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Multi-objective optimization of lignocellulolytic enzyme cocktail production from Pseudolagarobasidium acaciicola TDW-48 by artificial neural network-genetic algorithm (ANN-GA) strategy and its application in lignocellulose waste bioconversion
(2025-03-01)
Luong, Thi Thu Huong
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Poeaim, Supattra
A massive amount of lignocellulose waste is generated annually, causing many environmental concerns. The bioconversion of these wastes into value-added products by the lignocellulolytic enzymes (LCE) is one of the effective and environmental approaches. However, the use of LCE has not been extended due to high costs. This study aimed to enhance the yield of crude LCE cocktail production from Pseudolagarobasidium acaciicola TDW-48 by optimizing cultural conditions using statistical tools. Firstly, the effect of cultural factors on LCE production was identified through the Plackett-Buman design. Then, the artificial neural network-genetic algorithm (ANN-GA) strategy was applied to optimize the significant factors. The result shows that the production of carboxymethyl cellulase (CMCase), xylanase, and laccase responded differently to cultural conditions. Among these, five factors (incubation time, water content, medium pH, glucose, and CuSO<inf>4</inf> concentration) were identified to have significant effects on enzyme activities. The ANN-GA optimization with a neuron network architecture (5-23-3) successfully modeled the crude LCE cocktail production, where the R-value achieved 0.98369 for the total dataset. A set of optimum conditions was proposed with an incubation time of 8 days, 72.6% water content, medium pH at 2.97, 0.5% glucose, and 0.53 g/L of CuSO<inf>4</inf>. With the above conditions, P. acaciicola TDW-48 could produce 23.97 U/g of CMCase, 26.02 U/g of xylanase, and 139.11 U/g of laccase, which enhanced 14.4%, 8.7%, and 405% activity, respectively, compared with non-optimization. In addition, the P. acaciicola TDW-48’s crude LCE cocktail performed a high bioconversion efficiency on lignocellulose waste, the reducing sugar yield achieved 327.29 mg/g on rice straw, 308.02 mg/g on rice husk, and 312.29 mg/g on corn stover after 8-h incubation. These results provided a highly effective approach for LCE production with multi-objective optimization based on an artificial intelligence platform and supported the reuse of lignocellulose waste toward the eco-friendly strategy.
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Application of baby corn husk as a biological sustainable feedstock for the production of cellulase and xylanase by Lentinus squarrosulus Mont.
(2023-02-01)
Vichitraka, Asanee
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Somboon, Pichayada
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Tantratian, Sumate
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Onmankhong, Jiraporn
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Sirisomboon, Panmanas
In an effort to use baby corn husk (BCH) as a sustainable feedstock for cellulase and xylanase production by the Lentinus squarrosulus Mont. isolate LS-YA (LSM-LS-YA), a suitable pretreatment method and fermentation strategies were developed. BCH pretreated with 1 M sodium hydroxide for 90 min, an alkaline pretreatment, exemplified an appropriate pretreatment method. In a 10-L external Venturi injector bioreactor, the highest cellulase and xylanase production was 4.12 ± 0.36 unit/mL and 6.15 ± 0.36 unit/mL, respectively, when 1 g/L diammonium hydrogen phosphate was used as the nitrogen source and the aeration rate was controlled at 0.2 vvm. This study provides an informative perspective on the production of cellulase and xylanase from agricultural lignocellulosic materials, which could reduce agricultural waste while supporting a zero-waste circular economy, and this fermentation process would be applicable to larger-scale production.
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Cellulase pretreated palm decanter cake for feeding of black soldier fly larvae in triggering bioaccumulation of protein and lipid into biodiesel productions
(2022-10-01)
Jing Lim, Jia
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Seng Liew, Chin
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Raksasat, Ratchaprapa
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Merican, Zulkifli M.A.
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Kiatkittipong, Kunlanan
For every ton of palm oil produced, approximately 0.2 tons of palm decanter cake (PDC) waste is generated. Hence, there is a huge opportunity to valorize this organic waste, i.e., via the deployment of black soldier fly larvae (BSFL), which has been widely employed to convert various organic wastes into larval proteins and lipids. However, the PDC is mainly made up of lignocellulosic materials that are hard to digest by the BSFL. Therefore, this work attempted to grow BSFL in cellulase-pretreated PDC; thereby, providing an alternative solution to manage PDC waste. Results had shown that the cellulase pretreatment was effective in breaking down cellulose into glucose molecules, especially with higher cellulase dosage and longer treatment duration of up until 48 h. Subsequently, the maximum BSFL growth was found at 6.56 ± 2.69 mg/larva when being fed with PDC pre-treated by 1.0 wt% of cellulase for 72 h; which was about 4 mg higher than the controlled larva. Using a similar substrate, the highest protein yield and lipid yield from BSFL were attained at 1.63 ± 0.11 mg/larva (22.4 wt%) and 5.12 ± 1.01 mg/larva (69.9 wt%), respectively. In terms of biodiesel quality, a huge presence of saturated fatty acids had made the BSFL-based biodiesel oxidatively stable.
