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Start date: 2020Subject: search.filters.subject.Secondary metabolite

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Now showing 1 - 5 of 5
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    Effect of calcium phosphate on growth, pigment and secondary compound accumulation in Cannabis sativa L. callus
    (2026-05-01)
    Tebdoie, C.
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    Thipphaaut, T.
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    Deewatthanawong, R.
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    Kongchinda, P.
    ;
    Singhavorachai, P.
    The application of calcium phosphate in the form of tricalcium phosphate (Ca3(PO4)2) on cannabis (Cannabis sativa L.) callus cultures for 7 days significantly increased fresh and dry weights at concentrations ranging from 800 to 2000 mg/L, with no statistical differences between concentrations. However, higher calcium phosphate concentrations led to reductions in pigments and DPPH radical scavenging activity. By contrast, callus cultures treated with 800 and 1600 mg/L showed enhanced total phenolic content and carotenoids, while total flavonoid content increased proportionally. These findings suggested that calcium phosphate concentration influenced the biosynthesis of secondary metabolites in cannabis callus cultures.
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    Isolation and screening of fish gut actinomycetes for antibacterial activity against Uropathogenic Escherichia coli
    (2023-09-01)
    Jisha, K.
    ;
    Gayathri, G.
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    Gopikrishnan, V.
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    Vareeket, R.
    ;
    Prabha, T. R.
    Actinobacteria are among the most studied prokaryotes due to their propensity to create microbial bioactive compounds. Actinobacterial members can be found in a wide variety of aquatic and terrestrial settings, incorporating marine habitats. Actinomycetes isolated from the marine environment are gaining popularity due to their diverse structures and unique biological properties of the secondary metabolic products. The research was investigated the antibacterial properties of actinobacteria associated with gut of marine fish against human pathogen. Approximately 20 morphologically distinct strains obtained from Engraulis sp. (anchovy fish) and Trachurus trachurus (horse mackerel) were proved for antibacterial efficacy against Extended spectrum beta-lactamase (ESBL) producing uropathogenic Escherichia coli (UPEC). The actinobacterial strain HM7 expressed antibacterial effect against UPEC.
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    Fish gut microbiota: a source of novel metabolites – A review article
    (2023-03-01)
    Jisha, K.
    ;
    Gayathri, G.
    ;
    Gopikrishnan, V.
    ;
    Song, J. J.
    ;
    Soytong, K.
    Similar to human beings, fish harbor microorganisms in their gastrointestinal tract (GIT). Fishes are diverse groups of gut microbiota, including protists, fungi, yeasts, viruses, bacteria, and archaea. In addition to serving as a barrier against infections, these microbes that inhabit the gastrointestinal tract of fish play a role in nutrition, physiology, immunity, and life span. All fish have gut microbiota; however, the makeup of these communities varies depending on the fish's life stages, the habitat they live in, their nutrition, the seasons, their trophic level, etc. For both recreational and commercial fisheries, it is crucial to comprehend the bacterial make-up of fish microbiomes. The gut microbiota aids in the development of methods for modifying the gut microbiota of the target fish species to enhance aquaculture quality. These gut microbes are an invaluable, essential source of novel, promising bioactive compounds with significant biological activity. The natural product secondary metabolites from specific strains of Chaetomium spp. may develop to be biomedicine for sustainable protection. This review provides a comprehensive knowledge of the composition of the gut microbiota of fish, their development, changes in the living environment, their modification, and their applications.
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    Analysis of secondary metabolites and genes related to the pathogenicity of the rice blast fungus Pyricularia oryzae
    (2022-09-01)
    Pradapphai, P.
    ;
    Kumlung, T.
    ;
    Parinthawong, N.
    Pyricularia oryzae causes rice blast disease and is one of the most significant plant pathogens worldwide. The rice blast fungus can produce a variety of phytotoxic secondary metabolites and has been found to be essential for host invasion. Two isolates, virulent isolate RBR55003 and non-virulent isolate CCO56003 were selected which based on their abilities to cause blast symptoms on rice leaves. Pyriculol and picolinic acid were detected in culture filtrate of both isolates during the exponential growth phase. Expression of genes involved in fungal pathogenicity and plant defense were analyzed. The expression of MoPks19 gene in isolate RBR55003 was higher than avirulent isolate CCO56003, indicating that MoPks19 may play a role in the pathogenesis of RBR55003. The expression of the OsWRKY30 gene that plays an important role in the regulation of a defense-related gene in rice was higher in CCO56003 inoculated than in RBR55003 inoculated rice sample, indicating the increased resistance of rice plants occurring in rice after non-virulent isolate infection, while the expression of the OsCPS4 gene involved in phytoalexin synthesis in rice was not different after isolate infections. Gene expression analysis revealed different responses according to plant-pathogen relationships.
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    Functional analysis of a chaetoglobosin A biosynthetic regulator in Chaetomium globosum
    (2021-03-01)
    Cheng, Ming
    ;
    Zhao, Shanshan
    ;
    Liu, He
    ;
    Liu, Yutao
    ;
    Lin, Congyu
    Cytochalasins are a group of fungal secondary metabolites with diverse structures and bioactivities, including chaetoglobosin A production. Chaetoglobosin A is produced by Chaetomium globosum and has potential antifungal activity. Bioinformatics analysis of the chaetoglobosin A gene cluster (che) showed it that consists of nine open reading frames, including those encoding polyketide synthases (PKSs), PKS extender units, post-PKS modifications, and proposed regulators. Here, the role of the CgcheR regulator was investigated using gene disruption experiments. The CgcheR disruptant (ΔCgcheR) completely abolished the production of chaetoglobosin A, which was restored by the introduction of a copy of the wild-type CgcheR gene, suggesting that CgcheR is involved in chaetoglobosin A biosynthesis. A transcriptional analysis of the CgcheR disruptant indicated that CgCheR activates the transcription of chaetoglobosin biosynthetic genes in a pathway-specific manner. Furthermore, constitutive overexpression of CgcheR significantly improved the production of chaetoglobosin A from 52 to 260 mg/L. Surprisingly, CgcheR also played a critical role in sporulation; the CgcheR disruptant lost the ability to produce spores, suggesting that the regulator modulates cellular development. Our results not only shed light on the regulation of chaetoglobosin A biosynthesis, but also indicate a relationship between secondary metabolism and fungal morphogenesis.