KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Search Results

Now showing 1 - 10 of 11

Some of the metrics are blocked by your
consent settings
Silkworm pupae protein isolate as a sustainable functional ingredient: Extraction optimization and application in sponge cake
(2026-01-01)
Yarnpunya, P.
;
Puechkamutr, Y.
;
Pinsirodom, P.
;
Nalinanon, S.
;
Maneerat, N.
The increasing global population necessitates sustainable and nutritious alternative protein sources, positioning edible insects as a promising solution. Silkworm (Bombyx mori) pupae (SP), a by-product of silk production abundant in Thailand, are a rich source of high-quality protein. This study investigated the impact of different extraction solvents (water, saline, and alkaline) on the amino acid profiles, antioxidative capacities, and functional properties (emulsifying and foaming capacities) of SP protein isolates. Alkaline extraction yielded protein isolates (ASP) with a balanced amino acid composition, elevated antioxidant activities, and superior emulsifying activity index and foaming stability, compared to bovine serum albumin. Consequently, ASP was selected to enrich sponge cake, a low-ratio foam-type cake that relies on air bubble stability. Sponge cakes with 0, 5, 10 and 15% wheat flour substitution by ASP were prepared and evaluated for their quality and sensory properties. While water activity remained unaffected, the specific volume of SP sponge cakes significantly increased with higher ASP substitution levels. Texture analysis revealed a softening effect at lower substitution rates (up to 10%), followed by increased hardness at 15%. Sensory evaluation indicated no significant difference in liking scores in appearance, colour, and texture across all formulations, but liking scores in odour and taste declined with increasing ASP concentration. Overall, the study demonstrates the potential of alkaline-extracted SP protein isolates as a functional ingredient in sponge cake up to a 5% substitution level, improving specific volume without compromising sensory acceptability. Further research is needed to address off-flavours and explore broader applications in bakery products.
Some of the metrics are blocked by your
consent settings
Exploring Tectona grandis Linn. f. Leaf Extract as a Functional Feed Additive with Antioxidant and Nutraceutical Potential for Livestock
(2025-12-01)
Montri, Nattaya
;
Wanapat, Metha
;
Kang, Sungchhang
;
Cheas, Seangla
;
Cherdthong, Anusorn
This study investigated the phytochemical composition, antioxidant activity, and safety of Tectona grandis leaf extracts at four maturity stages. Ethanolic extracts were screened for secondary metabolites and analyzed using GC–MS, together with heavy metal determination, phenolic profiling, and antioxidant assays. Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, glycosides, tannins, saponins, and coumarins, with clear variation among leaf stages. GC–MS analysis detected multiple bioactive constituents, including phytol, n-decanoic acid, and heptadecenal. These compounds have been previously reported to exhibit antioxidant, antimicrobial, and anti-inflammatory properties. Heavy metal analysis showed very low cadmium concentrations (0.001–0.004 mg/kg), undetectable lead levels (<LOD), and trace levels of arsenic concentrations (0.012–0.018 mg/kg), all of which were within safe limits for plant materials. Quantitative assays demonstrated that mature leaves contained the highest total phenolic (8.751 ± 0.018 mg GAE/g DW), total flavonoid (0.359 ± 0.017 mg QE/g DW), and condensed tannin (0.303 ± 0.000 mg CE/g DW) contents. Correspondingly, mature-leaf extracts exhibited the greatest antioxidant capacity, showing 95.88 ± 0.12% inhibition of oxidant activity. The ethanolic extracts exhibited IC<inf>50</inf> values ranging from 22.93–50.35 mg/mL, whereas the ascorbic acid standard showed an IC<inf>50</inf> of 0.0767 mg/mL, confirming the expected lower potency of crude plant extracts. The strong correlation between phenolic accumulation and antioxidant activity suggests enhanced defense against oxidative stress. The absence of detectable heavy metal contamination, together with the high polyphenolic content, suggests that T. grandis leaves may serve as a safe natural source of antioxidants. These findings demonstrate the strong antioxidant potential of T. grandis leaf extracts, providing a basis for future studies evaluating their functional applications in biological systems.
