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    Postharvest detection of anthracnose (Colletotrichum asianum) on mango fruit (Mangifera indica L. cv Namdokmai Sithong) using near-infrared response
    (2026-12-01)
    Junto, Apiwat
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    Phanomsophon, Thitima
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    Sharma, Sneha
    ;
    Kaewsorn, Kannapot
    ;
    Jongyingcharoen, Jiraporn Sripinyowanich
    Anthracnose disease, caused by fungi of the genus Colletotrichum, poses a major threat to mango production and export industries, with Colletotrichum asianum being among the most significant pathogenic species. This work proposes the hypothesis that the simple difference in absorption between anthracnose-infected and noninfected mangoes illustrated by the average near-infrared (NIR) spectra obtained from hyperspectral images could be used for simple differentiation of the two groups. The method of depositing fungal spores by spraying the spores over the fruit surface, not a small area or specific point, allows for the number of spores per unit area to be harmonized and to detect infected or noninfected spores on every pixel of the mango surface using a hyperspectral imaging camera. Important wavelengths for differentiation included water bands of 970, 1190, and 1200 nm which resulted in the greatest difference in absorbance, and bands of chitin, the major component of the fungal cell wall; 1195 nm was the most important band. In addition, the vibration bands of 868 (protein in the fungal cell wall), 1134 (sugar and starch of the mango substrate), 1320 (NIR absorbers in the fungus-sprayed and mango substrate, not specifically defined) and 1069 nm (crystallinity and N-acetyl methyl groups in the fungal chitin and constituents of the mango), differed from each other. These wavelengths can be used for modelling, which can lead to high performance in quantifying the concentration of anthracnose and classifying the strength levels of anthracnose infection. The microbiological mechanism of anthracnose growth on infected mangoes corresponding to changes in the NIR spectrum during the 4 days after spore infection is comprehensively discussed. These results can aid in enhancing early detection and classification techniques for anthracnose-infected mangoes from noninfected mangoes using hyperspectral image sensors.
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    Item type:Publication,
    Filter-based short-wave infrared imaging combined with machine learning for non-destructive quality assessment of durian pulp
    (2026-09-01)
    Promnioy, Surasak
    ;
    Phetpan, Kittisak
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    Riza, Dimas Firmanda Al
    ;
    Sharma, Sneha
    ;
    Sirisomboon, Panmanas
    The development of affordable, real-time quality monitoring tools is essential for industrial applications involving high-value tropical fruits such as durian. This study presents a cost-effective short-wave infrared multispectral imaging (SWIR-MSI) system employing three discrete bandpass filters (880, 905, and 940 nm) integrated with machine learning algorithms for non-destructive evaluation of fresh-cut durian pulp. Compared with conventional point-based NIR spectroscopy and complex hyperspectral imaging systems, the proposed configuration markedly reduces system complexity and cost while maintaining sensitivity to key compositional variations. It accurately predicted dry matter content (DMC) and starch, demonstrating that limited spectral information within the 860–1100 nm range can effectively capture moisture- and carbohydrate-related features. These findings confirm the feasibility of implementing filter-based SWIR imaging as a practical and scalable alternative to hyperspectral systems for on-line fruit quality assessment. Practically, this approach enables rapid, non-destructive, and spatially adaptable evaluation of durian pulp quality, offering significant potential for on-site grading, ripeness classification, and process control in fresh-cut durian production and packaging operations, particularly for small- and medium-scale agro-processors.
