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Start date: 2025Subject: search.filters.subject.Green chemistry

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    A scanner-based microplate platform for high-throughput oxytetracycline detection using diazotization-coupling chemistry with greenness evaluation
    (2026-06-01)
    Puttasa, Papawarin
    ;
    Ngernpimai, Sawinee
    ;
    Tippayawat, Patcharaporn
    ;
    Park, Myoung‑Hwan
    ;
    Teerasong, Saowapak
    Routine oxytetracycline (OTC) monitoring of aquaculture effluents, water sources, and pharmaceutical products is essential for environmental management and regulatory compliance. However, existing analytical methods remain largely instrumental and low-throughput. Herein, a microvolume, high-throughput, scanner-based colorimetric microplate platform is developed for OTC determination using a sensitive and selective diazotization-coupling chemistry with RGB digital image analysis. Sulfanilamide is diazotized with nitrite to generate reactive diazonium species, which subsequently couple with OTC to form a strongly colored yellow-orange azo dye. Only microliter-scale volumes of reagents and samples are required in each well, and the reaction is completed within a few minutes, enabling simultaneous analysis of up to 96 samples. The microplate is imaged with a flatbed scanner, and color responses are quantified using ImageJ software. A linear calibration range of 1 − 40 μM was obtained with a detection limit of 0.33 μM. The platform was successfully applied to OTC determination in aquaculture effluents, tap water, drinking water, and pharmaceutical samples. It exhibited high selectivity against other antibiotics with no observable cross-reactivity. The green analytical procedure index (GAPI) confirmed the method’s environmental friendliness. Overall, the scanner-based microplate platform offers a simple, low-cost, portable, and high-throughput alternative to conventional OTC assays.
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    Insights from dual-platform metabolomics on durian flowers: An alternative source of procyanidins from agricultural waste with bioactivities
    (2025-07-01)
    Potijun, Supakorn
    ;
    Pattarapipatkul, Nattaya
    ;
    Boonma, Pitchakorn
    ;
    Pewlong, Putthamas
    ;
    Pathtubtim, Intira
    Procyanidins, which are polyphenol compounds in grape seeds, apples, and berries, are known for their anti-inflammatory and antioxidant properties. In this study, we investigated durian flowers, an agricultural waste, as a novel procyanidin source. Durian trees bloom prolifically, but not all flowers develop into mature fruits, representing underutilized resources. Dual-platform metabolomic analysis using ultra-high-performance liquid chromatography with electrospray ionization quadrupole time-of-flight mass spectrometry and gas chromatography–mass spectrometry annotates polyphenols such as (−)-epicatechin, procyanidins B1, B2, and C1. The 80 % (v/v) ethanol extraction yielded a crude extract with a total procyanidin content of 7.68 mg/g. Bioactivity assays revealed that the procyanidin-rich crude extract reduced oxidative stress and exhibited anti-inflammatory effects against UVA in human keratinocytes (HaCaT). This study is the first to propose durian flowers as a sustainable and cost-effective procyanidin source with potential application in the nutraceutical and cosmeceutical industries, contributing significantly to the repurposing of agricultural waste through green technology.