Some of the metrics are blocked by your
consent settings
Nutritional value, antioxidant activity and color properties of white-fleshed sweet potatoes in Vietnam
(2025-01-01)
Dung, Nguyen Chi
;
Giau, Tran Ngoc
;
Van Hao, Hong
;
Minh, Vo Quang
;
Van Tai, Ngo
Sweet potatoes have been considered an essential food source in the human diet for centuries. The study provides comprehensive knowledge on the physico-chemical properties and antioxidant potential of white-fleshed sweet potato (WFSP), to raise consumer awareness of its positive health effects and encourage its greater use. The study also explores whether the food is acidic or alkaline to enhance its health benefits, through Potential Renal Acid Load (PRAL) score. Phenolic acid components were identified and quantified by Ultra-Performance Liquid Chromatography (UPLC), while the functional groups of these compounds were determined by Fourier transform infrared (FTIR) spectroscopy. The results showed that WFSP contains high levels of minerals, including potassium 79.56 mg/100 g, phosphorus 18.69 mg/100 g, magnesium 9.58 mg/100 g and calcium 7.68 mg/100 g. Resistant starch is also present in WFSP at a relative amount of 1.12 %. The total polyphenol content and total flavonoid content were found to be 1.58 mg GAE/g and 0.69 mg QE/g, respectively and it was a source of antioxidant potential (with IC<inf>50</inf> DPPH and IC<inf>50</inf> ABTS·+ determined as 3.31 mg/mL and 0.38 mg/mL, respectively). WFSP was identified as an alkaline food with a negative value (-0.63). Low-PRAL/alkali-ash diets have been linked to a variety of health benefits, including an improved potassium-to-sodium ratio, less muscle wasting and higher intracellular magnesium concentrations. FTIR analysis showed that the phenolic components in WFSP contained 17 functional groups. UPLC analysis identified the main phenolic acids in WFSP as caffeic acid, coumaric acid and trans-ferulic acid, which are antioxidant compounds that can protect cells from damage.
Some of the metrics are blocked by your
consent settings
Enzymatic hydrolysis and biological activities of Konjac glucomannan hydrolysate in different degree of polymerisation
(2024-11-01)
Pomsang, Pachara
;
Ayuni, Dwi
;
Phumsombat, Putthapong
;
Eugelio, Fabiola
;
Fanti, Federico
The study explores the enhanced functional and bioactive properties of Konjac Glucomannan Hydrolysate (KGMH) by partially degrading Konjac Glucomannan (KGM) using β-mannanase over 60 min. KGM concentrations (40% and 50% w/w) were treated with 200–300 U g−1 of the enzyme over 60 min. The hydrolysis of KGM was monitored by colorimetry, with DP values ranging from 4.20 to 6.16 for 40% KGM and 4.10 to 4.60 for 50% KGM. MALDI-TOF-MS analysis confirmed typical oligosaccharides with DP values from 2 to 9 and some acetyl substitutions. The optimal conditions of 40% KGM with 250 U g−1 enzyme and varying hydrolysis times produced KGMHs with a wide range of DPs, demonstrating in vitro antioxidant and anti-glycation activities. The results showed significant bioactivities (P < 0.05) positively correlated with lower DP values. This study emphasises the potential of KGMH as a novel functional food ingredient, highlighting its bioactive properties and the significant impact of DPs on the biological functionality of saccharides.
Some of the metrics are blocked by your
consent settings
Postharvest UV-C Treatment Affects Bioactive Compounds and Maintains Quality of Okra (Abelmoschus esculentus L.) during Storage
(2024-01-01)
Techavuthiporn, Chairat
;
Jarerat, Amnat
;
Singhkai, Chonlada
;
Nimitkeatkai, Hataitip
The effects of UV-C treatment on physicochemical quality, bioactive compounds and antioxidant activity of okra (Abelmoschus esculentus L.) during storage were investigated. Okra pods were exposed to UV-C irradiation dose at 1.5, 3.0 or 6.0 kJ·m−2 before storage at 10 ± 2°C for 12 days. The results showed that UV-C treatment had a significant effect on okra bioactive compounds, such as ascorbic acid, total phenolic, flavonoid contents and antioxidant activity. The highest values of these compounds were observed in okra treated with 6.0 kJ·m−2 UV-C irradiation. A statistical analysis of the data demonstrated that mucilage had a favorable association with antioxidant capabilities and bioactive substances in response to UV-C treatment. Moreover, UV-C treatment also effectively maintained the quality of okra during storage, as evidenced by lower weight loss and higher firmness without incidence of decay. Therefore, postharvest UV-C treatment can be a potential approach to enhance bioactive compounds and maintain the quality of okra during storage.