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    Transient heat modeling for non-destructive assessment of boiled eggs
    (2023-10-01)
    Sahachairungrueng, Woranitta
    ;
    Tonpho, Pasika
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    Veeradechakul, Mungkarej
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    Rosnim, Thitirat
    ;
    Thompson, Anthony Keith
    It is not possible to differentiate between hard-boiled and soft-boiled eggs after processing. Therefore, transient heat of boiled eggs was tested during the production process in order to develop a model that could be used to differentiate between soft-boiled and hard-boiled eggs. Both types of boiled eggs (N=214) were produced in water at 90 °C for different times and then cooled down. The temperature gradients due to heat transfer during cooling in the ambient air were measured every 30 seconds. Results showed that a dimensionless parameter, called number of transfer units (NTU), changed in relation to time during the cooling process, and it was shown that this could be used as an independent variable. Classification models were established using linear discriminant analysis (LDA) and support vector machine classification (SVMC). Samples were divided into a calibration set (N=150) and a prediction set (N=64). The predictive accuracy of the models using LDA and SVMC for classifying eggs into soft-boiled or hard-boiled was 93.8% and 92.2%, respectively. Therefore, it was concluded that the classification models using LDA and SVMC had potential for use as a non-destructive method for classifying groups of eggs into soft-boiled and hard-boiled that could potentially be used in a commercial situation.
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    Non-Destructive Classification of Organic and Conventional Hens’ Eggs Using Near-Infrared Hyperspectral Imaging
    (2023-07-01)
    Sahachairungrueng, Woranitta
    ;
    Thompson, Anthony Keith
    ;
    Terdwongworakul, Anupun
    ;
    Teerachaichayut, Sontisuk
    Eggs that are produced using organic methods retail at higher prices than those produced using conventional methods, but they cannot be differentiated reliably using visual methods. Eggs can therefore be fraudulently mislabeled in order to increase their wholesale and retail prices. The objective of this research was therefore to test near-infrared hyperspectral imaging (NIR-HSI) to identify whether an egg has been produced using organic or conventional methods. A total of 210 organic and 210 conventional fresh eggs were individually scanned using NIR-HSI to obtain absorbance spectra for discrimination analysis. The physical properties of each egg were also measured non-destructively in order to analyze the performance of discrimination compared with those of the NIR-HSI spectral data. Principal component analysis (PCA) showed variation for PC1 and PC2 of 57% and 23% and 94% and 4% based on physical properties and the spectral data, respectively. The best results of the classification using NIR-HSI spectral data obtained an accuracy of 96.03% and an error rate of 3.97% via partial least squares–discriminant analysis (PLS-DA), indicating the possibility that NIR-HSI could be successfully used to rapidly, reliably, and non-destructively differentiate between eggs that had been produced using organic methods from eggs that had been produced using conventional methods.
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    Assessment of Nitrite Content in Vienna Chicken Sausages Using Near-Infrared Hyperspectral Imaging
    (2023-07-01)
    Tantinantrakun, Achiraya
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    Thompson, Anthony Keith
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    Terdwongworakul, Anupun
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    Teerachaichayut, Sontisuk
    Sodium nitrite is a food additive commonly used in sausages, but legally, the unsafe levels of nitrite in sausage should be less than 80 mg/kg, since higher levels can be harmful to consumers. Consumers must rely on processors to conform to these levels. Therefore, the determination of nitrite content in chicken sausages using near infrared hyperspectral imaging (NIR-HSI) was investigated. A total of 140 chicken sausage samples were produced by adding sodium nitrite in various levels. The samples were divided into a calibration set (n = 94) and a prediction set (n = 46). Quantitative analysis, to detect nitrate in the sausages, and qualitative analysis, to classify nitrite levels, were undertaken in order to evaluate whether individual sausages had safe levels or non-safe levels of nitrite. NIR-HSI was preprocessed to obtain the optimum conditions for establishing the models. The results showed that the model from the partial least squares regression (PLSR) gave the most reliable performance, with a coefficient of determination of prediction (R<inf>p</inf>) of 0.92 and a root mean square error of prediction (RMSEP) of 15.603 mg/kg. The results of the classification using the partial least square-discriminant analysis (PLS-DA) showed a satisfied accuracy for prediction of 91.30%. It was therefore concluded that they were sufficiently accurate for screening and that NIR-HSI has the potential to be used for the fast, accurate and reliable assessment of nitrite content in chicken sausages.
