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Author: search.filters.author.Tadtong, SarinSubject: search.filters.subject.16S rRNA gene

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    Streptomyces telluris sp. nov., a promising terrestrial actinobacterium with antioxidative potentials
    (2023-06-01)
    Thayanuwadtanawong, Onnicha
    ;
    Duangupama, Thitikorn
    ;
    Bunbamrung, Nantiya
    ;
    Pittayakhajonwut, Pattama
    ;
    Intaraudom, Chakapong
    An actinomycete strain, AA8<sup>T</sup>, which produced a long straight chain of spores (verticillati type), was isolated from the rhizosphere soil of Mangifera indica in Bangkok, Thailand. A polyphasic taxonomic study was carried out to establish the taxonomic position of the strain. Strain AA8<sup>T</sup> formed a tight taxonomic position in the 16S rRNA gene tree with Streptomyces roseifaciens MBT76<sup>T</sup>. In contrast, the genome-based taxonomic analysis showed that strain AA8<sup>T</sup> shared low average nucleotide identity-BLAST (94.1%), the digital DNA–DNA hybridization (58.2%), and the average amino acid identity (93.6%) values with S. roseifaciens MBT76<sup>T</sup>. Moreover, a combination of physiological and biochemical properties indicated that strain AA8<sup>T</sup> was distinguished from all Streptomyces species with effectively published names. Strain AA8<sup>T</sup>, therefore, represents a novel species of Streptomyces, and the name Streptomyces telluris is proposed for the strain. The type strain is AA8<sup>T</sup> (= TBRC 8483<sup>T</sup> = NBRC 113461<sup>T</sup>). The chemical investigation led to the isolation of nine known compounds (compounds 1–9). Among these compounds, compound 7 (3,4-dihydroxybenzaldehyde) possesses strong antioxidant activity equal to ascorbic acid, a powerful antioxidative agent.
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    Streptomyces epipremni sp. nov., an endophytic actinomycete isolated from the root of Epipremnum aureum
    (2022-01-01)
    Duangupama, Thitikorn
    ;
    Intaraudom, Chakapong
    ;
    Pittayakhajonwut, Pattama
    ;
    Tadtong, Sarin
    ;
    Thawai, Chitti
    An endophytic Streptomyces-like micro-organism, designated strain PRB2-1<sup>T</sup> was isolated from root tissue of Epipremnum aureum (Linden and André) G.S. Bunting. The typical morphological and chemotaxonomic characteristics, i.e. the ability to produce straight spore chains directly on aerial mycelium and the presence of ll-diaminopimelic acid in cell-wall peptidogly-can, were consistent with its assignment to the genus Streptomyces. 16S rRNA gene analysis showed that strain PRB2-1<sup>T</sup> is a member of the genus Streptomyces with the highest similarity to Streptomyces bryophytorum DSM 42183<sup>T</sup> (98.4 %). Moreover, the draft genome sequence of strain PRB2-1<sup>T</sup> exhibited low average nucleotide identity by blast (79.9–83.8 %) and digital DNA– DNA hybridization (24.9–28.3 %) values to the reference strains, which were well below the species circumscription threshold. The DNA G+C content of genomic DNA was 73.6 mol%. Comparison of phenotypic characteristics and whole-genome sequence between strain PRB2-1<sup>T</sup> and its close relatives indicated that strain PRB2-1<sup>T</sup> could be classified as a novel species of the genus Streptomyces. Thus the name, Streptomyces epipremni sp. nov. is proposed for the strain. The type strain is PRB2-1<sup>T</sup> (=TBRC 7642<sup>T</sup>=NBRC 113169<sup>T</sup>).
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    Streptomyces endocoffeicus sp. nov., an endophytic actinomycete isolated from Coffea arabica (L.)
