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Author: search.filters.author.Tanasawet, SupitaHas files: No

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    Genome and transcriptome mining of the corazonin (Crz)-like peptide and gonadotropin-releasing hormone (GnRH)-like peptides in the spotted Babylon, Babylonia areolata
    (2026-06-01)
    Saetan, Uraipan
    ;
    Kornthong, Napamanee
    ;
    Duangprom, Supawadee
    ;
    Tanasawet, Supita
    ;
    Sukketsiri, Wanida
    The Spotted Babylon (Babylonia areolata) is an economically marine species in Thailand and other Asian countries. Due to the high demand for its meat, improving aquaculture practices for this species is essential. Regarding the lack of neuroendocrine control of reproduction in this species, we performed transcriptome analysis of the central nervous system and ovaries, and we searched against the reported genome of this animal species to find out the neurohormones. Here, we reported the identification of corazonin (ba-Crz)-like and gonadotropin-releasing hormone (ba-GnRH)-like mRNAs in B. areolata. The ba-Crz-like mRNA encoded mature ba-Crz-like peptide as QNYHYSNGWHP. Two ba-GnRH-like mRNAs encoded ba-GnRH-I-, and ba-GnRH-II-like peptides containing active peptides as QIHFSPTWGT and QIHFSHSWGT. Two introns were found in the ba-Crz-like gene while one intron was present in each ba-GnRH-like gene. These three peptides were phylogenetically placed in the molluscan Crz and GnRH clades. RT-PCR of these three mRNAs revealed their ubiquitous expressions across various organs, with all three predominantly expressed in ganglia, which was further confirmed by in situ hybridization of the cerebral ganglia. Immunohistochemistry showed positive signals for the Crz-like peptide in both the CNS and ovaries. To examine expression across ovarian cycle, the ba-Crz-like, and ba-GnRH-I-like mRNAs were consistently expressed in the CNS and ovaries of both immature and mature female snails, while the ba-GnRH-II-like mRNA significantly reduced its expression in the CNS of mature snails. Conclusively, this study was preliminary to report on the existence of GnRH/AKH/Crz peptides in the B. areolata, Further characterization of their receptors and biological functions is ongoing to ensure the GnRH, AKH, and Crz identification in this species.
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    Effects of cuttlebone powders and their structured calcium compounds against glucocorticoid-induced osteoporosis via MAPK signaling-mediated bone formation
    (2026-04-01)
    Woonnoi, Wanwipha
    ;
    Chinfak, Narainrit
    ;
    Tanasawet, Supita
    ;
    Saetan, Jirawat
    ;
    Klaypradit, Wanwimol
    The effects of dried and calcined cuttlebone powders (DCB and CCB, respectively) and their structured calcium compounds (DCB-OH, DCB-Lac, DCB-CiMa, CCB-OH, CCB-Lac, and CCB-CiMa) on MC3T3-E1 osteoblasts were investigated in vitro . Cytotoxicity analysis using the MTT assay revealed that lower concentrations (10–100 μg/mL) of all compounds were non-toxic, whereas higher concentrations (250–1000 μg/mL) showed dose-dependent cytotoxic effects. Using dexamethasone-induced osteoporosis-like MC3T3-E1 cells, cuttlebone-derived compounds significantly enhanced matrix mineralization as well as alkaline phosphatase (ALP) mRNA expression and activity, indicating restoration of osteoblast function. Additionally, treatment with all cuttlebone compounds reduced dexamethasone-induced apoptosis, demonstrating cytoprotective effects. Gene expression analysis showed suppression of ER stress markers ( CHOP, GRP78, ATF4 ) by most compounds, suggesting attenuation of ER stress-related osteoblast dysfunction. Osteoblast-related genes linked to matrix remodeling and bone formation ( MMP2, MMP9, COL1A1 ) were modulated; treatments generally downregulated MMP2 and upregulated COL1A1 expression. Key osteogenic transcription factors and markers ( BMP4, OC, OSX, RUNX2 ) decreased by dexamethasone were significantly upregulated by cuttlebone compounds, supporting enhanced osteoblast differentiation. Protein phosphorylation studies revealed that these compounds decreased the expression of pERK and p-p38, pathways that are often associated with impaired osteogenesis under glucocorticoid stress. Collectively, these findings demonstrate that cuttlebone powders and their structured calcium compounds mitigate dexamethasone-induced osteoporotic changes in MC3T3-E1 cells by enhancing cell viability, promoting osteoblast differentiation, and reducing apoptosis and ER stress, effects that are associated with suppression of MAPK pathway activation. This finding suggests a possible role in alleviating glucocorticoid-induced dysfunction in MC3T3-E1 osteoblasts and indicates potential relevance for future development as functional food ingredients or nutraceuticals for bone health.