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Optimization of astaxanthin production by Rhodotorula toruloides CB6-10/1 using response surface methodology and its genome analysis
(2026-02-01)
Butsararattanagomen, Pornthipa
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Tanasupawat, Somboon
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Kingkaew, Engkarat
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Kotatha, Ditpon
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Soontorngun, Nitnipa
Astaxanthin is a valuable carotenoid with potent antioxidant properties and has broad applications in the pharmaceutical, nutraceutical, and cosmetic industries. In this study, Rhodotorula toruloides CB6-10/1, isolated from Canna indica L. flowers, was evaluated for astaxanthin production. The orange-red pigment was confirmed as astaxanthin via thin-layer chromatography and high-performance liquid chromatography, with quantification performed by spectrophotometry. Comprehensive genome analysis and production optimization of R. toruloides CB6-10/1 confirmed the presence of key astaxanthin biosynthesis genes, such as CrtYB, CrtI, CrtW, CrtZ, and CrtR, which facilitate the conversion of β-carotene to astaxanthin through hydroxylation and ketolation. The key parameters, including carbon and nitrogen sources, their concentrations, trace elements, agitation speed, and pH, were systematically evaluated to optimize production. Although copper appeared beneficial in the Plackett–Burman screening, its effect and those of other metals were not significant. Optimization using Response Surface Methodology for cost-effective nitrogen sources determined that a combination of 1.10 g/L yeast extract, 10.0 g/L peptone, and 0.50 g/L ammonium sulfate yielded the maximum astaxanthin production of 4.728 mg/L, under cultivation conditions of pH 4.5, 200 rpm, and 30 g/L glucose, representing a fourfold increase compared with the basal medium. This optimization not only enhances pigment production efficiency but also reduces dependency on costly trace elements, improving process scalability and economic feasibility. Overall, these results demonstrate R. toruloides CB6-10/1 as a promising microbial source for sustainable astaxanthin production with potential further applications.
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Optimization of betalain extraction from dragon fruit (Hylocereus undatus) peel and effect of pH on its properties
(2025-02-01)
Permana, Lasuardi
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Sriprom, Pongsert
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Manamoongmongkol, Kanjana
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Phumjan, Lamphung
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Assawasaengrat, Pornsawan
Dragon fruit peels are wastes produced in the food industry and contain abundant phytochemicals. In this study, betalain extraction from dragon fruit peels was optimized using water as a safe and environment-friendly solvent. The Box-Behnken design was used to investigate the effects of different pH (2.0–6.0), temperatures (30–60 °C), times (10–60 min), and solid-to-liquid ratios (1:15–1:40 by weight) on betalain extraction from dragon fruit peels. The betalain extracted under the optimized conditions was then studied for its properties across a wide pH range of 2.0 to 13.0. The results showed that the solid-to-liquid ratio had the most significant effect on betacyanin extraction as the major component of betalain in dragon fruit peels. The optimal extraction conditions were determined to be a pH of 3.6, temperature of 30 °C, extraction time of 10 min, and a solid-to-liquid ratio of 1:15. The expected betacyanin content under these conditions is 72.37 mg/L. A significant change in the CIELAB parameter of the extracted betalain was observed, with marked differences in the L*, a*, and b* values as the pH increased. This color change may be attributed to the degradation of betacyanin into betalamic acid. This study demonstrated the potential of a safe and eco-friendly process for extracting the natural pigment betacyanin from this agricultural waste material and the potential use of betalain in pH-dependent products.
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Antidiabetic Property Optimization from Green Leafy Vegetables Using Ultrasound-Assisted Extraction to Improve Cracker Production
(2024-01-01)
Maser, Wahyu Haryati
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Maiyah, Nur
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Karnjanapratum, Supatra
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Nukthamna, Pikunthong
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Thompson, Anthony Keith
Here we test a method of incorporating of plant extracts into popular snack foods to help control diabetes. Since some fresh vegetables contain antidiabetic compounds, ultrasound-assisted extraction was used to optimize their extraction of from spring onions, bunching onions, and celery for later incorporation into crackers. We compared various concentrations of ethanol used during extraction, after which they were exposed to an ultrasound processor whose amplitude and sonication time were also varied. The optimal extraction conditions were found to be an ethanol concentration of 44.08%, an amplitude of 80%, and a sonication time of 30 min. This resulted in the highest level of α-glucosidase inhibitory activity (i.e., 1,449.73 mmol ACE/g) and the highest extraction yield (i.e., 24.16%). The extract produced from these optimum conditions was then used as a constituent component of crackers at 0.625%, 1.25%, or 2.5% w/w. These biscuits were then produced at baking temperatures of 140°C, 150°C, or 160°C. We then measured the physical characteristics and bioactivities of sample biscuits from each treatment. We found that biscuits containing 2.5% vegetable combination extract and baked at 140°C had the highest total phenolic content, the strongest antioxidant performance, and showed the most substantial antidiabetic and antiobesity effects. Here we establish conditions for the effective extraction of antidiabetic functional ingredients via ultrasound from green leafy vegetables. We also provide a method of using these ingredients to prepare crackers with the aim of developing a functional antidiabetic snack food.
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EFFECT OF FOAMING AGENTS AND DRYING TEMPERATURE ON DRYING RATE AND QUALITY OF FOAM-MAT DRIED PAPAYA POWDER
(2024-01-01)
Van Hao, Hong
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Thuy, Nguyen Minh
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Giau, Tran Ngoc
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Van Tai, Ngo
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Minh, Vo Quang
Recently, foam-mat drying is being considered a mild drying technique, requiring low investment costs but being particularly highly effective. This drying method is very useful for liquid or semi-liquid food products that are highly viscous and easily change qualities by heat. Liquid or finely ground foods are foamed with the assistance of surfactants (foam-creating and foam-stabilizing agents are egg albumin and carboxymethyl cellulose, respectively), followed by drying of the foam with hot air. Box-Behnken design (BBD) of response surface methodology (RSM) was designed in this study, with 3 factors and 3 levels, including egg albumin content (6–10%), carboxymethyl cellulose (CMC) (0.1–0.5%) and drying temperature (50–70oC). The drying rate and β-carotene content of foam and product, respectively, are the indicators observed in 18 experimental runs and 6 repetitions at the central point. Use target settings for each response based on high β-carotene content and highest drying rate to produce a high-quality papaya powder product with the shortest drying time. The optimal and feasible experimental conditions for drying foam mats were achieved with albumin concentration, CMC and optimal drying temperature at 9.07%, 0.39% and 62oC, respectively. From these optimal conditions, the optimal values of drying rate and β-carotene content were achieved as 3.41 g water/g dry matter/min and 37.95 µg/g, respectively. This study has confirmed that foam drying is a feasible method to produce good quality papaya powder, the product maintains the natural color of the original material, low moisture (8.5%) and water activity (0.35), and high rehydration ratio (4.05) when drying at 62oC for 3 hours.

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