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Author: search.filters.author.Vatanavicharn, TipachaiSubject: search.filters.subject.Penaeus monodon

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    Role of cyclophilin A as an immunostimulator in responses to Vibrio parahaemolyticus AHPND infection in the black tiger shrimp Penaeus monodon
    (2025-11-01)
    Towittayapanchai, Pornsawan
    ;
    Ponprateep, Sirikwan
    ;
    Supungul, Premruethai
    ;
    Tang, Sureerat
    ;
    Jaree, Phattarunda
    Cyclophilin A (CypA) is a highly conserved peptidyl-prolyl isomerase that plays a crucial role in protein folding, immune regulation, and cellular stress responses. In shrimp, CypA has been implicated in innate immunity, particularly in response to viral and bacterial infections. This study investigates the role of the Penaeus monodon cyclophilin A (PmCypA) in shrimp infected with V. parahaemolyticus AHPND (VP<inf>AHPND</inf>). During VP<inf>AHPND</inf> infection, the expression levels of PmCypA gene and PmCypA protein were significantly increased at 24 h post-infection (h.p.i.) in shrimp hemocyte. To investigate the role of PmCypA in shrimp immune response, shrimp were administrated with the recombinant PmCypA protein (rPmCypA) and submerged in VP<inf>AHPND</inf> suspension. The results showed that the rPmCypA could rapidly stimulate the expression of immune-related genes involved in several immune pathways, such as immune recognition receptors, clotting system, phagocytosis and homeostasis. Moreover, the injected rPmCypA led to an increase in the proportion of hyaline cells and the greater quantity of granulocytes that contained PmCypA. Furthermore, the rPmCypA could prolong shrimp survival during VP<inf>AHPND</inf> infection. These results suggest that the PmCypA is a bacterial-responsive gene in shrimp immune defense response and potentially an immunostimulator in shrimp.
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    Antiviral properties of Penaeus monodon cyclophilin A in response to white spot syndrome virus infection in the black tiger shrimp
    (2024-01-01)
    Vatanavicharn, Tipachai
    ;
    Matjank, Watchalaya
    ;
    Masrinoul, Promsin
    ;
    Supungul, Premruethai
    ;
    Tassanakajon, Anchalee
    Cyclophilin A (CypA) or peptidylprolyl isomerase A, plays an important role in protein folding, trafficking, environmental stress, cell signaling and apoptosis etc. In shrimp, the mRNA expression level of PmCypA was stimulated by LPS. In this study, all three types of shrimp hemocytes: hyaline cell, granulocyte and semi-granulocyte expressed the PmCypA protein. The mRNA expression level of PmCypA was found to be up-regulate to four-fold in white spot syndrome virus (WSSV) infected hemocytes at 48 h. Interestingly, PmCypA protein was only detected extracellularly in shrimp plasma at 24 h post WSSV infection. To find out the function of extracellular PmCypA, the recombinant PmCypA (rPmCypA) was produced and administrated in shrimp primary hemocyte cell culture to observe the antiviral properties. In rPmCypA-administrated hemocyte cell culture, the mRNA transcripts of WSSV intermediate early gene, ie1 and early gene, wsv477 were significantly decreased but not that of late gene, vp28. To explore the antiviral mechanism of PmCypA, the expression of PmCypA in shrimp hemocytes was silenced and the expression of immune-related genes were investigated. Surprisingly, the suppression of PmCypA affected other gene expression, decreasing of penaeidin, PmHHAP and PmCaspase and increasing of C-type lectin. Our results suggested that the PmCypA might plays important role in anti-WSSV via apoptosis pathway. Further studies of PmCypA underlying antiviral mechanism are underway to show its biological function in shrimp immunity.
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    Plasmolipin, PmPLP1, from Penaeus monodon is a potential receptor for yellow head virus infection
    (2018-11-01)
    Matjank, Watchalaya
    ;
    Ponprateep, Sirikwan
    ;
    Rimphanitchayakit, Vichien
    ;
    Tassanakajon, Anchalee
    ;
    Somboonwiwat, Kunlaya
    Plasmolipin has been characterized as a cell entry receptor for mouse endogenous retrovirus. In black tiger shrimp, two isoforms of plasmolipin genes, PmPLP1 and PmPLP2, have been identified from the Penaeus monodon EST database. The PmPLP1 is highly up-regulated in yellow head virus (YHV)-infected shrimp. Herein, the function of PmPLP1 is shown to be involved in YHV infection. The immunoblotting and immunolocalization showed that the PmPLP1 protein was highly expressed and located at the plasma membrane of gills from YHV-infected shrimp. Moreover, the PmPLP1 expressed in the Sf9 insect cells resided at the cell membrane rendering the cells more susceptible to YHV infection. Using the ELISA binding and mortality assays, the synthetic external loop of PmPLP1 was shown to bind the purified YHV and neutralize the virus resulting in the decrease in YHV infection. Our results suggested that the PmPLP1 was likely a receptor of YHV in shrimp.
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    Two plasmolipins from the black tiger shrimp, Penaeus monodon and their response to virus pathogens
    (2012-01-01)
    Vatanavicharn, Tipachai
    ;
    Pongsomboon, Siriporn
    ;
    Tassanakajon, Anchalee
    Two isoforms of plasmolipin were initially identified from the black tiger shrimp (Penaeus monodon) EST database and completed using 5' RACE to reveal complete cDNAs of 558. bp (PmPLP1) and 537. bp (PmPLP2) with 87% nucleotide sequence identity. The deduced amino acid sequences contained four-transmembrane domains and showed the highest amino acid identity (49% and 51%, respectively) to the honey bee (Apis mellifera) chemokine-like factor (CKLF), with a very similar hydrophobic pattern to other plasmolipins. Transcripts of PmPLP1 and PmPLP2 were observed in all tested shrimp tissues with the highest expression levels in the gill and epipodite for PmPLP1 and in the hemocytes and antennal gland for PmPLP2. PmPLP1 transcript levels were significantly upregulated in hemocytes at 24 and 72. h post infection (hpi) with yellow head virus (YHV) (7.4- and 14.7- fold, respectively), but only after 72. hpi by white spot syndrome virus (WSSV). In contrast, PmPLP2 was only slightly (but statistically significant) up-regulated with YHV and WSSV. Thus, PmPLPs have the potential to be a part of viral infection mechanism or defense response. This is the first characterization of a plasmolipin gene in crustaceans. © 2012 Elsevier Ltd.