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Cellulase production by Aspergillus Niger ATCC 16888 on copra waste from coconut milk process in layered packed-bed bioreactor
(2018-07-01)
Chysirichote, T.
Solid-state fermentation (SSF) has been used to produce value-added products from agricultural and food processing wastes, but the majority of SSF production has been conducted on a small scale due to the limitations of heat and mass transfer. This research aimed to reduce the temperature changes in packed-bed bioreactor during the cellulase fermentation of Aspergillus Niger ATCC 16888 on copra waste from coconut milk process. The three configurations of bioreactor used in this study consisted of one-(control), two- and three-layered packed-bed bioreactors. Total volumes of packed-bed bioreactor and bed material were 127 and 57 L, respectively. The sterilized air 30 °C was forced from the bottom of bioreactor at 0.20 vvm. The highest cellulase production (5.0 ± 0.4 FPU g<sup>-1</sup> <inf>DS</inf>) was obtained from the bottom zone of the three-layered bioreactor after fermenting for 1 d. However, the greatest growth was found at the top one, in which a large amount of aerial mycelium was detected.
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Enzymatic degradation of modified gelatin and carboxymethyl cellulose scaffolds
(2018-01-01)
Sutjaritvorakul, Thanawat
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Wiwatwongwana, Fasai
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Imsuwan, Pattareewan
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Whalley, Anthony J.S.
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Chutipaijit, Sutee
The modification of biological and synthetic biopolymer provides suitable properties for bone and tissue engineering. Modified gelatin and carboxymethyl cellulose (CMC) scaffolds are interesting biomaterials for applying as artificial dermal skin. The objective of this research was to investigate the enzymatic degradation of gelatin and carboxymethyl cellulose scaffolds. The samples were prepared in different compositions of gelatin and CMC (Gelatin:CMC) (w/w) such as 9:1, 8:2, 7:3 and 6:4. The enzymatic degradation test was undertaken using cellulase produced by Aspergillus niger. The results demonstrated that GC64 (Gelatin:CMC, 6:4) exhibited the highest degree of enzymatic degradation and the behavior of the degradation was predicated based on water absorption ability. Moreover, the morphology of degraded residues were investigated by scanning electron microscopy (SEM).
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Isolation of a gene encoding a cellulolytic enzyme from swamp buffalo rumen metagenomes and its cloning and expression in escherichia coli
(2012-01-01)
Cheema, Tanzeem Akbar
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Jirajaroenrat, Kanya
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Sirinarumitr, Theerapol
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Rakshit, Sudip K.
Ruminants are capable of hydrolyzing lignocellulosic residues to absorbable sugars by virtue of the microbial communities residing in their rumen. However, large sections of such microbial communities are not yet culturable using conventional laboratory techniques. Therefore in the present study, the metagenomic DNA of swamp buffalo (Bubalus bubalis) rumen contents was explored using culture-independent techniques. The consensus regions of glycosyl hydrolase 5 (GH5) family of cellulases were used as primers for PCR amplification. A full-length metagenomic cellulase gene, Umcel5B29, with a complete open reading frame (ORF) of 1611bp was identified. The similarity search analysis revealed that Umcel5B29 is closely related to the cellulases (73% to 98% similarity) of ruminal unculturable microorganisms, indicating its phylogenetic origin. Further analysis indicated that Umcel5B29 does not contain a carbohydrate binding module (CBM). Subsequently, Umcel5B29 was overexpressed in Escherichia coli. The recombinant enzyme worked optimally at pH 5.5 and 45C, a condition similar to the buffalo's rumen. However, the enzyme retained more than 70% of its maximal activity after incubation at <inf>p</inf>H 4-7 and more than 50% maximal activity after incubation at 30-60°C for 30min. These characteristics render Umcel5B29 as a potential candidate for the bio-stoning process of denim. © 2012 Taylor and Francis Group, LLC.

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