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Subject: search.filters.subject.16s rrna gene

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    Thermocatellispora soli sp. nov., isolated from hot spring soil
    (2019-01-01)
    Duangupama, Thitikorn
    ;
    Pittayakhajonwut, Pattama
    ;
    Thawai, Chitti
    An aerobic, spore-forming, actinomycete, designated strain CHM3-46 <sup>T</sup> , was isolated from soil in a hot spring pond located in Chiangmai province, Thailand. The strain exhibited taxonomic characteristics consistent with the genus Thermocatellispora. Strain CHM3-46 <sup>T</sup> produced short, straight chains of warty spores on aerial mycelia. The presence of meso-diaminopimelic acid was observed in the cell-wall peptidoglycan. The whole-cell reducing sugars were glucose, mannose, galacose and ribose. The phospholipids comprised phosphatidylmethylethanolamine, hydroxyphosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, four phosphoglycolipids and three unidentified phospholipids. The predominant menaquinones were MK-9(H <inf>4</inf> ), MK-9(H <inf>6</inf> ) and MK-9 (H <inf>8</inf> ). 10-methyl C <inf>17 : 0</inf> , C <inf>16 : 0</inf> , C <inf>17 : 0</inf> and iso-C <inf>16 : 0</inf> were identified as the main cellular fatty acids. The G+C content of the genomic DNA was 73.2 mol%. Analysis of the 16S rRNA gene sequence showed that strain CHM3-46T belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521 <sup>T</sup> (98.5 %). Furthermore, a low DNA relatedness value (23.4 %±0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46 <sup>T</sup> and its closest relative. Based on the taxonomic data, strain CHM3-46 <sup>T</sup> could be readily distinguished from its closest phylogenetic relative and represents a novel species, for which the name Thermocatellispora soli sp. nov. is proposed. The type strain is CHM3-46 <sup>T</sup> (=TBRC 7649 <sup>T</sup> =NBRC 113148 <sup>T</sup> ).
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    Microbispora soli sp. nov., isolated from soil of a hot spring
    (2018-12-01)
    Kittisrisopit, Suchada
    ;
    Pittayakhajonwut, Pattama
    ;
    Tadtong, Sarin
    ;
    Thawai, Chitti
    A novel actinomycete, strain RTBAU4-9 <sup>T</sup> , which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area. This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H <inf>4</inf> ), MK-9(H <inf>2</inf> ) and MK-9(H <inf>0</inf> ) as the characteristic menaquinones, and iso-C <inf>16: 0</inf> and anteiso-C <inf>17: 0</inf> as the main fatty acids. The G+C content of the genomic DNA was 70.2 mol%. The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020 <sup>T</sup> (98.3% 16S rRNA gene sequence similarity). In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9 <sup>T</sup> should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp. nov. is proposed. The type strain is RTBAU4-9 <sup>T</sup> (=TBRC 7648 <sup>T</sup> =NBRC 113147 <sup>T</sup> ).
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    Jishengella zingiberis sp. Nov., isolated from root tissue of zingiber montanum
    (2018-10-01)
    Thawai, Chitti
    ;
    He, Ya Wen
    ;
    Tadtong, Sarin
    A novel actinomycete, strain PLAI 1-1<sup>T</sup>, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum. The isolate contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid in the cell-wall peptidoglycan. The acyl type of the cell-wall muramic acid was glycolyl. The whole-cell sugars of strain PLAI 1-1<sup>T</sup> were glucose, arabinose, xylose, ribose and a trace amount of mannose. The membrane phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol. The major menaquinone was MK-9 (H<inf>4</inf>). The main cellular fatty acids were iso-C<inf>15: 0</inf> and C<inf>17: 1</inf> w8c. The G+C content of the genomic DNA was 70.6 mol%. 16S rRNA gene sequence analysis revealed that strain PLAI 1-1<sup>T</sup> was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430<sup>T</sup> (99.2 %). Based on the data of physiological and biochemical tests, including the result of DNA–DNA hybridization, strain PLAI 1-1<sup>T</sup> represents a novel species of the genus Jishengella, for which the name Jishengella zingiberis sp. nov. is proposed. The type strain is PLAI 1-1<sup>T</sup> (=TBRC 7644<sup>T</sup> =NBRC 113144<sup>T</sup> ).