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    Item type:Publication,
    Application of soil test kit for evaluating nitrogen fertilizer requirement of Napier Pakchong 1 grass in Thailand
    (2018-12-01) ;
    A soil test kit may be applicable for nitrogen management in pasture grass production. The nitrogen fertilizer requirement of Napier Pakchong 1 grass (Pennisetum purpurem ×Pennisetum amenicanum cv. Pakchong 1) was evaluated by using a soil test kit (STK). Result showed that the application of nitrogen fertilizer in the forms of urea and ammonium sulphate increased in dry mater yield of Napier Pakchong 1 grass, whereas application of cattle manure at both recommended rates and the recommended rate by STK gave the dry matter yield similar to the unfertilized control. Application of ammonium sulphate gave better than application of urea. The clay soil with moderate soil fertility and high soil moisture content, the soil test kit provided the acceptable guideline for nitrogen management in Napier Pakchong 1 grass. The application of the soil test kit for nitrogen fertilizer management of Napier Pakchong 1 grass in other soil types are noted.
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    Item type:Publication,
    Partial purification and comparison of precipitation techniques of pyruvate decarboxylase enzyme
    (2017-01-01)
    Tangtua, Julaluk
    ;
    Techapun, Charin
    ;
    Pratanaphon, Ronachai
    ;
    Kuntiya, Ampin
    ;
    The intracellular pyruvate decarboxylase enzyme (PDC, EC 4.1.1.1) extract from Candida tropicalis TISTR 5350 was compared by two different purification methods using ammonium sulphate and acetone precipitation. The total volumetric PDC activity and percentage recovery (yield) of precipitated PDC based on 50% (v/v) cold acetone were significantly higher (1.13 ± 0.02 U/ml and 98.27 ± 2.98 %, respectively) than any other concentration levels of acetone used. Furthermore, all concentration levels of cold acetone also yielded a much higher specific PDC activity than the precipitate obtained using the 40 to 60% (w/v) ammonium sulphate saturation (0.75 ± 0.08 U/mg protein). The precipitated enzyme in buffer solutions from the 50% (v/v) acetone was subsequently freeze dried. Freeze drying of the precipitated PDC by cold acetone resulted in the specific PDC activity of 1.57 ± 0.02 U/mg protein and differed statistically (p ≤ 0.05) from the crude enzyme extract (control).
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