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Author: search.filters.author.Wanwong, SompitSubject: search.filters.subject.gallic acid

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    Electrospun Cellulose Acetate/Poly(Vinyl Alcohol) Nanofibers Loaded with Methyl Gallate and Gallic Acid for Anti-Staphylococcus aureus Applications
    (2024-11-01)
    Jiamboonsri, Pimsumon
    ;
    Sangkhun, Weradesh
    ;
    Wanwong, Sompit
    Methyl gallate (MG) and gallic acid (GA) are natural compounds with potent activity against methicillin-resistant Staphylococcus aureus (MRSA), a significant global health concern. In this study, MG and GA were incorporated into cellulose acetate (CA) blended with poly(vinyl alcohol) (PVA) to create electrospun nanofibers aimed at combating both methicillin-susceptible S. aureus (MSSA) and MRSA. Key electrospinning parameters—DC voltage, injection flow rate, and syringe tip–collector distance—were optimized, with the best conditions being a 1.5 mL/h flow rate, 30 cm distance, and 20 kV voltage. The resulting nanofiber mats were characterized by SEM, FTIR, DSC, tensile strength testing, contact angle measurement, swelling behavior, and release profiling. Antibacterial properties were assessed using the agar diffusion test. The obtained nanofibers had diameters ranging from 879.33 to 906.13 nm. Among the samples, MG-GA-CA/PVA exhibited the highest tensile strength, good flexibility, and improved stiffness, which was related to enhanced thermal stability and chemical interactions as shown by DSC and FTIR analyses. This formulation also displayed excellent hydrophilicity, swelling properties, and a consistent release profile over 8 to 24 h. Furthermore, MG-GA-CA/PVA showed superior antibacterial activity against both MSSA and MRSA, suggesting its potential as a strong, flexible, and effective anti-S. aureus material.
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    Assessing the Potential of Gallic Acid and Methyl Gallate to Enhance the Efficacy of β-Lactam Antibiotics against Methicillin-Resistant Staphylococcus aureus by Targeting β-Lactamase: In Silico and In Vitro Studies
    (2023-11-01)
    Jiamboonsri, Pimsumon
    ;
    Eurtivong, Chatchakorn
    ;
    Wanwong, Sompit
    Methicillin-resistant Staphylococcus aureus (MRSA), a global health concern, has prompted research into antibiotic adjuvants as a potential solution. Although our group previously reported the enhancing effects of gallic acid (GA) and methyl gallate (MG) on penicillin G activity against MRSA, the synergistic potential with other β-lactam antibiotics and the underlying mechanism have not been fully explored. Therefore, this study primarily aimed to investigate the antibacterial synergism with β-lactam antibiotics through disc diffusion, checkerboard, and time–kill assays. The β-lactamase inhibition was also examined through both molecular modeling and in vitro experiments. Additionally, bacterial morphology changes were studied using a scanning electron microscopy (SEM). The results revealed that both GA and MG exhibited anti-MRSA activity and showed indifferent effects when combined with β-lactam antibiotics against methicillin susceptible S. aureus (MSSA). Interestingly, MG demonstrated synergism with only the β-lactamase-unstable antibiotics against MRSA with the lowest fractional inhibitory concentration (FIC) indexes of ≤3.75. However, GA and MG exhibited weak β-lactamase inhibition. Furthermore, GA, MG, and the combination with ampicillin induced the morphological changes in MRSA, suggesting a possible mechanism affecting the cell membrane. These findings suggest that MG could potentially serve as an adjunct to β-lactam antibiotics to combat MRSA infections.