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Author: search.filters.author.Supong, KhomsanStart date: 2020

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    Streptomyces odontotermitis Odt1-20BT, an actinobacterium isolated from the fungus-growing Odontotermes formosanus
    (2026-08-01)
    Supong, Khomsan
    ;
    Niemhom, Nantawan
    ;
    Suriyachadkun, Chanwit
    ;
    Tanasupawat, Somboon
    ;
    Pittayakhajonwut, Pattama
    A novel culturable insect-derived actinobacterium, designated strain Odt1-20B<sup>T</sup>, was isolated from Odontotermes formosanus. The cells exhibited aerobic, Gram-stain-positive, and filamentous characteristics. Strain Odt1-20B<sup>T</sup> formed well-developed substrate and aerial mycelia, with straight spore chains borne on the aerial mycelium. Strain Odt1-20B<sup>T</sup> grew at 20–40 °C (optimum 30 °C), 0˗4% (w/v) NaCl, and pH 5˗10 (optimum 7−8). The l,l-diaminopimelic acid was determined in the whole-cell hydrolysate of strain Odt1-20B<sup>T</sup>. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine, whereas the detected major quinones were MK-9(H<inf>4</inf>), MK-9(H<inf>6</inf>), and MK-9(H<inf>8</inf>). Whole-cell sugars were galactose, glucose, mannose, ribose, and xylose. Summed feature C<inf>18:1</inf> ω6c/ω7c, C<inf>16:0</inf>, anteiso-C<inf>15:0</inf>, and iso-C<inf>16:0</inf> were the main fatty acids. The draft genome size was 10,091,787 nucleotides, with a G + C content of 71.5%. Comparative phylogenetic analysis based on 16S rRNA gene sequence demonstrated that strain Odt1-20B<sup>T</sup> belonged to the genus Streptomyces, and shared the highest similarity with S. davaonensis JCM 4913 <sup>T</sup> (98.55%). Furthermore, phylogenomic analysis showed that strain Odt1-20B<sup>T</sup> was clearly distinguished from the closely related Streptomyces species. The average nucleotide identity analyses based on BLAST (ANIb) and MUMmer (ANIm) between strain Odt1-20B<sup>T</sup> and its related species ranged below 87%, and the digital DNA-DNA hybridization (dDDH) values ranged from 21.2% to 29.9%, all of which are below the species delimitation thresholds. The results of polyphasic taxonomy clearly indicated that strain Odt1-20B<sup>T</sup> represents a novel species, proposed as Streptomyces odontotermitis sp. nov. with the type strain Odt1-20B<sup>T</sup> (= NBRC 116112 <sup>T</sup> = TBRC 16156 <sup>T</sup>).
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    cis-Decalinoyltetramic Acid Derivatives from the Palm Fungus Leptosporella arengae TBRC-BCC32590
    (2025-11-28)
    Intaraudom, Chakapong
    ;
    Bunbamrung, Nantiya
    ;
    Dramae, Aibrohim
    ;
    Nithithanasilp, Sutichai
    ;
    Boonyuen, Nattawut
    Twelve hitherto undescribed cis-decalinoyltetramic acid derivatives (arengasetins A – L), a unique spiro[2.4]heptendione (spiroarengarone) and two benzoquinones (arengaquinones A and B), together with the highly oxygenated polycyclic molecule, epicolactone, were isolated from the palm fungus Leptosporella arengae TBRC-BCC 32590. Chemical structures were determined by extensive analyses of 2D NMR spectroscopic data and chemical means. The absolute configuration of arengasetin A (1) was confirmed by X-ray crystallographic information, while the absolute configurations of other related compounds were corroborated by comparison of NMR spectroscopic data and chemical reactions. The isolated compounds exhibited activity against Plasmodium falciparum (K1, multidrug-resistant strain, IC<inf>50</inf> 6.42 – > 38.76 μM), Bacillus cereus (MIC 0.78 – > 50.0 μg/mL), Staphylococcus aureus (MIC 1.56 – > 50.0 μg/mL), and Mycobacterium tuberculosis (MIC 6.25 – > 50.0 μg/mL). Cytotoxicity against both cancerous (MCF-7 and NCI-H187) and noncancerous (Vero) was also reported.
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    Actinomycetospora termitidis sp. nov., an insect-derived actinomycete isolated from termite (Odontotermes formosanus)
    (2024-05-01)
    Supong, Khomsan
    ;
    Niemhom, Nantawan
    ;
    Suriyachadkun, Chanwit
    ;
    Phongsopitanun, Wongsakorn
    ;
    Tanasupawat, Somboon
    Strain Odt1-22<sup>T</sup>, an insect-derived actinomycete was isolated from a termite (Odontotermes formosanus) that was collected from Chanthaburi province, Thailand. Strain Odt1-22<sup>T</sup> was aerobic, Gram-stain-positive, and produced bud-like spore chain on the substrate hypha. According to chemotaxonomic analysis, strain Odt1-22<sup>T</sup> contained meso-diaminopimelic acid in peptidoglycan and the whole-cell hydrolysates contained arabinose, galactose, glucose, and ribose. The major menaquinone was MK-8(H<inf>4</inf>). The diagnostic phospholipids were diphosphatidylglycerol, hydroxyphosphatidylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis based on 16 S rRNA gene sequence revealed that strain Odt1-22<sup>T</sup> was identified to the genus Actinomycetospora and showed high similarity values with A. chiangmaiensis DSM 45062 <sup>T</sup> (99.24%), A. soli SF1<sup>T</sup> (99.24%) and A. corticicola 014-5 <sup>T</sup> (98.17%). The genomic size of strain Odt1-22<sup>T</sup> was 6.6 Mbp with 73.8% G + C content and 6355 coding sequences (CDSs). The genomic analysis, strain Odt1-22<sup>T</sup> and closely related species A. chiangmaiensis DSM 45062 <sup>T</sup>, A. soli SF1<sup>T</sup> and A. corticicola DSM 45772 <sup>T</sup> displayed the values of average nucleotide identity-blast (ANIb) at 83.7–84.1% and MUMmer (ANIm) at 86.6–87.0%. Moreover, the results of digital DNA-DNA hybridization values between strain Odt1-22<sup>T</sup> and related Actinomycetospora species were 45.8−50.5% that lower than the threshold value of commonly used to delineate separated species level. On the basis of phenotypic, chemotaxonomic, and genotypic data, strain Odt1-22<sup>T</sup> represented a novel species within the genus Actinomycetospora, for which the name Actinomycetospora termitidis sp. nov. is proposed. The type strain of the species is Odt1-22<sup>T</sup> (= TBRC 16192 <sup>T</sup> = NBRC 115965 <sup>T</sup>).
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