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    Item type:Publication,
    Near-infrared hyperspectral imaging combined with machine learning for physicochemical-based quality evaluation of durian pulp
    (2023-06-01)
    Sharma, Sneha
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    Sirisomboon, Panmanas
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    K.C, Sumesh
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    Terdwongworakul, Anupun
    ;
    Phetpan, Kittisak
    This research reports on the application of near-infrared hyperspectral imaging (NIR-HSI) system for predicting the physicochemical properties; dry matter (DM), total soluble solids (TSS), and fat content (FC) of durian. Partial least squares regression (PLSR), support vector machine (SVM), random forest (RF), and 1D convolution neural network (CNN) models: custom, U-Net, and VGG19; were developed to predict DM, TSS, and FC of durian pulp. Feature wavelengths were selected using a genetic algorithm (GA) and successive projection algorithm (SPA). The selected wavelengths were then validated based on the algorithms for regression model development. GA-PLSR model was compelling to predict the DM and FC in durian pulp, which obtained the coefficient of determination for the test set (r<sup>2</sup>) and root mean square error of prediction (RMSEP) of 0.97 and 1.12% for DM and 0.86 and 0.64% for FC, respectively. The GA-PLSR model provided the best result for the TSS prediction with r<sup>2</sup>, and RMSEP of 0.90 and 1.40%, respectively, whereas the SPA-PLSR model based on only thirteen wavelengths attained fair result with the r<sup>2</sup> and RMSEP of 0.79 and 2.03%, respectively. The above results show that the pushbroom NIR-HSI system achieved promising results for estimating DM, TSS, and FC in durian pulp. This research identified the featured wavelengths that can be used to develop a portable and reliable HSI or multispectral system to be installed at durian packaging firms for quality inspection and grading.
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    Item type:Publication,
    Improving the non-destructive maturity classification model for durian fruit using near-infrared spectroscopy
    (2023-03-01)
    Ditcharoen, Sirirak
    ;
    Sirisomboon, Panmanas
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    Saengprachatanarug, Khwantri
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    Phuphaphud, Arthit
    ;
    Rittiron, Ronnarit
    The maturity state of durian fruit is a key indicator of quality before trading. This research aims to improve the near-infrared (NIR) model for classifying the maturity stage of durian fruit using a completely non-destructive measurement. Both NIR spectrometers were investigated: the short wavelength NIR (SWNIR) ranging from 450 to 1000 nm and long wavelength NIR (LWNIR) ranging from 860 to 1750 nm. The samples collected for experimentation consisted of four stages: immaturity, prematurity, maturity, and ripe. Each fruit was scanned at the rind position on the main fertile lobe (header, middle, and tail) and stem. The classification models were developed using three supervised machine learning algorithms: linear discriminant analysis (LDA), support vector machine (SVM), and K-Nearest neighbours (KNN). The analysis results revealed that the use of durian rind spectra only obtained between 83.15% and 88.04% accuracy for the LWNIR spectrometer, while the SWNIR spectrometer provided 64.73 to 93.77% accuracy. The performance of model increases when developing with combination between rind and stem spectra. The LDA model developed using a combination of rind and stem spectra provided the greatest efficiency, exhibiting 97.28% and 100% accuracy for LWNIR and SWNIR spectrometers, respectively. The LDA model is therefore recommended for obtaining spectra from smoothing moving average (MA) + baseline of rind position and when used in combination with the MA + standard normal variance (SNV) of stem spectra. The NIR spectroscopy indicated high potential for non-destructive estimation of the durian maturity stage. This process could be used for quality control in the durian export industry to solve the problem of unripe durian being mixed with ripe fruit.
