KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Settings

Author: search.filters.author.Visitsattapongse, SarinpornSubject: search.filters.subject.screen-printed electrode

Search Results

Now showing 1 - 3 of 3
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Ultrasensitive Label-Free Electrochemical Detection of Pseudomonas aeruginosa Using a Surface Molecularly Imprinted Polymer-Modified Screen-Printed Electrode
    (2026-06-01)
    Vongmanee, Naphatsawan
    ;
    Nampeng, Jindapa
    ;
    Pintavirooj, Chuchart
    ;
    Visitsattapongse, Sarinporn
    Pseudomonas aeruginosa is a major opportunistic pathogen frequently associated with nosocomial infections, such as pneumonia, urinary tract infections, and wound infections, particularly in immunocompromised or hospitalized patients. These infections are often difficult to treat due to the pathogen’s intrinsic antibiotic resistance and biofilm-forming ability. Therefore, rapid and selective detection of P. aeruginosa is essential for early diagnosis and effective infection control. In this study, a novel surface-imprinted MIP design uniquely combines methacrylamide (MAM), acrylamide (AAM), and vinylpyrrolidone (VP) monomers to generate recognition cavities that are complementary to the surface morphology and physicochemical properties of Pseudomonas aeruginosa cells. Unlike traditional MIP approaches, this surface imprinting strategy provides improved stability and reproducibility, without relying on biological recognition elements like antibodies or aptamers. This novel approach enabled us to achieve an ultralow LOD of 1 CFU/mL over a linear range of 1–10<sup>4</sup> CFU/mL, demonstrating excellent analytical performance. In addition, the sensor exhibited good reproducibility with an RSD of 5–12%. The novelty of this work lies in the use of a surface-imprinted MIP strategy combined with a multi-monomer system to enhance bacterial recognition and sensing performance. Overall, the proposed MIP-based electrochemical biomimetic sensor offers a rapid, cost-effective, and portable platform with strong potential for the detection of P. aeruginosa in clinical and environmental applications.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Electrochemical Characterization of a Molecularly Imprinted Polymer Sensor for the Selective Recognition of Type II Collagen in Joint Degeneration Monitoring
    (2026-02-01)
    Nampeng, Jindapa
    ;
    Vongmanee, Naphatsawan
    ;
    Pintavirooj, Chuchart
    ;
    Visitsattapongse, Sarinporn
    Type II collagen is a primary fibrillar component of articular cartilage, and its early degradation is a key biomarker of joint-degenerative disorders such as osteoarthritis, rheumatoid arthritis, gout, etc. Reliable detection at low concentrations remains challenging due to limited assay accessibility, complex analytical procedures, and nonspecific responses in multicomponent biological matrices. This research reports the development of a Molecularly Imprinted Polymer (MIP)–based electrochemical sensor engineered for the selective recognition of type II collagen. A series of monomer formulations were evaluated, and the 1AAM:2VP composition produced a well-defined imprinted layer on screen-printed carbon electrodes, yielding the highest electrochemical sensitivity and linearity. The optimized sensor exhibited strong anodic and cathodic responses proportional to increasing collagen concentrations, with a calibration slope corresponding to an R<sup>2</sup> value of 0.9394. Minimal signal interference was observed, confirming high molecular selectivity. The limit of detection (LOD) was calculated to be approximately 0.065 µg/mL. These characteristics demonstrate that the proposed MIP sensor provides a low-cost, accessible, and highly selective analytical platform suitable for early-stage cartilage degeneration monitoring.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Biosensor Based on Electrochemical Analysis for Staphylococcus aureus Detection with Molecular Imprinted Polymer Technique
    (2025-11-01)
    Vongmanee, Naphatsawan
    ;
    Nampeng, Jindapa
    ;
    Pintavirooj, Chuchart
    ;
    Visitsattapongse, Sarinporn
    Staphylococcus aureus (S. aureus) is one of the most common hospital-acquired pathogens and poses a serious threat to patients with weakened immune systems. Transmission can occur through foodborne illness, skin infections, abscess formation, and bloodstream invasion. The most severe complication arises when S. aureus infects the heart, leading to valve damage and potentially progressing to heart failure. In addition, many strains have developed strong resistance to conventional antibiotic therapies, making treatment increasingly difficult. These challenges highlight the importance of early detection for effective prevention and management. This research focuses on the development of a polymer composite incorporating hydroxyproline for the preparation of molecularly imprinted polymers (MIPs) designed for the rapid detection of S. aureus. The sensing platform, based on electrochemical principles, enabled sensitive and efficient analysis of bacterial samples. The sensor exhibited a broad analytical range, detecting S. aureus from 1 to 10,000 CFU/mL, with a detection limit as low as 1.031 CFU/mL. Selectivity testing against Pseudomonas aeruginosa, Candida albicans, and Escherichia coli confirmed high specificity toward S. aureus. These findings highlight the potential of this MIP-based electrochemical sensor as a reliable tool for rapid bacterial detection in clinical and environmental settings.