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Author: search.filters.author.Sutjaritvorakul, ThanawatSubject: search.filters.subject.Plant regeneration

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    Application of activated charcoal and nanocarbon to callus induction and plant regeneration in aromatic rice (Oryza sativa L.)
    (2018-01-01)
    Chutipaijit, Sutee
    ;
    Sutjaritvorakul, Thanawat
    The investigations of nanotechnology with the application on agricultural products also have been few reported, especially the plant regeneration. The effects of activated charcoal and nanocarbon on the callus induction and plant regeneration of aromatic rice were studied. Activated charcoal was added into the callus induction and regeneration medium. The presence of activated charcoal in the callus induction medium (100-500 mg L<sup>−1</sup>), activated charcoal significantly reduced the percentage of the callus induction and biomass accumulation (fresh weight, dry weight and size). Whereas, the regeneration medium supplemented with 100 mg L<sup>−1</sup> of activated charcoal showed the highest percentage of plant regeneration (61.90%) and the ratio of the number of seedlings to the number of regenerated calli (RSR; 3.06) that derived from the callus induction medium (without activated charcoal). Moreover, the induced calli derived from the callus induction medium supplemented with nanocarbon at 5 mg L<sup>−1</sup> showed the highest percentage of callus induction (94.70%), the percentage of green spots (95.83%), the percentage of plant regeneration (60.42%) and the RSR (3.12) when transferred the calli into the regeneration medium (without nanocarbon). After that, nanocarbon was also added into the regeneration medium. The percentage of green spots (96.08%), the percentage of plant regeneration (62.75%) and the RSR (3.16) obtained from the regeneration medium supplemented with 20 mg L<sup>−1</sup> of nanocarbon showed the highest values. This experiment showed that the optimum concentration of activated charcoal and nanocarbon had potential to enhance the callus induction and plant regeneration frequencies in tissue culture medium of aromatic rice.
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    Application of nanomaterials in plant regeneration of rice (Oryza sativa L.)
    (2017-01-01)
    Chutipaijit, Sutee
    ;
    Sutjaritvorakul, Thanawat
    In recent years, the application of nanomaterials has been investigated by various studies, especially in their biological and agricultural features. This research aimed to evaluate the influence of zinc oxide (ZnO) and titanium dioxide (TiO<inf>2</inf>) nanoparticles on the quality of the plant regeneration in rice (Oryza sativa L. cv. RD49). Mature seed explants were used for callus induction and plant regeneration cultured on the medium supplemented with different concentrations of ZnO or TiO<inf>2</inf> nanoparticles. The high percentage of the callus induction was achieved on the medium supplemented with 5 mg.L<sup>-1</sup> ZnO nanoparticles or 20 mg.L<sup>-1</sup> TiO<inf>2</inf> nanoparticles when compared with control treatment (without nanoparticles). Various concentrations of TiO<inf>2</inf> nanoparticles were also found to increase the percentage of the plant regeneration more than ZnO nanoparticles. The highest percentage of plant regeneration was observed on the medium containing the concentration of 20 mg.L<sup>-1</sup> TiO<inf>2</inf> nanoparticles. The in vitro rooting of regenerated shoots was hardened and successfully transplanted to the field after 30 days of transplantation. The results suggested that ZnO and TiO<inf>2</inf> nanomaterials may have potential applications in the in vitro plant regeneration.