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    Minimally destructive assessment of mangosteen translucency based on electrical impedance measurements
    (2016-02-01)
    Nakawajana, Natrapee
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    Terdwongworakul, Anupun
    ;
    Teerachaichayut, Sontisuk
    Electrical impedance spectroscopy in a frequency range of 1 kHz-200 kHz was studied to develop a classifying model for translucent mangosteen. The optimal configuration of the measurement was investigated. Transverse alignment of two measuring needles with the stem-calyx axis and with the measured position on the part of the pericarp pertinent to the largest flesh segment proved to be the optimal configuration. The optimal electrical parameters were selected at frequencies of 1, 4, 7, 8, 14, 47, 73, and 81 kHz as the classifying variables based on the student t-test analysis for a significant difference between the normal and translucent mangosteen and the largest difference of the average values of the electrical parameters. The differences in the electrical parameters and their reciprocals were the optimal classifying variables. The model constructed from the samples from two seasons was robust in terms of seasonality, providing a classification accuracy of 82.7%. The difference in the initial moisture content of the pericarp was justifiably compensated by the differences in the electrical parameters. The EIS technique was suitable for measurement of mangosteen samples at the maturity color stage in which the sample contained no yellow latex in the pericarp.
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    Quantitative prediction of nitrate level in intact pineapple using Vis-NIRS
    (2015-01-01)
    Srivichien, Sasathorn
    ;
    Terdwongworakul, Anupun
    ;
    Teerachaichayut, Sontisuk
    Before pineapples are canned, the ones with high nitrate level must be sorted out first because nitrate causes black stains on the surface of the can; therefore, a nondestructive technique for sorting out pineapples is clearly needed. The use of visible and near infrared (Vis-NIR) spectroscopy for such purpose was investigated in this study. A batch of 75 pineapple fruits that would have been delivered to a canning factory was tested. Spectra were acquired using a spectrophotometer in interactance mode with wavelengths in the region of 400-2500 nm. Twelve scans of different parts of each pineapple were made. The actual amount of nitrate in the pineapple flesh was determined by HPLC. Original spectra and pretreated spectra were both used to construct calibration models with partial least squares regression (PLSR). The best model was obtained from an average spectrum pretreated with first derivative treatment at the wavelength range of 600-1200 nm. Predictions based on this model matched closely with the actual nitrate contents, with a high correlation coefficient (R) of 0.95 and a low root mean square error of prediction (RMSEP) of 1.77 ppm. These results demonstrate that Vis-NIR spectroscopy can be used for rough screening of intact pineapple.
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    Determination of translucent content in mangosteen by means of near infrared transmittance
    (2012-03-01)
    Terdwongworakul, Anupun
    ;
    Nakawajana, Natrapee
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    Teerachaichayut, Sontisuk
    ;
    Janhiran, Athit
    Translucent flesh disorder is undesirable in mangosteen meant for export. However, mangosteens are judged as translucent when the translucent flesh is visible on the pulp surface regardless of the quantity of the internal translucent flesh which may result in some mangosteen assessed as normal having the same amount of translucent flesh content as a mangosteen judged as translucent. The critical amount of translucent flesh to be visible on the pulp surface needs to be determined for assessment purposes. A non-destructive technique to measure the translucent content is a practical tool as the first step towards the establishment of the critical value. A non-destructive model was developed to estimate the translucent content in mangosteens using near infrared transmittance. The translucent area of the flesh section on the fruit surface was used to indicate the translucent content. The effects of the orientation of the fruit and also of the light source to the relative position of the detector as well as the effect of the measurement position of the fruit on the predictive performance were examined. The results showed that the best partial least squares model was achieved with spectra acquired from the fruit position which revealed the largest flesh segment (prediction correlation coefficient was 0.86 and root mean square error of prediction was 7.58%). The horizontal stem-calyx fruit axis and a 135° angle from the light source relative to the detector were the optimal fruit orientation and configuration for measurement. © 2011 Elsevier Ltd. All rights reserved.