KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/kmitl/1

Browse

Settings

Start date: 2020Subject: search.filters.subject.Regeneration

Search Results

Now showing 1 - 2 of 2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Drought tolerance of Stylosanthes guianensis CIAT 184 by tissue culture
    (2026-05-01)
    Poeaim, S.
    ;
    Ngoenngam, L.
    ;
    Poeaim, A.
    The multiple shoots induction of Stylosanthes guianensis CIAT 184 was cultured on MS medium supplemented with 3 mg/l TDZ. The percentage of shoot induction was 100%, the average number of shoots was 36.80 per seed, and the average height of shoots was 0.76 cm. The shoots were transferred to MS medium for rooting, supplemented with 0.3 mg/l IAA. The percentage of root induction was 40%, and the average number of roots was 6.50 per shoot. Physical gamma rays induced the mutation. Seeds were irradiated with 0, 5, 10, 15, 20, 25, 30 and 50 Krad. The results showed that S. guianensis CIAT 184 irradiated at 44.38 Krad gave 50 % (GR50(30)) and 50% LD50(30) of 38.06 Krad. The drought tolerance of seeds supplemented with 0, 5, 10, 15 and 20% of PEG-6000. The GR50 was 17.73 % at 2 weeks, and the LD50 was 14.86 % and 11.83 % for 4 and 12 weeks, respectively.
      1
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Effects of 6 benzylaminopurine and meta topoline on micropropagation of Dendrobium chrysanthum Wall. ex. Lindl
    (2022-09-01)
    Poeaim, A.
    ;
    Kunwanlop, W.
    ;
    Boonmee, W.
    ;
    Laipasu, P.
    The results of solid Gamborg's B5 medium at 2 mg/L mT found the highest average number of shoots at 1.6 shoots per explant; and the number of leaves at 3.7 leaves per explant. The concentration of 1.5 mg/L of mT showed the highest average length of shoots which was 9.82 mm, while the 0.5 mg/L mT revealed the most significant number of roots which were 0.9 roots per explant with the longest root of 3.75 mm. The liquid Gamborg's B5 medium showed the highest average number of shoots at 1.5 mg/L BAP, the same as at 1.5 mg/L mT. The medium without plant growth regulator and 1 mg/L mT was the highest average length of shoots of 13.98 mm. The highest number of roots per explant was 0.8, similar to 2 mg/L mT, but the different maximum length of roots of 3.04 mm. A hundred percent of protocorms induced shoots found in all media. The plantlets were survived after transplanting into pots and covered with plastic bags in four weeks.
      1