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Study on the regeneration of sausage tree (Kigelia africana) by tissue culture
(2026-05-01)
Poeaim, S.
;
Poeaim, A.
This study investigated the regeneration of sausage tree (Kigelia africana) using tissue culture techniques, focusing on callus induction, shoot regeneration from seeds, and root induction from shoot explants. Seeds were cultured on solid MS medium supplemented with various concentrations of BA (0.5, 1, 2, 3, and 5 mg L⁻¹) for 10 weeks. Callus formation was observed at all BA concentrations, with the largest mean callus volume (5214.17 mm³) and highest induction rate (80%) achieved at 5 mg L⁻¹. Shoot regeneration occurred across all BA treatments, with the highest number of shoots (45 shoots; 86.54%) obtained on medium containing 1 mg L⁻¹ BA. Root induction was studied using shoot segments derived from seed-induced shoots cultured on MS medium supplemented with IAA, NAA, or IBA for 4 weeks. IAA at 0.5 mg L⁻¹ induced roots in 33.33% of explants with an average root length of 6.68 mm. NAA at 2 mg L⁻¹ promoted the highest root induction (70.83%), though roots were shorter, while 1 mg L⁻¹ NAA produced fewer roots but with greater length (7.00 mm). IBA at 3 mg L⁻¹ induced rooting in 33.33% of explants with an average root length of 3.78 mm and also stimulated callus formation, with the largest callus volume observed at 2 mg L⁻¹. These results demonstrate that BA is effective for callus and shoot induction, whereas IAA, NAA, and IBA promote root formation, with distinct responses at different concentrations. The optimized protocols provide a foundation for in vitro propagation, mass multiplication, and conservation of K. africana, with potential applications in biotechnology and secondary metabolite production.
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Plant regeneration of Bauhinia purpurea by tissue culture technique
(2024-05-01)
Saparam, W.
;
Poeaim, A.
;
Pongtongkam, P.
;
Chareonsap, P. P.
;
Poeaim, S.
Results showed that the seed of Bauhinia purpurea germination was archived 100% from half-strength MS medium supplemented with 3.0 mg/L Gibberellic acids (GA3) and gave 71.137 mm for the highest seedling. The optimal condition of nodal explants sterilization was E5, which gave 92% of survival explants. For the shoot induction method, the results showed that 24.270 mm was the highest proliferation of shoots achieved from MS medium supplemented with 1.0 mg/L 6-benzylaminopurine (BAP). The highest number of roots was 2.867 roots, and the highest root length was 33.453 mm, obtained at half-strength MS medium complemented with 0.25 mg/L Indole-3-acetic acid (IAA). The 73.33% of plantlets were survived after being transferred to plastic pot.
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Micropropagation of Ironwood (Xylia xylocarpa (Roxb.) Taub.) by tissue culture
(2024-03-01)
Panudom, S.
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Pongtongkam, P.
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Chareonsap, P. P.
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Poeaim, A.
;
Poeaim, S.
It was found that the sterilization procedure was highly effective, resulting in a 100% survival rate of Xylia xylocarpa (Roxb.). After sterilization, the seeds were cultured on a half-strength Murashige and Skoog (MS) medium without adding plant growth regulators (PGRs). A maximum germination percentage of 100% was achieved after 2 weeks of culture. 1.5 cm-sized node segments from both seeds and mother trees were used for shoot induction. These node segments were treated with the same sterilization conditions as the seeds but with the addition of 0.1% HgCl2. The node segments achieved a 90% survival rate despite the additional chemicals. The node segments from seeds were then cultured on a full-strength MS medium supplemented with 0.50 mg/l of BAP. The results showed a maximum number of shoots, reaching 4.10±1.20 per explant and a mean shoot length of 3.87±0.30 cm. The part of node segments derived from mother trees showed 3.50±1.27 shoots per explant, accompanied by a mean shoot length of 3.18±0.89 cm when using BAP concentration of 0.25 mg/l after 12 weeks of cultivation. The rooting medium contained half-strength MS medium supplemented with 0.75 mg/l of IBA. After 8 weeks of being cultured in the rooting medium, the shoots exhibited a maximum rooting percentage of about 80%. The technique provides a valuable method for rapidly propagating and conserving this plant species in a controlled and efficient manner.
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The effect of BA on inducing shoots of Philodendron erubescent 'Pink Princes' in vitro
(2023-11-01)
Chiewchan, N.
;
Saetiew, K.
;
Teerarak, M.
The study investigated the influence of plant growth regulator on the induction of direct shoots from node explants of Philodendron erubescens 'Pink Princes' in vitro condition. The result showed that the most of width, length, and height was observed in MS + BA 0.5 mg/l, measuring 3.40 cm × 3.22 cm × 1.96 cm and 1.74 g fresh weight. The highest number of shoots was consistently observed 60.44 shoots per nodal MDA content (1.91 nmol/g FW) and phenolic content (9.51 mg GAE/g FW) are lowest. The explant was cultured on MS medium performed highest chlorophyll content (chl A 213.96 μg/g FW, chl B 162.54 μg/ FW, total chlorophyll 304.64 μg/FW) and carotenoid content (2.61 μg/g FW) Moreover, they were the largest plant with green leaves when compared to another treatment.
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In vitro propagation from nodal segments of Arachis glabrata cultivar Florigraze
(2020-09-01)
Pholjad, A.
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Pongtongkam, P.
;
Arananant, J.
;
Poeaim, A.
Arachis glabrata Benth. of rhizome perennial peanut is used as a forage plants with higher nutrient value. The nodal segments were used to propagate in vitro by 0.2% of HgCl<inf>2</inf>plus antibiotic and cefotaxime and preservative for plant tissue culture media active (PPM) at 15 min. It was the most effective procedure for disinfecting plant parts (survival of 73.33%). Shoot induction used to the nodal segments of A. glabrata cultivar Florigraze were cultured on MS medium combination with plant growth regulators in cytokinin group at different concentrations 0.5, 0.75, 1, 2, 3 and 5 mg L-1of 6-benzylaminopurine (BAP), N6- furfuryladenine (Kn), meta-Topolin (mT) and Thidiazuron (TDZ). The maximum growth rate was 66.67% on 5 mg L-1of mT was the highest shoot length of 18.43 mm after 30 days. Root induction was achieved on MS medium supplemented with 2 mg L-1of α-Naphthaleneacetic acid (NAA) and 0.2% Activated Charcoal (AC) after 8-12 days cultured when compared with MS medium without plant growth regulators at the basal condition. Thereafter, the plantlets were transferred into the soil for acclimatization. It is the first report using the nodal segment in vitro propagation.

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