sox and PR-Y-Sox extracts can be further developed as natural anti-ageing agents for whitening and anti-wrinkle in the cosmetics, cosmeceutical, and pharmaceutical industries.
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Induction of ZCCHC6 expression in peripheral blood mononuclear cells by HNSCC secretions
(2020-09-05)
Arayataweegool, Areeya
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Srisuttee, Ratakorn
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Bin-Alee, Fardeela
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Mahattanasakul, Patnarin
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Tangjaturonrasme, Napadon
Cancer secretion can change the properties of adjacent cells, including peripheral blood mononuclear cells (PBMCs). We investigated whether such secretion influences messenger RNA expression in PBMCs of patients with head and neck squamous cell carcinoma (HNSCC). In the present study, co-culture model of normal PBMCs and HNSCC cell lines were established. The PBMCs were subsequently subjected to RNA sequencing for transcriptome analysis. Furthermore, expression data from the Gene Expression Omnibus repository, platform GPL4133, series GSE39400, were gathered to analyze, afterward identify zinc finger CysCysHisCys (CCHC)–type domain-containing protein 6 (ZCCHC6) as the main gene involved in HNSCC. This gene was then validated by a quantitative real-time polymerase chain reaction. The results showed that ZCCHC6 was expressed at significantly higher levels in the patients with HNSCC than in the healthy controls, and the sensitivity and specificity of these findings for diagnostic purposes were 100.00% and 70.83%, respectively. In summary, our findings demonstrated that the secretion of HNSCC cells could cause the alterations in messenger RNA expression by PBMCs. The ZCCHC6 expression level may apply in HNSCC screening.
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Evaluation of NID2 promoter methylation for screening of Oral squamous cell carcinoma
(2020-03-14)
Srisuttee, Ratakorn
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Arayataweegool, Areeya
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Mahattanasakul, Patnarin
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Tangjaturonrasme, Napadon
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Kerekhanjanarong, Virachai
Background: Oral squamous cell carcinoma (OSCC) is an aggressive human malignancy. Because of late diagnosis and recurrence of OSCC, the treatment of patients with OSCC is often ineffective. Thus, finding novel biomarkers of OSCC are essential. Here we derived a methylation marker by utilizing methylation microarray data and testing its capacity in cross-sectional study designed for OSCC detection and screening. Methods: According to bioinformatics analysis of total of 27,578 cg sites, cg22881914 of Nidogen 2 (NID2) methylation was selected for evaluation. Next, we confirmed the methylation status by bisulfite sequencing from the microdissected OSCC cells in comparison with the microdissected oral epithelia. Subsequently, we developed a simple technique using real-time PCR with the specific probe to examine the ability for the detection of OSCC in the oral epithelial samples, which included 103 oral rinse and 82 oral swab samples. Results: Based on the comparison of microdissected tissue, cg22881914 of NID2 was proved to be methylated in most OSCC cells but unmethylated in the normal oral epithelia. Furthermore, the methylated NID2-relied quantitative PCR approach has demonstrated that this marker assists in distinguishing among patients with OSCC from normal oral epithelia, smokers, and patients with oral lichen planus using the non-invasive oral rinse and swab samples. Conclusions: Specific methylation at cg22881914 of NID2 of OSCC could be used as an important potential marker for detecting OSCC. Thus, to certify the utility of this marker, further studies with a larger sample size are needed.
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Cellular expression profiles of Epstein-Barr virus-transformed B-lymphoblastoid cell lines
(2020-01-01)
Chaiwongkot, Arkom
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Kitkumthorn, Nakarin
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Srisuttee, Ratakorn
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Buranapraditkun, Supranee
Epstein-Barr virus (EBV) can infect human B cells and is associated with various types of B cell lymphomas. Studies on the global alterations of the cellular pathways mediated by EBV-induced B cell transformation are limited. In the present study, microarray analysis was performed following generation of two EBV-infected B-lymphoblastoid cell lines (BLCL), in which normal B cells obtained from two healthy Thai individuals and transcriptomic profiles were compared with their respective normal B cells. The two EBV-transformed BLCL datasets exhibited a high degree of similarity between their RNA expression profiles, whereas the two normal B-cell datasets did not exhibit the same degree of similarity in their RNA expression profiles. Differential gene expression analysis was performed, and the results showed that EBV infection was able to dysregulate several cellular pathways in the human B-cell genes involved in cancer and cell activation, such as the MAPK, WNT and PI3K-Akt signaling pathways, which were upregulated in the BLCL and were associated with increased cellular proliferation and immortalization of EBV-infected B cells. Expression of proteins located in the plasma membrane, which initiate a biological response to ligand binding, were also notably upregulated. Expression of genes involved in cell cycle control, the p53 signaling pathway and cellular senescence were downregulated. In conclusion, genes that were markedly upregulated by EBV included those involved in the acquisition of a tumorigenic phenotype of BLCL, which was positively correlated with several hallmarks of cancer.
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Head and neck squamous cell carcinoma drives long interspersed element-1 hypomethylation in the peripheral blood mononuclear cells
(2019-01-01)
Arayataweegool, Areeya
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Srisuttee, Ratakorn
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Mahattanasakul, Patnarin
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Tangjaturonsasme, Napadon
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Kerekhanjanarong, Virachai
Objective: Alteration of long interspersed element-1 (LINE-1) methylation in peripheral blood mononuclear cells (PBMCs) has been simultaneously activated to breast carcinogenesis due to its secretion. To evaluate the effect in head and neck squamous cell carcinoma (HNSCC), LINE-1 methylation levels and patterns have been measured both in vitro and in vivo. Methods: Analysis of LINE-1 methylation in cocultured models between HNSCC cell lines and normal PBMCs was performed. The observation of PBMCs of HNSCC patients compared to PBMCs of normal controls was performed using the semiquantitative combined bisulfite restriction analysis technique. Results: Downregulation of LINE-1 methylation was significantly found in the PBMCs cocultured with all HNSCC cell lines compared to normal PBMCs. Likewise, a reduction in LINE-1 methylation levels was observed in PBMCs of HNSCC compared to normal PBMCs (p < 0.0001). Receiver operating characteristic analysis demonstrated the potential of the unmethylated alleles (uCuC) of LINE-1 for distinguishing the PBMCs of HNSCC patients from normal controls with 100% sensitivity and specificity. Conclusion: Our data supported that the alteration of LINE-1 methylation levels in PBMCs was influenced by HNSCC secretions. Moreover, the unmethylated LINE-1 allele of PBMCs was proved to be an effective tumor marker and possesses a potential as HNSCC diagnostic tool.

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