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Author: search.filters.author.Thawai, ChittiSubject: search.filters.subject.Antimicrobial

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    Antimalarial and antimicrobial substances isolated from the endophytic actinomycete, Streptomyces aculeolatus MS1-6
    (2023-03-01)
    Kuncharoen, Nattakorn
    ;
    Bunbamrung, Nantiya
    ;
    Intaraudom, Chakapong
    ;
    Choowong, Wilunda
    ;
    Thawai, Chitti
    Seven undescribed compounds, including four naphthoquinone terpenoids (aculeolatins A – D), one rare 2-nitropyrrole terpenoid (nitropyrrolin F), and two hydroxamate siderophores (aculeolamides A and B) and one further undescribed compound (2,5,7-trihydroxy-3,6-dimethylnaphthalene-1,4-dione), together with eleven known compounds (arromycin, phenaziterpene A, nitropyrrolin A, heronapyrroles A and B, salaceyin A, 5,7-dihydroxy-2-isopropylchromone, 1-hydroxyphenazine, 1-methoxyphenazine, 1-acetyl-β-carboline, and N-(2-phenylethyl) acetamide), were isolated from the cultures of the endophytic Streptomyces aculeolatus MS1-6. The structures of the isolated compounds were determined using NMR spectroscopy and corroborated using chemical modification. These compounds exhibited a broad spectrum of biological activities, including antimalarial (IC<inf>50</inf> 6.03–9.84 μg/mL), antitubercular (MIC 3.13–6.25 μg/mL), anti-plant pathogenic fungal (MIC 25.0–50.0 μg/mL), and antibacterial (MIC 3.03–50 μg/mL) activities; however, they displayed unremarkable cytotoxicity against cancerous (MCF-7 and NCI–H187) and non-cancerous (Vero) cell lines.
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    Antimicrobial and anti-oxidant activities of quinoline alkaloids from Pseudomonas aeruginosa BCC76810
    (2016-09-01)
    Supong, Khomsan
    ;
    Thawai, Chitti
    ;
    Supothina, Sumalee
    ;
    Auncharoen, Patchanee
    ;
    Pittayakhajonwut, Pattama
    Twelve 4-hydroxyquinoline derivatives (1–12) and three phenazine alkaloids (13–15) have been isolated from Pseudomonas aeruginosa BCC76810. All these compounds, except compounds 6, 7, 9, and 11, are known. The new quinolines are 2-((Z)-undec-4′-enyl)-4-hydroxyquinoline (6), 2-(3′-(2′-hexylcyclopropyl)propyl)-4-hydroxyquinoline (7), 2-n-octyl-4-hydroxyquinoline N-oxide (9), and 2-((Z)-undec-4′-enyl)-4-hydroxyquinoline N-oxide (11). Their structures were elucidated based on the spectroscopic information, such as 1D, 2D NMR, UV, and HRESIMS data. 4-Hydroxyquinolines (1–6) and 4-hydroxyquinoline-N-oxides (8–11) exhibited antimalarial activity against Plasmodium falciparum, K1 strain (IC<inf>50</inf> 0.25–2.07 μg/mL) with moderate to weak cytotoxicity against cancerous (KB, MCF-7, NCI-H187) and non-cancerous (Vero) cells. In addition, 4-hydroxyquinoline N-oxides also displayed anti-Bacillus cereus and antioxidant activities.
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    Antimicrobial compounds from endophytic Streptomyces sp. BCC72023 isolated from rice (Oryza sativa L.)
    (2016-05-01)
    Supong, Khomsan
    ;
    Thawai, Chitti
    ;
    Choowong, Wilunda
    ;
    Kittiwongwattana, Chokchai
    ;
    Thanaboripat, Dusanee
    An endophytic actinomycete strain BCC72023 was isolated from rice (Oryza sativa L.) and identified as the genus Streptomyces, based on phenotypic, chemotaxonomic and 16S rRNA gene sequence analyses. The strain showed 99.80% similarity compared with Streptomyces samsunensis M1463<sup>T</sup>. Chemical investigation led to the isolation of three macrolides, efomycins M (1), G (2) and oxohygrolidin (3), along with two polyethers, abierixin (4) and 29-O-methylabierixin (5). To our knowledge, this is the first report of efomycin M being isolated from a natural source. The compounds were identified using spectroscopic techniques and comparison with previously published data. All compounds exhibited antimalarial activity against the Plasmodium falciparum, K-1 strain, a multidrug-resistant strain, with IC<inf>50</inf> values in a range of 1.40-5.23 μg/ml. In addition, these compounds were evaluated for biological activity against Mycobacterium tuberculosis, Bacillus cereus, Colletotrichum gloeosporioides and Colletotrichum capsici, as well as cytotoxicity against both cancerous (MCF-7, KB, NCI-H187) and non-cancerous (Vero) cells.