    (2021-11-01)
    Nammali, Achararak
    ;
    Intaraudom, Chakapong
    ;
    Pittayakhajonwut, Pattama
    ;
    Suriyachadkun, Chanwit
    ;
    Tadtong, Sarin
    An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110<sup>T</sup>, was isolated from the surface-sterilised root of Coffea arabica L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110<sup>T</sup> was a member of the genus Streptomyces and showed the closest similarities to Streptomyces buecherae AC541<sup>T</sup> (99.2%), followed by Streptomyces rapamycinicus NRRL B-5491<sup>T</sup> (99.1%), Streptomyces luteoverticillatus NBRC 3840<sup>T</sup> (99.1%), Streptomyces coerulescens NBRC 12758<sup>T</sup> (99.1%), and Streptomyces iranensis HM 35<sup>T</sup> (99.0%). Strain CA3R110<sup>T</sup> contained LL-diaminopimelic acid in cell peptidoglycan, MK-9(H<inf>6</inf>), and MK-9(H<inf>8</inf>) as major menaquinone, iso-C<inf>16:0,</inf>iso-C<inf>15:0,</inf> C<inf>16:0</inf> as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside were detected in the cell. The chemotaxonomic characteristics possessed the typical properties of the genus Streptomyces. A low digital DNA–DNA hybridization (< 55.7%) and average nucleotide identity-blast (ANIb) (< 92.2%) values revealed that strain CA3R110<sup>T</sup> could be distinguished from any known Streptomyces species. With the differences in phenotypic and genotypic data, strain CA3R110<sup>T</sup> represents a novel species of genus Streptomyces, for which the name Streptomyces endocoffeicus sp. nov. is proposed. The type strain is CA3R110<sup>T</sup> (= TBRC 11245<sup>T</sup> = NBRC 114296<sup>T</sup>).
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    Streptomyces coffeae sp. Nov., an endophytic actinomycete isolated from the root of coffea arabica (l.)
    (2021-01-01)
    Nammali, Achararak
    ;
    Intaraudom, Chakapong
    ;
    Pittayakhajonwut, Pattama
    ;
    Suriyachadkun, Chanwit
    ;
    Tadtong, Sarin
    An endophytic actinobacterium, designated strain CA1R205<sup>T</sup>, was isolated from the surface-sterilized root of Coffea arabica L. collected from Ratchaburi province, Thailand. The taxonomic position of this strain was evaluated using a polyphasic approach. The strain produced light yellowish brown to dark brownish black substrate mycelium and greyish white aerial mycelium. The spiral spore chains were produced directly on aerial mycelium. CA1R205<sup>T</sup> was found to have ll-diaminopimelic acid in the cell peptidoglycan, galactose, glucose, mannose and ribose as whole-cell reducing sugars, MK-10(H<inf>4</inf>), MK-9(H<inf>6</inf>), MK-10(H<inf>2</inf>), MK-9(H<inf>4</inf>), MK-10(H<inf>6</inf>) and MK-10(H<inf>8</inf>) as menaquinones and iso-C<inf>15:0</inf>, anteiso-C<inf>15:0</inf>, iso-C<inf>16:0</inf> and C<inf>16:0</inf> as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were detected in the cells. These characteristics were consistent the typical chemotaxonomic properties of members the genus Streptomyces. The taxonomic affiliation at the genus level of this strain could be confirmed using its 16S rRNA gene sequence data. CA1R205<sup>T</sup> showed the highest 16S rRNA gene sequence similarity value to Streptomyces rapamycinicus NRRL B-5491<sup>T</sup> (98.9%), followed by Streptomyces iranensis HM 35<sup>T</sup> (98.8%). Digital DNA–DNA hybridization and average nucleotide identity-by blast (ANIb) values between CA1R205<sup>T</sup> and S. rapamycinicus NRRL B-5491<sup>T</sup> were 27.2 and 81.5%, respectively. The DNA G+C content of genomic DNA was 70.7 mol%. Due to the differences in physiological, biochemical and genotypic data, CA1R205<sup>T</sup> could be discriminated from its closest neighbour. Thus, CA1R205<sup>T</sup> should be recognized as representing a novel species of the genus Streptomyces, for which the name Streptomyces coffeae sp. nov. is proposed. The type strain is CA1R205<sup>T</sup> (=TBRC 11244<sup>T</sup>=NBRC 114295<sup>T</sup>).
