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Author: search.filters.author.Suriyachadkun, ChanwitSubject: search.filters.subject.Actinomycetes

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    Streptomyces telluris sp. nov., a promising terrestrial actinobacterium with antioxidative potentials
    (2023-06-01)
    Thayanuwadtanawong, Onnicha
    ;
    Duangupama, Thitikorn
    ;
    Bunbamrung, Nantiya
    ;
    Pittayakhajonwut, Pattama
    ;
    Intaraudom, Chakapong
    An actinomycete strain, AA8<sup>T</sup>, which produced a long straight chain of spores (verticillati type), was isolated from the rhizosphere soil of Mangifera indica in Bangkok, Thailand. A polyphasic taxonomic study was carried out to establish the taxonomic position of the strain. Strain AA8<sup>T</sup> formed a tight taxonomic position in the 16S rRNA gene tree with Streptomyces roseifaciens MBT76<sup>T</sup>. In contrast, the genome-based taxonomic analysis showed that strain AA8<sup>T</sup> shared low average nucleotide identity-BLAST (94.1%), the digital DNA–DNA hybridization (58.2%), and the average amino acid identity (93.6%) values with S. roseifaciens MBT76<sup>T</sup>. Moreover, a combination of physiological and biochemical properties indicated that strain AA8<sup>T</sup> was distinguished from all Streptomyces species with effectively published names. Strain AA8<sup>T</sup>, therefore, represents a novel species of Streptomyces, and the name Streptomyces telluris is proposed for the strain. The type strain is AA8<sup>T</sup> (= TBRC 8483<sup>T</sup> = NBRC 113461<sup>T</sup>). The chemical investigation led to the isolation of nine known compounds (compounds 1–9). Among these compounds, compound 7 (3,4-dihydroxybenzaldehyde) possesses strong antioxidant activity equal to ascorbic acid, a powerful antioxidative agent.
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    Streptomyces musisoli sp. nov., an actinomycete isolated from soil
    (2021-01-01)
    Duangupama, Thitikorn
    ;
    Intaraudom, Chakapong
    ;
    Pittayakhajonwut, Pattama
    ;
    Suriyachadkun, Chanwit
    ;
    Tadtong, Sarin
    An actinobacterium, strain CH5-8<sup>T</sup>, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp. The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium. The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8<sup>T</sup>, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H<inf>8</inf>), MK-9(H<inf>6</inf>), and MK-9(H<inf>4</inf>) as major menaquinones and anteiso-C<inf>15:0</inf>, iso-C<inf>16:0</inf>, and anteiso-C<inf>17:0</inf> as major fatty acids. Diphosphatidylglycerol, phosphatidylethanola-mine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells. A combination of morphological and chemotaxonomic data supported the assignment to the genus Strep-tomyces. The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain. The novel strain CH5-8<sup>T</sup> showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763<sup>T</sup> (98.9%), followed by Streptomyces actinomycinicus RCU-197<sup>T</sup> (98.9%). The average nucleotide identity by blast (ANIb) and digital DNA–DNA hybridization values between CH5-8<sup>T</sup> and its closest relatives, S. echinatus CECT 3313<sup>T</sup> and S. actinomycinicus RCU-197<sup>T</sup>, were ≤91.6% and ≤47.4%, respectively. The digital DNA G+C content of genomic DNA was 72.1 mol%. On the basis of these phenotypic and genotypic data, strain CH5-8<sup>T</sup> represents a novel species, for which the name Streptomyces musisoli sp. nov. is proposed. The type strain is CH5-8<sup>T</sup> (=TBRC 9950<sup>T</sup>=NBRC 113997<sup>T</sup>).
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    Micromonospora spongicola sp. nov., an actinomycete isolated from a marine sponge in the Gulf of Thailand
    (2013-09-01)
    Supong, Khomsan
    ;
    Suriyachadkun, Chanwit
    ;
    Pittayakhajonwut, Pattama
    ;
    Suwanborirux, Khanit
    ;
    Thawai, Chitti
    An actinomycete strain, S3-1 T, was isolated from marine sponge sample collected from the Gulf of Thailand. The strain is aerobic, Gram-positive and produced single spores at the tip of the substrate mycelium. Strain S3-1 T contained meso-diaminopimelic acid in the peptidoglycan, whole-cell sugars were arabinose, galactose, glucose, rhamnose, ribose and xylose. The polar lipid profile of strain S3-1 T consisted of phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, phosphoglycolipid and unknown polar lipids. Morphological and chemotaxonomic characteristics of the strain were identified as a member of the genus Micromonospora. Phylogenetic analysis based on 16S rRNA gene sequence analysis of the strain showed similarity to Micromonospora nigra DSM 43818 T (98.8%), Micromonospora yangpuensis FXJ6.011 T (98.7%) and Micromonospora narathiwatensis BTG4-1 T (98.6%). The DNA G+C content was 72.7 mol%. The phenotypic characteristics and DNA-DNA relatedness values supported that the classification of this strain as a novel species in the genus Micromonospora, for which the name Micromonospora spongicola sp. nov. (type strain S3-1 T =BCC 45595 T =NBRC 108779 T) is proposed. © 2013 Japan Antibiotics Research Association All rights reserved.