Some of the metrics are blocked by your
consent settings
Effects of steaming and drying on quality and antioxidant activity of white-fleshed sweet potato powder (Ipomoea batatas)
(2024-01-01)
Thuy, N. M.
;
Giau, T. N.
;
Hao, H. V.
;
Dung, N. C.
;
Tai, N. V.
White-fleshed sweet potatoes (WFSP) are rich sources of polyphenols. The flesh of PFSP contains total polyphenol content (TPC) at 1.52 mgGAE/g fresh weight. The raw material’s total free radical scavenging capacity was estimated (at 42.4% DPPH). The inactivation of peroxidase (POD) and polyphenol oxidase (PPO), the breakdown of total phenolic compounds, antioxidant activity, and the whiteness of WFSP were evaluated during steam blanching from 0 to 9 minutes. Steam blanching time from 7 to 9 minutes at boiling temperature (100ºC) inactivated most of the POD and PPO enzymes (71.2% and 70.5%, respectively), thus TPC and antioxidant activity remains high, resulting in a bright white product. After 7 minutes of blanching, drying experiments of WFSP slices were conducted at air temperatures of 50 to 80ºC. The best alternative for describing and understanding the drying of WFSP slices was the Page model. In the temperature’s ranges, the values of the moisture diffusivity (De f f) and activation energy are obtained from 3.059×10−12 to 5.095×10−12 m2 /s and 14.93 kJ/mol, respectively. The drying temperature of 70ºC for 10 hours was suitable for producing WFSP powder with low water activity (0.5) and moisture content (6.5%), high TPC and antioxidant activity, and low specific energy consumption. These results suggested that the WFSP powder exhibited potential as a nutraceutical ingredient product.
Some of the metrics are blocked by your
consent settings
Chemical Analysis and Bioactivities of Gaultheria procumbens L. Essential Oil: Herbicidal, Antioxidant and Antibacterial Activities
(2023-03-02)
Jintanasirinurak, Sumonrat
;
Manichart, Nutcha
;
Somala, Naphat
;
Laosinwattana, Chamroon
;
Teerarak, Montinee
The chemical composition of wintergreen (Gaultheria procumbens L.) essential oil (WEO) was identified by gas chromatography-mass spectrometry (GC-MS) and evaluated for its biological activities. Methyl salicylate (100%) was the main compound in the wintergreen essential oil. The herbicidal efficacy of the WEO was confirmed by germination inhibition, radical and shoot length reduction, and phytotoxicity assessment with Echinochloa crus–galli and Amaranthus tricolor seedlings. The WEO significantly reduced the germination and seedling growth of both weeds. It showed highly significant inhibition of seed germination (93.37%) and seedling growth of A. tricolor at the highest dose (6 µL/petri dish) assayed. The WEO was formulated as an emulsifiable concentrate (EC-EO) for post-emergence application, and was applied at a range of concentrations from 10 to 80 mL/L. Both treated plant leaves appeared wilted and slightly discolored within 1 day of application (DAA). The visible weed control efficacy was most remarkable at 80 mL/L treatment, which was a level that ultimately killed the seedlings of both species at 3 DAA, suggesting promising herbicidal potential for the WEO. The WEO showed weak scavenging activity with a high IC<inf>50</inf> value (IC<inf>50</inf> >2×104 and >5×104 ppm) for DPPH scavenging and metal chelating assays, respectively. The WEO showed moderate antibacterial activity, and its zones of inhibition against bacterial test strains were 7.90±0.8 mm and 23.9±0.9 mm for Escherichia coli TISTR 780 and Staphylococcus aureus TISTR 1466, respectively. Therefore, the results suggest the possibility of using WEO as an active ingredient to produce natural herbicides.
Some of the metrics are blocked by your
consent settings
Short-term Anoxic Treatment of Litchi Fruit (Litchi chinensis Sonn. cv. Hong Huey) Delays Pericarp Browning and Maintains Physicochemical Quality During Storage
