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Subject: search.filters.subject.Acetone

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    Item type:Publication,
    Impact of Dimethylformamide/Acetone Ratios and Stirring Time on Morphology and Electrical Properties of Electrospun PVDF Fibers
    (2025-01-01)
    Hakimi Bin Ismail, M. I.
    ;
    Syed Bakar, S. S.
    ;
    Yahud, S.
    ;
    Chaisitsak, S.
    This study examines the impact of Dimethylformamide, DMF/Acetone ratio (60/40, 70/30 and 100/0) and stirring time (16 hours and 24 hours) on Polyvinylidene Fluoride electrospun fibers. Total of six samples were prepared at 12% solution con-centration were electrospun at 20 kV, 10 cm tip-to-collector distance, and 1 mL/h flow rate. Morphological analysis showed that a 70/30 ratio produced finer fibers with fewer beads than 60/40 ratio, while prolonged stirring increased fiber diameter. Electrical analysis confirmed the lowest resistance and highest conductivity for these fibers. The 70/30 ratio and 16-hour stirring time technique optimized fiber structure and electrical performance for advanced applications.
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    Partial purification and comparison of precipitation techniques of pyruvate decarboxylase enzyme
    (2017-01-01)
    Tangtua, Julaluk
    ;
    Techapun, Charin
    ;
    Pratanaphon, Ronachai
    ;
    Kuntiya, Ampin
    ;
    Sanguanchaipaiwong, Vorapat
    The intracellular pyruvate decarboxylase enzyme (PDC, EC 4.1.1.1) extract from Candida tropicalis TISTR 5350 was compared by two different purification methods using ammonium sulphate and acetone precipitation. The total volumetric PDC activity and percentage recovery (yield) of precipitated PDC based on 50% (v/v) cold acetone were significantly higher (1.13 ± 0.02 U/ml and 98.27 ± 2.98 %, respectively) than any other concentration levels of acetone used. Furthermore, all concentration levels of cold acetone also yielded a much higher specific PDC activity than the precipitate obtained using the 40 to 60% (w/v) ammonium sulphate saturation (0.75 ± 0.08 U/mg protein). The precipitated enzyme in buffer solutions from the 50% (v/v) acetone was subsequently freeze dried. Freeze drying of the precipitated PDC by cold acetone resulted in the specific PDC activity of 1.57 ± 0.02 U/mg protein and differed statistically (p ≤ 0.05) from the crude enzyme extract (control).