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    Item type:Publication,
    The Study of Image Quality Effect on Model Performance for Bacteria Classification
    (2025-01-31)
    Treebupachatsakul, Treesukon
    ;
    Chomkwah, Wanwalee
    ;
    Tanpatanan, Tananan
    ;
    Poomrittigul, Suvit
    One of the key requirements for supervised learning in deep learning model construction is the dataset for training and validation. For gathering the dataset, obtaining various image qualities from different resources is unavoidable, and this has been considered to affect the supervised model performance. This research proposes to demonstrate the effect of image quality involving high and standard datasets obtained from 2 different resources on the performance of models. The various cell characteristics with gram-positive and gram-negative bacteria datasets were challenged for trial. These different datasets were matched and contributed to 5 cases; case 1: train and test with high-quality images, case 2: train with high-quality images and test with standard quality images, case 3: train and test with images of standard quality, case 4: train with standard-quality images and test with high-quality images, and case 5: train and test with combining these two image qualities. Pre-trained CNN models were implemented to prove the purpose with and without stratified K-fold cross-validation. The results of retrained models showed that the high-performance models require high-quality datasets obtained from the same resource as the testing set, which yield more than 90% of all performance evaluation metrics when tested on challenging unseen datasets. This study provides valuable insights for building high-performance models that can be applied to automate microbiology diagnostics, impacting public health and clinical practice.
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    Item type:Publication,
    A Deep Learning Model for Bacterial Classification Using Big Transfer (BiT)
    (2024-01-01)
    Visitsattaponge, Sarinporn
    ;
    Bunkum, Manao
    ;
    Pintavirooj, Chuchart
    ;
    Paing, May Phu
    Identification and classification of bacterial genera and species are very important for medical prevention, diagnosis, and treatment. However, due to microbial diversity and high variability in appearance, the manual classification of bacteria is a challenging and time-consuming task. This paper aims to facilitate such a troublesome task using deep learning techniques. Through the utilization of a deep learning model, specifically a Big Transfer (BiT) combined with graph Laplacian-based data cleaning and weight initialization based-rectified linear unit (WIB-Relu) activation, we have developed an accurate bacteria classification model. We have tested our proposed method on a public dataset of microscopic bacteria images, called the Digital Images of Bacteria Species (DIBaS), and achieved promising results with an accuracy of 99.11%, precision of 99.31%, recall of 99.09%, and F1 score of 99.06%, respectively. Moreover, the proposed bacteria classification performed well regardless of the size of the training data. We investigated its generalizability not only on the original dataset but also on the few shots (5-shots, 2-shots, and 1-shot) and augmented datasets.