KMITL
Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/kmitl/1
Browse
3 results
Search Results
- Some of the metrics are blocked by yourconsent settings
Item type:Publication, Preharvest blue diode laser irradiation enhances betalain biosynthesis, antioxidant responses, and postharvest quality of red amaranth microgreens during storage(2026-12-01); ;Sripumimas, Sookmas ;Li, Man ;Kang, ChuankaiPuisoongnoen, NichaphatIn recent years, light-based technologies have attracted growing interest as a postharvest strategy for maintaining the quality of leafy vegetables and microgreens. This study investigated the effects of blue diode laser irradiation (450 nm, 3 h) on the postharvest quality, antioxidant system, and betalain biosynthesis of red amaranth (Amaranthus tricolor) microgreens during storage at 4 ± 1 °C for 9 d. Blue laser treatment effectively maintained visual quality and reduced weight loss compared with untreated samples. Treated microgreens exhibited significantly lower accumulation of hydrogen peroxide (H₂O₂), superoxide anion (O₂⁻), and lipoxygenase (LOX) activity during storage, indicating reduced oxidative stress and membrane lipid peroxidation. In addition, laser-treated samples maintained higher antioxidant capacity, including ferric reducing antioxidant power (FRAP) and DPPH radical scavenging activity, together with greater retention of total phenolics, flavonoids, vitamin C, and glutathione. Activities of antioxidant-related enzymes, including phenylalanine ammonia-lyase (PAL), superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), were also significantly enhanced by laser irradiation. Furthermore, blue laser treatment effectively delayed chlorophyll degradation and increased betacyanin accumulation during storage. Gene expression analysis revealed significant upregulation of betalain biosynthesis-related genes, including AtrWRKY42–2, AtrCYP76AD1, AtrMYB72, AtrDODA1–1, and AtrB5-GT, particularly during the early storage period. Principal component analysis clearly separated laser-treated and control samples, confirming the positive association between laser irradiation, antioxidant defense, pigment retention, and overall quality maintenance. These findings demonstrate that short-term blue laser irradiation is a promising postharvest technology for enhancing antioxidant defense, regulating betalain biosynthesis, and extending the shelf life of red amaranth microgreens.1 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Translational Value of Natural Pigments with Reference to Healthcare and Cosmetic Applications(2024-01-01) ;Saravanan, Tamil Selvam ;Gopikrishnan, Venugopal ;Manikkam, Radhakrishnan ;Kaari, ManigundanAnnamalai, Kishore KumarThe natural pigments have reported to be using in a wide range of industries, agriculture, textiles, cosmetics, pharmaceuticals, and food. They have become a necessary component of our daily safety life. Synthetic dyes can be harmful impacts to human being as consumer and worker’s health as well as negatively affect to the environment. Numerous natural pigments are recognised as intriguing bioactive substances with feasible safety health advantages. Plants and microbes are discovered to be the main suppliers as natural pigments which various application for these natural products. Recently, the use of natural pigments in the food industry has increased in many areas, including pharmacology, toxicology, textile, and printing industries, as well as food, dairy, and fish industries. The amount of food waste produced has significantly increased as a result of the current surge in industrial food production, particularly the leftovers of fruits and vegetables which are a good source of natural colours including anthocyanins, betalains, carotenoids, and chlorophyll which have both decorative and medicinal effects. Therefore, it is crucial for both economic and environmental reasons to recover the natural pigments from food waste. The cost-effective production would be appreciated and investigated would be overshadowed by the advantages for people. The research findings must be investigated to develop the readily available, non-toxic, environmentally responsible, affordable, and biodegradable pigments for multipurpose application and especially for food security and safety.1 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Identification of major carotenoids from green alga Tetraspora sp. CU2551: partial purification and characterization of lutein, canthaxanthin, neochrome, and β-carotene(2022-08-01) ;Maswanna, ThanapornThe green algae Tetraspora sp. CU2551 was previously identified as a strain with high potential for biohydrogen production; however, its algal biomass characteristics changed from green to reddish orange within 43 days of biohydrogen production. The crude pigments were extracted, partially purified, and characterized by chemical determination. The present study focused on elucidating the carotenoid composition of the selected green alga Tetraspora sp. CU2551. The pigment extract was partially purified and fractionated using thin layer chromatography, and yielded two major and two minor carotenoid bands. The fractions were confirmed by high-performance liquid chromatography with a diode array detector (HPLC–DAD) before being identified and confirmed using Liquid Chromatograph-Quadrupole Time of Flight-Mass Spectrometry (LC-QTOF-MS). The spectral data of these fractions revealed four sub-fractions of interest that were lutein, canthaxanthin, neochrome, and β-carotene, which had percentages in the crude extracts of 30.57%, 25.47%, 7.89%, and 0.71%, respectively. Lutein and canthaxanthin were found to be the major carotenoid pigments present. Our findings in this present study are the first reporting of Tetraspora sp. CU2551 as a potential alternate source for carotenoid pigment production.1