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    Streptomyces musisoli sp. nov., an actinomycete isolated from soil
    (2021-01-01)
    Duangupama, Thitikorn
    ;
    Intaraudom, Chakapong
    ;
    Pittayakhajonwut, Pattama
    ;
    Suriyachadkun, Chanwit
    ;
    Tadtong, Sarin
    An actinobacterium, strain CH5-8<sup>T</sup>, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp. The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium. The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8<sup>T</sup>, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H<inf>8</inf>), MK-9(H<inf>6</inf>), and MK-9(H<inf>4</inf>) as major menaquinones and anteiso-C<inf>15:0</inf>, iso-C<inf>16:0</inf>, and anteiso-C<inf>17:0</inf> as major fatty acids. Diphosphatidylglycerol, phosphatidylethanola-mine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells. A combination of morphological and chemotaxonomic data supported the assignment to the genus Strep-tomyces. The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain. The novel strain CH5-8<sup>T</sup> showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763<sup>T</sup> (98.9%), followed by Streptomyces actinomycinicus RCU-197<sup>T</sup> (98.9%). The average nucleotide identity by blast (ANIb) and digital DNA–DNA hybridization values between CH5-8<sup>T</sup> and its closest relatives, S. echinatus CECT 3313<sup>T</sup> and S. actinomycinicus RCU-197<sup>T</sup>, were ≤91.6% and ≤47.4%, respectively. The digital DNA G+C content of genomic DNA was 72.1 mol%. On the basis of these phenotypic and genotypic data, strain CH5-8<sup>T</sup> represents a novel species, for which the name Streptomyces musisoli sp. nov. is proposed. The type strain is CH5-8<sup>T</sup> (=TBRC 9950<sup>T</sup>=NBRC 113997<sup>T</sup>).
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    Jiangella endophytica sp. nov., an endophytic actinomycete isolated from the rhizome of Kaempferia elegans
    (2019-02-01)
    Niemhom, Nantawan
    ;
    Chutrakul, Chanikul
    ;
    Suriyachadkun, Chanwit
    ;
    Tadtong, Sarin
    ;
    Thawai, Chitti
    An endophytic actinobacterium, designated strain KE2-3 <sup>T</sup> , was isolated from surface-sterilised rhizome of Kaempferia elegans. The polyphasic approach was used for evaluating the taxonomic position of this strain. The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of LL-diaminopimelic acid in the cell peptidoglycan, MK-9(H <inf>4</inf> ) as the major menaquinone, iso-C <inf>16: 0</inf> , anteiso-C <inf>15: 0</inf> , iso-C <inf>14: 0</inf> and iso-C <inf>15: 0</inf> as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes. Based on 16S rRNA gene sequence analysis, strain KE2-3 <sup>T</sup> was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357 <sup>T</sup> (99.3 %) followed by Jiangella alba DSM 45237 <sup>T</sup> (99.2 %), Jiangella alkaliphiliaDSM 45079 <sup>T</sup> (99.0 %), Jiangella gansuensis DSM 44835 <sup>T</sup> (98.8 %) and Jiangella mangrovi 3SM4-07 <sup>T</sup> (98.6 %). However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96%species circumscription threshold. The DNA G+C content of genomic DNA was 72.3 mol%. With the differences of physiological, biochemical and genotypic data, strain KE2-3 <sup>T</sup> could be discriminated from its closest neighbour. Thus, strain KE2-3 <sup>T</sup> should be recognised as a novel species of genus Jiangella, for which the name Jiangella endophytica sp. nov. is proposed. The type strain is KE2-3 <sup>T</sup> (=BCC 66359 <sup>T</sup> =NBRC 110004 <sup>T</sup> ).