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    Comparative genomics reveals insight into the phylogeny and habitat adaptation of novel Amycolatopsis species, an endophytic actinomycete associated with scab lesions on potato tubers
    (2024-01-01)
    Wannawong, Thippawan
    ;
    Mhuantong, Wuttichai
    ;
    Macharoen, Pipat
    ;
    Niemhom, Nantawan
    ;
    Sitdhipol, Jaruwan
    A novel endophytic actinomycete, strain MEP2-6<sup>T</sup>, was isolated from scab tissues of potato tubers collected from Mae Fag Mai Sub-district, San Sai District, Chiang Mai Province, Thailand. Strain MEP2-6<sup>T</sup> is a gram-positive filamentous bacteria characterized by meso-diaminopimelic acid in cell wall peptidoglycan and arabinose, galactose, glucose, and ribose in whole-cell hydrolysates. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and hydroxy-phosphatidylethanolamine were the major phospholipids, of which MK-9(H<inf>6</inf>) was the predominant menaquinone, whereas iso-C<inf>16:0</inf> and iso-C<inf>15:0</inf> were the major cellular fatty acids. The genome of the strain was 10,277,369 bp in size with a G + C content of 71.7%. The 16S rRNA gene phylogenetic and core phylogenomic analyses revealed that strain MEP2-6<sup>T</sup> was closely related to Amycolatopsis lexingtonensis NRRL B-24131<sup>T</sup> (99.4%), A. pretoriensis DSM 44654<sup>T</sup> (99.3%), and A. eburnea GLM-1<sup>T</sup> (98.9%). Notably, strain MEP2-6<sup>T</sup> displayed 91.7%, 91.8%, and 87% ANIb and 49%, 48.8%, and 35.4% dDDH to A. lexingtonensis DSM 44653<sup>T</sup> (=NRRL B-24131<sup>T</sup>), A. eburnea GLM-1<sup>T</sup>, and A. pretoriensis DSM 44654<sup>T</sup>, respectively. Based on phenotypic, chemotaxonomic, and genomic data, strain MEP2-6<sup>T</sup> could be officially assigned to a novel species within the genus Amycolatopsis, for which the name Amycolatopsis solani sp. nov. has been proposed. The type of strain is MEP2-6<sup>T</sup> (=JCM 36309<sup>T</sup> = TBRC 17632<sup>T</sup> = NBRC 116395<sup>T</sup>). Amycolatopsis solani MEP2-6<sup>T</sup> was strongly proven to be a non-phytopathogen of potato scab disease because stunting of seedlings and necrotic lesions on potato tuber slices were not observed, and there were no core biosynthetic genes associated with the BGCs of phytotoxin-inducing scab lesions. Furthermore, comparative genomics can provide a better understanding of the genetic mechanisms that enable A. solani MEP2-6<sup>T</sup> to adapt to the plant endosphere. Importantly, the strain smBGCs accommodated 33 smBGCs encoded for several bioactive compounds, which could be beneficially applied in the fields of agriculture and medicine. Consequently, strain MEP2-6<sup>T</sup> is a promising candidate as a novel biocontrol agent and antibiotic producer.
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    Amycolatopsis rhizosphaerae sp. Nov., isolated from rice rhizosphere soil
    (2018-05-01)
    Thawai, Chitti
    A novel actinomycete, designated strain DH51B-4-3<sup>T</sup>, which formed long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium, was isolated from rice rhizosphere soil. The isolate contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars of strain DH51B-4-3<sup>T</sup> were arabinose, galactose, glucose, rhamnose and ribose. The phospholipids in the cell membrane were phosphatidylethanolamine, phosphatidyl methylethanolamine, diphosphatidylglycerol and phosphatidylinositol. The major menaquinone was MK-9(H<inf>4</inf>). The main cellular fatty acids were iso-C<inf>16:0</inf> and cyclo-C<inf>17:0</inf>. The G+Ccontent of the genomic DNA was 68.2mol%. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3<sup>T</sup> should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904<sup>T</sup> (98.06%) and Amycolatopsis sacchari DSM 44468<sup>T</sup> (97.77%). The result of DNA-DNA hybridization and some physiological and biochemical properties indicated that strain DH51B-4-3<sup>T</sup> could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Amycolatopsis rhizosphaerae sp. nov. is proposed. The type strain is DH51B-4-3<sup>T</sup> (=TBRC 6029<sup>T</sup>=NBRC 112509<sup>T</sup>).