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A smartphone-integrated rapid and sensitive lateral flow test strip for quantitative detection of Fusarium spp. in maize samples
(2025-05-01)
Rotamporn, Saowalak
;
Cheubong, Chehasan
;
Sansenya, Sompong
;
Jantra, Jongjit
;
Teepoo, Siriwan
In the world, maize is considered as one of the most valuable resources. Fusarium spp. are the primary soilborne plant-pathogenic fungi that can infect maize seeds and cause diseases that potentially decrease the production of crops. This study aims to develop a rapid and sensitive lateral flow test strip (LFTS) utilizing gold nanoparticles (AuNPs) for on-site detection of Fusarium spp. in maize samples. The smartphone-integrated LFTS enables the identification of Fusarium spp. within a concentration range of 0.2–1.5 nM and achieves a limit of detection (LOD) as low as 0.063 nM. The quantitative determination of the Fusarium spp. can be completed within 8 min. Maize samples were analyzed using both the LFTS and real-time polymerase chain reaction (real-time PCR). The LFTS demonstrated effective recovery in spiked samples, with values ranging from 80.1% to 101.1%. Furthermore, the real-time PCR results exhibited significant agreement with the LFTS results. Compared to the traditional real-time PCR technique, the proposed LFTS offers a sensitive, accurate, and rapid assay for the on-site detection of Fusarium spp. in maize samples.
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Smartphone-based imaging colorimetric assay for monitoring the quality of curcumin in turmeric powder
(2024-07-01)
Jantra, Jongjit
;
Teepoo, Siriwan
;
Thananimit, Suchera
This research developed a colorimetric assay for semi-quantitative curcumin detection. The screening test was performed using a ferric chloride to form a brownish color which was further used to evaluate the amount of curcumin in the turmeric powder samples. The quantitative assay was performed based on the color intensity of the curcumin target using a smartphone digital image colorimetry with a developed lightbox constructed with a white light-emitting diodes (LED) light source as the measurement device. Images in red, green, and blue (RGB) color were processed to obtain relevant colors from the image and the color values were used to analyze curcumin concentrations. The intensity of the ΔB was correlated to the concentration of curcumin with high sensitivity. The method showed a linear range between 0.25 and 5 mg L−1 with the LOD and LOQ of 0.12 and 0.41 mg L−1, respectively. Sample analysis was carried out in turmeric powders. Curcumin in turmeric powder samples was simply extracted using acetonitrile followed by dilution 100 times for sample preparation. The accuracy was tested by spiking 0.25, 1.00, and 4.00 mg L−1 of standard curcumin into the turmeric sample solution. The average percentage recoveries were acceptable in all samples (90–104%). The method was validated by comparing the results obtained from the proposed method and high-performance liquid chromatography (HPLC). There was no statistically significant difference between the two methods (P = 0.05). Graphical Abstract: (Figure presented.).
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Silica nanoparticle–modified paper strip–based new rhodamine B chemosensor for highly selective detection of copper ions in drinking water
(2023-08-01)
Sannok, Tadcha
;
Wechakorn, Kanokorn
;
Jantra, Jongjit
;
Kaewchoay, Netnapit
;
Teepoo, Siriwan
A new rhodamine B derivative (RDB) was synthesized and utilized for the colorimetric detection of copper ions (Cu2+). This chemosensor utilized a paper strip as a support and a smartphone as a detector for on-site quantitative detection of Cu2+ in water samples. Silica nanoparticles (SiNPs) were investigated as the modifier nanoparticles to achieve uniform color on the paper strip and showed a color response 1.9-fold higher than the one without SiNPs. The RDB chemosensor-based paper strip provided high selectivity toward Cu2+ with a detection limit of 0.7 mg/L, and the working concentrations for Cu2+ ranged from 1 to 17 mg/L. Parallel analyses of eight drinking water samples were conducted by inductively coupled plasma optical emission spectroscopy. The results were in good agreement, indicating the practical reliability of the established method with a short assay time and high selectivity. These indicate its great potential for on-site detection of Cu2+. Graphical abstract: [Figure not available: see fulltext.]
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Colorimetric test strip cassette readout with a smartphone for on-site and rapid screening test of carbamate pesticides in vegetables
(2022-10-01)
Rakkhun, Waraporn
;
Jantra, Jongjit
;
Cheubong, Chehasan
;
Teepoo, Siriwan
A test strip cassette integrated with a smartphone detector was developed for the colorimetric detection of carbamate pesticides in vegetables. The influence of various experimental conditions was examined for the detection of the optimum analytical performance of the test strip cassette. The test strip cassette detection of carbamate pesticides was carried out by using 140 mM of acetylthiocholine, 13 mM of 5,5′-dithiobis (2-nitrobenzoic acid), temperature at 37 °C and pH of 8.0. The developed test strip cassette platform is rapid within 5 min. Based on the inhibition effect of carbaryl on acetylcholinesterase activity, the inhibition effect of carbaryl was proportional to its concentration ranging from 1 to 50 mg/L with the detection limit of 0.30 mg/L. The limit of quantitative value was determined to be 1.0 mg/L. The spiking carbaryl, the recoveries were within 96–103% and the relative standard deviations were less than 1.56%. The analysis of carbamate pesticides residual in vegetable samples gave the results in good agreement with the most widely used commercial test kit and UPLC-MS/MS method. The developed test strip cassette required only 5 min for an assay. With the simple procedures and tiny size, it showed a great promising for the developed test strip to work on-site for carbamate pesticides detection in vegetables.

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