(2023-01-01)
Techavuthiporn, Chairat
;
Jarerat, Amnat
;
Nimitkeatkai, Hataitip
The effect of short-term anoxic treatment prior to storage at ambient and cool temperatures on pericarp browning, fruit quality, secondary metabolites, antioxidant activity, and the browning enzyme of litchi (Litchi chinensis Sonn.) cv. Hong Huey were investigated. Litchi fruit were exposed to anoxic conditions for 6, 12, 18 and 24 h before storage at 28 ± 2°C for 5 days, or at 7 ± 2°C for 14 days. Anoxic treatment resulted in significantly decreases in electrical conductivity, weight loss, browning index, while maintaining the total soluble solids (TSS) and delaying increases in polyphenol oxidase (PPO) and peroxidase (POD) activities compared with control fruit. Furthermore, anoxic treatment increased litchi pericarp methanol extract antioxidant capacity, as measured by free-radical scavenging activity. This is associated with greater amounts of ascorbic acid, anthocyanins, and phenolic/flavonoid components as compared with control fruit. Additionally, anoxic treatment considerably delayed litchi fruit pericarp browning. This suggests that with adequate short-term anoxia duration, an enhanced non-enzymatic antioxidant process may directly or indirectly delay litchi pericarp browning. Thus, a short anoxic treatment enables harvested litchi quality to be sustained at ambient and cool temperatures. This non-chemical and inexpensive treatment deserves further development and application, especially in commercial distribution systems where cooling is insufficient.
Some of the metrics are blocked by your
consent settings
EVALUATION OF BIOACTIVITIES OF EICHHORNIA CRASSIPES PARTS EXTRACTS
(2023-01-01)
Sriwatcharakul, S.
The aims of this research to study bioactivity of leaf, stolon and root extracts from Eichhornia crassipes that is an alien plant in Thailand. The determination of phytochemical exhibited that! leaf extract had the highest of flavonoid and tannin as 207.60 mg QE/g extract and 72.40 mg TAE/g extract, respectively. And root extract showed the highest level of anthocyanin as 17.53 mg cyanidin-3-glucoside/ l extract, while total phenolic contents not significant between leaf, root and stolon extract. To analyze antioxidant activity found that stolon extract had the highest scavenging activity that IC50 was 3.23 mg/ml. The antibacterial activity exhibited that stolon extract had the lowest minimum bactericidal concentration than other two extracts against Pseudomonas aeruginosa TISTR 781, Escherichia coli TISTR 780, Staphylococcus aureus TISTR 1466 and Yersinia enterocolitica. According to the phytochemicals and bioactivities results, stolon and leaf extracts of E. crassipes have potential for remedy and health care aspects to add value of this plant.
Some of the metrics are blocked by your
consent settings
BIOACTIVITIES EVALUATION OF THE USELESS PARTS OF ACACIA AURICULIFORMIS EXTRACTS
(2022-01-01)
Sriwatcharakul, S.
This research aims to study bioactivity of leaf, inflorescence and branch extracts from Acacia auriculiformis that has been used as forest plantation in Thailand. The extracts were evaluated of antioxidant activities by DPPH scavenging method, branch extract gave the best 50 percent Inhibition Concentration (IC50) at 19.36 mg/ml. To find out total phenolic contents, branch extract had the highest total phenolic contents at 597.22 mg GAE/g extract. Antibacterial studied of extracts against five bacterial strains; Escherichia coli TISTR 780, Staphylococcus aureus TISTR 1466, Micrococcus luteus TISTR 2374, Bacillus cereus TISTR 687 and Xanthomonas campestrispv.campestris TISTR 2065. The result of antibacterial activity using agar well diffusion method was found that all of extracts could not inhibit the growth ofE. coliwhileleaf, inflorescence and branch extract could inhibit the growth ofother four bacterial strains S. aureus, M. luteus B. cereus and X. campestrispv campestris. And MIC studied found thatinflorescence extract used the lowest concentration to inhibit the growth ofS. aureus, M. luteus, B. cereus and X. campestrispv campestris. According to the bioactivities results, branch and inflorescence extracts of A. auriculiformis have potential for remedy aspects to add value of this plant.

KMITL

Browse

Filters

Settings

Sort By

Results per page

Search Results